Ilheus virus isolation in the Pantanal, west-central Brazil.
The wetlands of the Brazilian Pantanal host large concentrations of diverse wildlife species and hematophagous arthropods, conditions that favor the circulation of zoonotic arboviruses. A recent study from the Nhecolândia sub-region of Pantanal reported serological evidence of various flaviviruses,...
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ftdoajarticles:oai:doaj.org/article:92864b12ad6b49af8a3e22e2c68111b6 2023-05-15T15:06:34+02:00 Ilheus virus isolation in the Pantanal, west-central Brazil. Alex Pauvolid-Corrêa Joan L Kenney Dinair Couto-Lima Zilca M S Campos Hermann G Schatzmayr Rita M R Nogueira Aaron C Brault Nicholas Komar 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002318 https://doaj.org/article/92864b12ad6b49af8a3e22e2c68111b6 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3715421?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002318 https://doaj.org/article/92864b12ad6b49af8a3e22e2c68111b6 PLoS Neglected Tropical Diseases, Vol 7, Iss 7, p e2318 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002318 2022-12-30T21:04:27Z The wetlands of the Brazilian Pantanal host large concentrations of diverse wildlife species and hematophagous arthropods, conditions that favor the circulation of zoonotic arboviruses. A recent study from the Nhecolândia sub-region of Pantanal reported serological evidence of various flaviviruses, including West Nile virus and Ilheus virus (ILHV). According to the age of seropositive horses, at least three flaviviruses, including ILHV, circulated in the Brazilian Pantanal between 2005 and 2009. To extend this study, we collected 3,234 adult mosquitoes of 16 species during 2009 and 2010 in the same sub-region. Mosquito pool homogenates were assayed for infectious virus on C6/36 and Vero cell monolayers and also tested for flaviviral RNA by a group-specific real-time RT-PCR. One pool containing 50 non-engorged female specimens of Aedes scapularis tested positive for ILHV by culture and for ILHV RNA by real-time RT-PCR, indicating a minimum infection rate of 2.5 per 1000. Full-length genomic sequence exhibited 95% identity to the only full genome sequence available for ILHV. The present data confirm the circulation of ILHV in the Brazilian Pantanal. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 7 7 e2318 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Alex Pauvolid-Corrêa Joan L Kenney Dinair Couto-Lima Zilca M S Campos Hermann G Schatzmayr Rita M R Nogueira Aaron C Brault Nicholas Komar Ilheus virus isolation in the Pantanal, west-central Brazil. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The wetlands of the Brazilian Pantanal host large concentrations of diverse wildlife species and hematophagous arthropods, conditions that favor the circulation of zoonotic arboviruses. A recent study from the Nhecolândia sub-region of Pantanal reported serological evidence of various flaviviruses, including West Nile virus and Ilheus virus (ILHV). According to the age of seropositive horses, at least three flaviviruses, including ILHV, circulated in the Brazilian Pantanal between 2005 and 2009. To extend this study, we collected 3,234 adult mosquitoes of 16 species during 2009 and 2010 in the same sub-region. Mosquito pool homogenates were assayed for infectious virus on C6/36 and Vero cell monolayers and also tested for flaviviral RNA by a group-specific real-time RT-PCR. One pool containing 50 non-engorged female specimens of Aedes scapularis tested positive for ILHV by culture and for ILHV RNA by real-time RT-PCR, indicating a minimum infection rate of 2.5 per 1000. Full-length genomic sequence exhibited 95% identity to the only full genome sequence available for ILHV. The present data confirm the circulation of ILHV in the Brazilian Pantanal. |
format |
Article in Journal/Newspaper |
author |
Alex Pauvolid-Corrêa Joan L Kenney Dinair Couto-Lima Zilca M S Campos Hermann G Schatzmayr Rita M R Nogueira Aaron C Brault Nicholas Komar |
author_facet |
Alex Pauvolid-Corrêa Joan L Kenney Dinair Couto-Lima Zilca M S Campos Hermann G Schatzmayr Rita M R Nogueira Aaron C Brault Nicholas Komar |
author_sort |
Alex Pauvolid-Corrêa |
title |
Ilheus virus isolation in the Pantanal, west-central Brazil. |
title_short |
Ilheus virus isolation in the Pantanal, west-central Brazil. |
title_full |
Ilheus virus isolation in the Pantanal, west-central Brazil. |
title_fullStr |
Ilheus virus isolation in the Pantanal, west-central Brazil. |
title_full_unstemmed |
Ilheus virus isolation in the Pantanal, west-central Brazil. |
title_sort |
ilheus virus isolation in the pantanal, west-central brazil. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0002318 https://doaj.org/article/92864b12ad6b49af8a3e22e2c68111b6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 7, p e2318 (2013) |
op_relation |
http://europepmc.org/articles/PMC3715421?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002318 https://doaj.org/article/92864b12ad6b49af8a3e22e2c68111b6 |
op_doi |
https://doi.org/10.1371/journal.pntd.0002318 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
7 |
container_issue |
7 |
container_start_page |
e2318 |
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1766338147201843200 |