Taenia solium Infections in a rural area of Eastern Zambia-a community based study.
BACKGROUND: Taenia solium taeniosis/cysticercosis is a parasitic infection occurring in many developing countries. Data on the status of human infections in Zambia is largely lacking. We conducted a community-based study in Eastern Zambia to determine the prevalence of human taeniosis and cysticerco...
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ftdoajarticles:oai:doaj.org/article:922f74ebe81042fe9f63f953b541cc10 2023-05-15T15:16:07+02:00 Taenia solium Infections in a rural area of Eastern Zambia-a community based study. Kabemba E Mwape Isaac K Phiri Nicolas Praet John B Muma Gideon Zulu Peter Van den Bossche Reginald de Deken Niko Speybroeck Pierre Dorny Sarah Gabriël 2012-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001594 https://doaj.org/article/922f74ebe81042fe9f63f953b541cc10 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3313923?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001594 https://doaj.org/article/922f74ebe81042fe9f63f953b541cc10 PLoS Neglected Tropical Diseases, Vol 6, Iss 3, p e1594 (2012) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2012 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001594 2022-12-30T22:26:16Z BACKGROUND: Taenia solium taeniosis/cysticercosis is a parasitic infection occurring in many developing countries. Data on the status of human infections in Zambia is largely lacking. We conducted a community-based study in Eastern Zambia to determine the prevalence of human taeniosis and cysticercosis in a rural community. METHODS AND FINDINGS: Stool and serum samples were collected from willing participants. Geographical references of the participants' households were determined and household questionnaires administered. Taeniosis was diagnosed in stool samples by coprology and by the polyclonal antibody-based copro-antigen enzyme-linked immunosorbent assay (copro-Ag ELISA), while cysticercosis was diagnosed in serum by the B158/B60 monoclonal antibody-based antigen ELISA (sero-Ag ELISA). Identification of the collected tapeworm after niclosamide treatment and purgation was done using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A total of 255 households from 20 villages participated in the study, 718 stool and 708 serum samples were collected and examined. Forty-five faecal samples (6.3%) were found positive for taeniosis on copro-Ag ELISA while circulating cysticercus antigen was detected in 5.8% (41/708) individuals. The tapeworm recovered from one of the cases was confirmed to be T. solium on PCR-RFLP. Seropositivity (cysticercosis) was significantly positively related to age (p = 0.00) and to copro-Ag positivity (taeniosis) (p = 0.03) but not to gender. Change point analysis revealed that the frequency of cysticercus antigens increased significantly in individuals above the age of 30. Copro-Ag positivity was not related to age or gender. The following risk factors were noted to be present in the study community: free-range pig husbandry system and poor sanitation with 47.8% of the households visited lacking latrines. CONCLUSIONS: This study has recorded high taeniosis and cysticercosis prevalences and identified the need for further studies on transmission dynamics and ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 6 3 e1594 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Kabemba E Mwape Isaac K Phiri Nicolas Praet John B Muma Gideon Zulu Peter Van den Bossche Reginald de Deken Niko Speybroeck Pierre Dorny Sarah Gabriël Taenia solium Infections in a rural area of Eastern Zambia-a community based study. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: Taenia solium taeniosis/cysticercosis is a parasitic infection occurring in many developing countries. Data on the status of human infections in Zambia is largely lacking. We conducted a community-based study in Eastern Zambia to determine the prevalence of human taeniosis and cysticercosis in a rural community. METHODS AND FINDINGS: Stool and serum samples were collected from willing participants. Geographical references of the participants' households were determined and household questionnaires administered. Taeniosis was diagnosed in stool samples by coprology and by the polyclonal antibody-based copro-antigen enzyme-linked immunosorbent assay (copro-Ag ELISA), while cysticercosis was diagnosed in serum by the B158/B60 monoclonal antibody-based antigen ELISA (sero-Ag ELISA). Identification of the collected tapeworm after niclosamide treatment and purgation was done using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A total of 255 households from 20 villages participated in the study, 718 stool and 708 serum samples were collected and examined. Forty-five faecal samples (6.3%) were found positive for taeniosis on copro-Ag ELISA while circulating cysticercus antigen was detected in 5.8% (41/708) individuals. The tapeworm recovered from one of the cases was confirmed to be T. solium on PCR-RFLP. Seropositivity (cysticercosis) was significantly positively related to age (p = 0.00) and to copro-Ag positivity (taeniosis) (p = 0.03) but not to gender. Change point analysis revealed that the frequency of cysticercus antigens increased significantly in individuals above the age of 30. Copro-Ag positivity was not related to age or gender. The following risk factors were noted to be present in the study community: free-range pig husbandry system and poor sanitation with 47.8% of the households visited lacking latrines. CONCLUSIONS: This study has recorded high taeniosis and cysticercosis prevalences and identified the need for further studies on transmission dynamics and ... |
format |
Article in Journal/Newspaper |
author |
Kabemba E Mwape Isaac K Phiri Nicolas Praet John B Muma Gideon Zulu Peter Van den Bossche Reginald de Deken Niko Speybroeck Pierre Dorny Sarah Gabriël |
author_facet |
Kabemba E Mwape Isaac K Phiri Nicolas Praet John B Muma Gideon Zulu Peter Van den Bossche Reginald de Deken Niko Speybroeck Pierre Dorny Sarah Gabriël |
author_sort |
Kabemba E Mwape |
title |
Taenia solium Infections in a rural area of Eastern Zambia-a community based study. |
title_short |
Taenia solium Infections in a rural area of Eastern Zambia-a community based study. |
title_full |
Taenia solium Infections in a rural area of Eastern Zambia-a community based study. |
title_fullStr |
Taenia solium Infections in a rural area of Eastern Zambia-a community based study. |
title_full_unstemmed |
Taenia solium Infections in a rural area of Eastern Zambia-a community based study. |
title_sort |
taenia solium infections in a rural area of eastern zambia-a community based study. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://doi.org/10.1371/journal.pntd.0001594 https://doaj.org/article/922f74ebe81042fe9f63f953b541cc10 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 6, Iss 3, p e1594 (2012) |
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http://europepmc.org/articles/PMC3313923?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001594 https://doaj.org/article/922f74ebe81042fe9f63f953b541cc10 |
op_doi |
https://doi.org/10.1371/journal.pntd.0001594 |
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PLoS Neglected Tropical Diseases |
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6 |
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3 |
container_start_page |
e1594 |
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1766346422135816192 |