Mosquito-bite infection of humanized mice with chikungunya virus produces systemic disease with long-term effects.
Chikungunya virus (CHIKV) is an emerging, mosquito-borne alphavirus responsible for acute to chronic arthralgias and neuropathies. Although it originated in central Africa, recent reports of disease have come from many parts of the world, including the Americas. While limiting human CHIKV cases thro...
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ftdoajarticles:oai:doaj.org/article:924cd28ae4dc4e22b1ca58d4d1d30cf2 2023-05-15T15:09:21+02:00 Mosquito-bite infection of humanized mice with chikungunya virus produces systemic disease with long-term effects. Brianne M Hibl Natalie J M Dailey Garnes Alexander R Kneubehl Megan B Vogt Jennifer L Spencer Clinton Rebecca R Rico-Hesse 2021-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009427 https://doaj.org/article/924cd28ae4dc4e22b1ca58d4d1d30cf2 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009427 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009427 https://doaj.org/article/924cd28ae4dc4e22b1ca58d4d1d30cf2 PLoS Neglected Tropical Diseases, Vol 15, Iss 6, p e0009427 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009427 2022-12-31T02:14:19Z Chikungunya virus (CHIKV) is an emerging, mosquito-borne alphavirus responsible for acute to chronic arthralgias and neuropathies. Although it originated in central Africa, recent reports of disease have come from many parts of the world, including the Americas. While limiting human CHIKV cases through mosquito control has been used, it has not been entirely successful. There are currently no licensed vaccines or treatments specific for CHIKV disease, thus more work is needed to develop effective countermeasures. Current animal research on CHIKV is often not representative of human disease. Most models use CHIKV needle inoculation via unnatural routes to create immediate viremia and localized clinical signs; these methods neglect the natural route of transmission (the mosquito vector bite) and the associated human immune response. Since mosquito saliva has been shown to have a profound effect on viral pathogenesis, we evaluated a novel model of infection that included the natural vector, Aedes species mosquitoes, transmitting CHIKV to mice containing components of the human immune system. Humanized mice infected by 3-6 mosquito bites showed signs of systemic infection, with demonstrable viremia (by qRT-PCR and immunofluorescent antibody assay), mild to moderate clinical signs (by observation, histology, and immunohistochemistry), and immune responses consistent with human infection (by flow cytometry and IgM ELISA). This model should give a better understanding of human CHIKV disease and allow for more realistic evaluations of mechanisms of pathogenesis, prophylaxis, and treatments. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 6 e0009427 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Brianne M Hibl Natalie J M Dailey Garnes Alexander R Kneubehl Megan B Vogt Jennifer L Spencer Clinton Rebecca R Rico-Hesse Mosquito-bite infection of humanized mice with chikungunya virus produces systemic disease with long-term effects. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Chikungunya virus (CHIKV) is an emerging, mosquito-borne alphavirus responsible for acute to chronic arthralgias and neuropathies. Although it originated in central Africa, recent reports of disease have come from many parts of the world, including the Americas. While limiting human CHIKV cases through mosquito control has been used, it has not been entirely successful. There are currently no licensed vaccines or treatments specific for CHIKV disease, thus more work is needed to develop effective countermeasures. Current animal research on CHIKV is often not representative of human disease. Most models use CHIKV needle inoculation via unnatural routes to create immediate viremia and localized clinical signs; these methods neglect the natural route of transmission (the mosquito vector bite) and the associated human immune response. Since mosquito saliva has been shown to have a profound effect on viral pathogenesis, we evaluated a novel model of infection that included the natural vector, Aedes species mosquitoes, transmitting CHIKV to mice containing components of the human immune system. Humanized mice infected by 3-6 mosquito bites showed signs of systemic infection, with demonstrable viremia (by qRT-PCR and immunofluorescent antibody assay), mild to moderate clinical signs (by observation, histology, and immunohistochemistry), and immune responses consistent with human infection (by flow cytometry and IgM ELISA). This model should give a better understanding of human CHIKV disease and allow for more realistic evaluations of mechanisms of pathogenesis, prophylaxis, and treatments. |
format |
Article in Journal/Newspaper |
author |
Brianne M Hibl Natalie J M Dailey Garnes Alexander R Kneubehl Megan B Vogt Jennifer L Spencer Clinton Rebecca R Rico-Hesse |
author_facet |
Brianne M Hibl Natalie J M Dailey Garnes Alexander R Kneubehl Megan B Vogt Jennifer L Spencer Clinton Rebecca R Rico-Hesse |
author_sort |
Brianne M Hibl |
title |
Mosquito-bite infection of humanized mice with chikungunya virus produces systemic disease with long-term effects. |
title_short |
Mosquito-bite infection of humanized mice with chikungunya virus produces systemic disease with long-term effects. |
title_full |
Mosquito-bite infection of humanized mice with chikungunya virus produces systemic disease with long-term effects. |
title_fullStr |
Mosquito-bite infection of humanized mice with chikungunya virus produces systemic disease with long-term effects. |
title_full_unstemmed |
Mosquito-bite infection of humanized mice with chikungunya virus produces systemic disease with long-term effects. |
title_sort |
mosquito-bite infection of humanized mice with chikungunya virus produces systemic disease with long-term effects. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009427 https://doaj.org/article/924cd28ae4dc4e22b1ca58d4d1d30cf2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 6, p e0009427 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009427 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009427 https://doaj.org/article/924cd28ae4dc4e22b1ca58d4d1d30cf2 |
op_doi |
https://doi.org/10.1371/journal.pntd.0009427 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
15 |
container_issue |
6 |
container_start_page |
e0009427 |
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1766340566451224576 |