La funcionalidad de los linfocitos TCD8+ durante la tuberculosis pulmonar activa en la infección por VIH

• Main Authors: Alfonzo Miguel, Suárez José, Comegna Mario, Valenzuela Patricia, Hung Alida, Hernández Dimas
• Format: Article in Journal/Newspaper
• Language: English; Spanish
• Published: Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales 2012
• Subjects: VIH; Tuberculosis; linfocitos T CD8+; citocinas; HIV; CD8+ T lymphocytes; cytokines; Arctic medicine. Tropical medicine; RC955-962; Public aspects of medicine; RA1-1270; Arctic
• Online Access: https://doaj.org/article/91f1999946294353aacad73398a8876a

Abstract: The role of CD4+ T-cells in immunity against tuberculosis has been extensively studied. However, the function of CD8+ T-cells in co-infections of HIV-1 and M. tuberculosis (VIH/Mtb) is not well understood. To evaluate the functionality of CD8+ T-cells from HIV+ patients having active TB, several immunological parameters were assayed. Results indicate that both HIV+ groups (with and without active TB), having the same viral load and peripheral blood CD4+ T and amount of lymphocytes in the blood, displayed different alterations in the ex vivo frequency for CD95 and CD127 markers in CD8+ T-cells, naive and memory positive cells, while for the HLA-DR marker, differences were observed in the total cell population. Moreover, CD8+ T- cells from TB-active patients, cultured for 72 h in the presence of PPD, showed a low proliferation rate and a high frequency of induced apoptosis as well as lower production of IFN-y IL-10 and TNF-a cytokines compared to healthy control cells. These results support the hypothesis that CD8+ T-cells lose their functionality against Mtb during an HIV-1 infection, which could be responsible for the activation of TB in VIH/Mtb co-infected patients. Resumen: El papel de las células TCD4+ en la inmunidad contra la tuberculosis ha sido ampliamente estudiado. Sin embargo, la función de las células TCD8+ en la co-infección VIH-1 y M. tuberculosis (VIH/Mtb) no está bien comprendida. Para determinar la funcionalidad de estas células TCD8+ de pacientes VIH+ con TB (tuberculosis) pulmonar activa se usaron diferentes parámetros inmunológicos. Los resultados indican que ambos grupos de pacientes VIH+ (con o sin TB activa) con la misma carga viral y cantidad de linfocitos TCD4+ en sangre, presentaron diferentes alteraciones en la frecuencia ex vivo para las moléculas CD95 y CD127 en células TCD8+ totales, vírgenes y memoria positivas, mientras que para la molécula HLA-DR, las diferencias fueron observadas en la población celular total. Además, las células TCD8+ de los pacientes con TB activa ...