Anti-Virulence Activity of the Cell-Free Supernatant of the Antarctic Bacterium Psychrobacter sp. TAE2020 against Pseudomonas aeruginosa Clinical Isolates from Cystic Fibrosis Patients
Pseudomonas aeruginosa is an opportunistic pathogen often involved in airway infections of cystic fibrosis (CF) patients. Its pathogenicity is related to several virulence factors, such as biofilm formation, motility and production of toxins and proteases. The expression of these virulence factors i...
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ftdoajarticles:oai:doaj.org/article:9256e34a9fe54365b89c2115bd696b99 2023-05-15T13:48:10+02:00 Anti-Virulence Activity of the Cell-Free Supernatant of the Antarctic Bacterium Psychrobacter sp. TAE2020 against Pseudomonas aeruginosa Clinical Isolates from Cystic Fibrosis Patients Rosanna Papa Gianluca Vrenna Caterina D’Angelo Angela Casillo Michela Relucenti Orlando Donfrancesco Maria Michela Corsaro Ersilia Vita Fiscarelli Vanessa Tuccio Guarna Assanti Maria Luisa Tutino Ermenegilda Parrilli Marco Artini Laura Selan 2021-08-01T00:00:00Z https://doi.org/10.3390/antibiotics10080944 https://doaj.org/article/9256e34a9fe54365b89c2115bd696b99 EN eng MDPI AG https://www.mdpi.com/2079-6382/10/8/944 https://doaj.org/toc/2079-6382 doi:10.3390/antibiotics10080944 2079-6382 https://doaj.org/article/9256e34a9fe54365b89c2115bd696b99 Antibiotics, Vol 10, Iss 944, p 944 (2021) Pseudomonas aeruginosa cystic fibrosis anti-virulence SEM motility biofilm Therapeutics. Pharmacology RM1-950 article 2021 ftdoajarticles https://doi.org/10.3390/antibiotics10080944 2022-12-31T06:03:28Z Pseudomonas aeruginosa is an opportunistic pathogen often involved in airway infections of cystic fibrosis (CF) patients. Its pathogenicity is related to several virulence factors, such as biofilm formation, motility and production of toxins and proteases. The expression of these virulence factors is controlled by quorum sensing (QS). Thus, QS inhibition is considered a novel strategy for the development of antipathogenic compounds acting on specific bacterial virulence programs without affecting bacterial vitality. In this context, cold-adapted marine bacteria living in polar regions represent an untapped reservoir of biodiversity endowed with an interesting chemical repertoire. In this paper, we investigated the biological activity of a supernatant derived from a novel Antarctic bacterium (SN_TAE2020) against specific virulence factors produced by P. aeruginosa strains isolated from FC patients. Our results clearly show a reduction in pyocyanin and protease production in the presence of SN_TAE2020. Finally, SN_TAE2020 was also able to strongly affect swarming and swimming motility for almost all tested strains. Furthermore, the effect of SN_TAE2020 was investigated on biofilm growth and texture, captured by SEM analysis. In consideration of the novel results obtained on clinical strains, polar bacteria might represent potential candidates for the discovery of new compounds limiting P. aeruginosa virulence in CF patients. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Antibiotics 10 8 944 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Pseudomonas aeruginosa cystic fibrosis anti-virulence SEM motility biofilm Therapeutics. Pharmacology RM1-950 |
spellingShingle |
Pseudomonas aeruginosa cystic fibrosis anti-virulence SEM motility biofilm Therapeutics. Pharmacology RM1-950 Rosanna Papa Gianluca Vrenna Caterina D’Angelo Angela Casillo Michela Relucenti Orlando Donfrancesco Maria Michela Corsaro Ersilia Vita Fiscarelli Vanessa Tuccio Guarna Assanti Maria Luisa Tutino Ermenegilda Parrilli Marco Artini Laura Selan Anti-Virulence Activity of the Cell-Free Supernatant of the Antarctic Bacterium Psychrobacter sp. TAE2020 against Pseudomonas aeruginosa Clinical Isolates from Cystic Fibrosis Patients |
topic_facet |
Pseudomonas aeruginosa cystic fibrosis anti-virulence SEM motility biofilm Therapeutics. Pharmacology RM1-950 |
description |
Pseudomonas aeruginosa is an opportunistic pathogen often involved in airway infections of cystic fibrosis (CF) patients. Its pathogenicity is related to several virulence factors, such as biofilm formation, motility and production of toxins and proteases. The expression of these virulence factors is controlled by quorum sensing (QS). Thus, QS inhibition is considered a novel strategy for the development of antipathogenic compounds acting on specific bacterial virulence programs without affecting bacterial vitality. In this context, cold-adapted marine bacteria living in polar regions represent an untapped reservoir of biodiversity endowed with an interesting chemical repertoire. In this paper, we investigated the biological activity of a supernatant derived from a novel Antarctic bacterium (SN_TAE2020) against specific virulence factors produced by P. aeruginosa strains isolated from FC patients. Our results clearly show a reduction in pyocyanin and protease production in the presence of SN_TAE2020. Finally, SN_TAE2020 was also able to strongly affect swarming and swimming motility for almost all tested strains. Furthermore, the effect of SN_TAE2020 was investigated on biofilm growth and texture, captured by SEM analysis. In consideration of the novel results obtained on clinical strains, polar bacteria might represent potential candidates for the discovery of new compounds limiting P. aeruginosa virulence in CF patients. |
format |
Article in Journal/Newspaper |
author |
Rosanna Papa Gianluca Vrenna Caterina D’Angelo Angela Casillo Michela Relucenti Orlando Donfrancesco Maria Michela Corsaro Ersilia Vita Fiscarelli Vanessa Tuccio Guarna Assanti Maria Luisa Tutino Ermenegilda Parrilli Marco Artini Laura Selan |
author_facet |
Rosanna Papa Gianluca Vrenna Caterina D’Angelo Angela Casillo Michela Relucenti Orlando Donfrancesco Maria Michela Corsaro Ersilia Vita Fiscarelli Vanessa Tuccio Guarna Assanti Maria Luisa Tutino Ermenegilda Parrilli Marco Artini Laura Selan |
author_sort |
Rosanna Papa |
title |
Anti-Virulence Activity of the Cell-Free Supernatant of the Antarctic Bacterium Psychrobacter sp. TAE2020 against Pseudomonas aeruginosa Clinical Isolates from Cystic Fibrosis Patients |
title_short |
Anti-Virulence Activity of the Cell-Free Supernatant of the Antarctic Bacterium Psychrobacter sp. TAE2020 against Pseudomonas aeruginosa Clinical Isolates from Cystic Fibrosis Patients |
title_full |
Anti-Virulence Activity of the Cell-Free Supernatant of the Antarctic Bacterium Psychrobacter sp. TAE2020 against Pseudomonas aeruginosa Clinical Isolates from Cystic Fibrosis Patients |
title_fullStr |
Anti-Virulence Activity of the Cell-Free Supernatant of the Antarctic Bacterium Psychrobacter sp. TAE2020 against Pseudomonas aeruginosa Clinical Isolates from Cystic Fibrosis Patients |
title_full_unstemmed |
Anti-Virulence Activity of the Cell-Free Supernatant of the Antarctic Bacterium Psychrobacter sp. TAE2020 against Pseudomonas aeruginosa Clinical Isolates from Cystic Fibrosis Patients |
title_sort |
anti-virulence activity of the cell-free supernatant of the antarctic bacterium psychrobacter sp. tae2020 against pseudomonas aeruginosa clinical isolates from cystic fibrosis patients |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/antibiotics10080944 https://doaj.org/article/9256e34a9fe54365b89c2115bd696b99 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Antibiotics, Vol 10, Iss 944, p 944 (2021) |
op_relation |
https://www.mdpi.com/2079-6382/10/8/944 https://doaj.org/toc/2079-6382 doi:10.3390/antibiotics10080944 2079-6382 https://doaj.org/article/9256e34a9fe54365b89c2115bd696b99 |
op_doi |
https://doi.org/10.3390/antibiotics10080944 |
container_title |
Antibiotics |
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10 |
container_issue |
8 |
container_start_page |
944 |
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1766248814295908352 |