Aerotolerant Thiosulfate-Reducing Bacterium Fusibacter sp. Strain WBS Isolated from Littoral Bottom Sediments of the White Sea—Biochemical and Genome Analysis
The strain WBS, an anaerobic, psychro- and halotolerant bacterium belonging to the genus Fusibacter , was isolated from the littoral bottom sediments of the White Sea, Arctic, Russia. Fusibacter bizertensis WBS grew at temperatures between 8 and 32 °C (optimum growth at 18–20 °C), pH between 5.2 and...
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MDPI AG
2023
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Online Access: | https://doi.org/10.3390/microorganisms11071642 https://doaj.org/article/92072d28f79a4c3183aa0d44d053a9a5 |
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ftdoajarticles:oai:doaj.org/article:92072d28f79a4c3183aa0d44d053a9a5 2023-08-20T04:04:50+02:00 Aerotolerant Thiosulfate-Reducing Bacterium Fusibacter sp. Strain WBS Isolated from Littoral Bottom Sediments of the White Sea—Biochemical and Genome Analysis Andrei L. Brioukhanov Vitaly V. Kadnikov Alexey V. Beletsky Alexander S. Savvichev 2023-06-01T00:00:00Z https://doi.org/10.3390/microorganisms11071642 https://doaj.org/article/92072d28f79a4c3183aa0d44d053a9a5 EN eng MDPI AG https://www.mdpi.com/2076-2607/11/7/1642 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms11071642 2076-2607 https://doaj.org/article/92072d28f79a4c3183aa0d44d053a9a5 Microorganisms, Vol 11, Iss 1642, p 1642 (2023) Fusibacter thiosulfate-reducing bacteria antioxidant defense genome sequencing White Sea marine bottom sediments Biology (General) QH301-705.5 article 2023 ftdoajarticles https://doi.org/10.3390/microorganisms11071642 2023-07-30T00:35:07Z The strain WBS, an anaerobic, psychro- and halotolerant bacterium belonging to the genus Fusibacter , was isolated from the littoral bottom sediments of the White Sea, Arctic, Russia. Fusibacter bizertensis WBS grew at temperatures between 8 and 32 °C (optimum growth at 18–20 °C), pH between 5.2 and 8.3 (optimum growth at pH 7.2), and at NaCl concentrations between 0 and 70 g L −1 (optimum growth at 32 g L −1 ). It reduced sulfate, thiosulfate, and elemental sulfur into sulfide, and, probably, the strain is able to disproportionate thiosulfate. The strain also utilized a wide range of substrates as it is a chemoorganotrophic bacterium. Analysis of the sequenced genome revealed genes for all enzymes involved in the Embden–Meyerhof glycolytic pathway as well as genes for the non-oxidative stage of the pentose phosphate pathway. The presence of genes encoding aldehyde dehydrogenases and alcohol dehydrogenases also suggests that, in addition to acetate, alcohols can also be the fermentation products. The strain possessed superoxide dismutase and peroxidase activities and the ability to consume O 2 , which is in full accordance with the presence of corresponding genes of antioxidant defense in the genome. The phylogenetic analysis suggested that the strain WBS is the closest relative of Fusibacter bizertensis LTF Kr01 T (16S rRNA gene sequence similarity 98.78%). Based on biochemical and genomic characteristics, the strain WBS is proposed to represent a novel aero-, halo- and psychrotolerant strain from the genus Fusibacter , isolated for the first time among its members from cold oxygenated marine bottom sediments. Article in Journal/Newspaper Arctic White Sea Directory of Open Access Journals: DOAJ Articles Arctic White Sea Microorganisms 11 7 1642 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Fusibacter thiosulfate-reducing bacteria antioxidant defense genome sequencing White Sea marine bottom sediments Biology (General) QH301-705.5 |
spellingShingle |
Fusibacter thiosulfate-reducing bacteria antioxidant defense genome sequencing White Sea marine bottom sediments Biology (General) QH301-705.5 Andrei L. Brioukhanov Vitaly V. Kadnikov Alexey V. Beletsky Alexander S. Savvichev Aerotolerant Thiosulfate-Reducing Bacterium Fusibacter sp. Strain WBS Isolated from Littoral Bottom Sediments of the White Sea—Biochemical and Genome Analysis |
topic_facet |
Fusibacter thiosulfate-reducing bacteria antioxidant defense genome sequencing White Sea marine bottom sediments Biology (General) QH301-705.5 |
description |
The strain WBS, an anaerobic, psychro- and halotolerant bacterium belonging to the genus Fusibacter , was isolated from the littoral bottom sediments of the White Sea, Arctic, Russia. Fusibacter bizertensis WBS grew at temperatures between 8 and 32 °C (optimum growth at 18–20 °C), pH between 5.2 and 8.3 (optimum growth at pH 7.2), and at NaCl concentrations between 0 and 70 g L −1 (optimum growth at 32 g L −1 ). It reduced sulfate, thiosulfate, and elemental sulfur into sulfide, and, probably, the strain is able to disproportionate thiosulfate. The strain also utilized a wide range of substrates as it is a chemoorganotrophic bacterium. Analysis of the sequenced genome revealed genes for all enzymes involved in the Embden–Meyerhof glycolytic pathway as well as genes for the non-oxidative stage of the pentose phosphate pathway. The presence of genes encoding aldehyde dehydrogenases and alcohol dehydrogenases also suggests that, in addition to acetate, alcohols can also be the fermentation products. The strain possessed superoxide dismutase and peroxidase activities and the ability to consume O 2 , which is in full accordance with the presence of corresponding genes of antioxidant defense in the genome. The phylogenetic analysis suggested that the strain WBS is the closest relative of Fusibacter bizertensis LTF Kr01 T (16S rRNA gene sequence similarity 98.78%). Based on biochemical and genomic characteristics, the strain WBS is proposed to represent a novel aero-, halo- and psychrotolerant strain from the genus Fusibacter , isolated for the first time among its members from cold oxygenated marine bottom sediments. |
format |
Article in Journal/Newspaper |
author |
Andrei L. Brioukhanov Vitaly V. Kadnikov Alexey V. Beletsky Alexander S. Savvichev |
author_facet |
Andrei L. Brioukhanov Vitaly V. Kadnikov Alexey V. Beletsky Alexander S. Savvichev |
author_sort |
Andrei L. Brioukhanov |
title |
Aerotolerant Thiosulfate-Reducing Bacterium Fusibacter sp. Strain WBS Isolated from Littoral Bottom Sediments of the White Sea—Biochemical and Genome Analysis |
title_short |
Aerotolerant Thiosulfate-Reducing Bacterium Fusibacter sp. Strain WBS Isolated from Littoral Bottom Sediments of the White Sea—Biochemical and Genome Analysis |
title_full |
Aerotolerant Thiosulfate-Reducing Bacterium Fusibacter sp. Strain WBS Isolated from Littoral Bottom Sediments of the White Sea—Biochemical and Genome Analysis |
title_fullStr |
Aerotolerant Thiosulfate-Reducing Bacterium Fusibacter sp. Strain WBS Isolated from Littoral Bottom Sediments of the White Sea—Biochemical and Genome Analysis |
title_full_unstemmed |
Aerotolerant Thiosulfate-Reducing Bacterium Fusibacter sp. Strain WBS Isolated from Littoral Bottom Sediments of the White Sea—Biochemical and Genome Analysis |
title_sort |
aerotolerant thiosulfate-reducing bacterium fusibacter sp. strain wbs isolated from littoral bottom sediments of the white sea—biochemical and genome analysis |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/microorganisms11071642 https://doaj.org/article/92072d28f79a4c3183aa0d44d053a9a5 |
geographic |
Arctic White Sea |
geographic_facet |
Arctic White Sea |
genre |
Arctic White Sea |
genre_facet |
Arctic White Sea |
op_source |
Microorganisms, Vol 11, Iss 1642, p 1642 (2023) |
op_relation |
https://www.mdpi.com/2076-2607/11/7/1642 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms11071642 2076-2607 https://doaj.org/article/92072d28f79a4c3183aa0d44d053a9a5 |
op_doi |
https://doi.org/10.3390/microorganisms11071642 |
container_title |
Microorganisms |
container_volume |
11 |
container_issue |
7 |
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1642 |
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1774715256586633216 |