Metabolomic Study of Heterotrophically Grown Chlorella sp. Isolated from Wastewater in Northern Sweden
There are numerous strains of Chlorella with a corresponding variety of metabolic pathways. A strain we previously isolated from wastewater in northern Sweden can grow heterotrophically as well as autotrophically in light and has higher lipid contents under heterotrophic growth conditions. The aims...
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2021
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ftdoajarticles:oai:doaj.org/article:91c3ed40a34144fea851e44c4c3eea1b 2023-05-15T17:44:24+02:00 Metabolomic Study of Heterotrophically Grown Chlorella sp. Isolated from Wastewater in Northern Sweden Jean Claude Nzayisenga Anita Sellstedt 2021-04-01T00:00:00Z https://doi.org/10.3390/molecules26092410 https://doaj.org/article/91c3ed40a34144fea851e44c4c3eea1b EN eng MDPI AG https://www.mdpi.com/1420-3049/26/9/2410 https://doaj.org/toc/1420-3049 doi:10.3390/molecules26092410 1420-3049 https://doaj.org/article/91c3ed40a34144fea851e44c4c3eea1b Molecules, Vol 26, Iss 2410, p 2410 (2021) autotrophy biodiesel Chlorella sp fatty acids heterotrophy metabolomics Organic chemistry QD241-441 article 2021 ftdoajarticles https://doi.org/10.3390/molecules26092410 2022-12-31T10:28:26Z There are numerous strains of Chlorella with a corresponding variety of metabolic pathways. A strain we previously isolated from wastewater in northern Sweden can grow heterotrophically as well as autotrophically in light and has higher lipid contents under heterotrophic growth conditions. The aims of the present study were to characterize metabolic changes associated with the higher lipid contents in order to enhance our understanding of lipid production in microalgae and potentially identify new compounds with utility in sustainable development. Inter alia , the amino acids glutamine and lysine were 7-fold more abundant under heterotrophic conditions, the key metabolic intermediate alpha-ketoglutarate was more abundant under heterotrophic conditions with glucose, and maltose was more abundant under heterotrophic conditions with glycerol than under autotrophic conditions. The metabolite 3-hydroxy-butyric acid, the direct precursor of the biodegradable plastic PHB (poly-3-hydroxy-butyric acid), was also more abundant under heterotrophic conditions. Our metabolomic analysis has provided new insights into the alga’s lipid production pathways and identified metabolites with potential use in sustainable development, such as the production of renewable, biodegradable plastics, cosmetics, and nutraceuticals, with reduced pollution and improvements in both ecological and human health. Article in Journal/Newspaper Northern Sweden Directory of Open Access Journals: DOAJ Articles Molecules 26 9 2410 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
autotrophy biodiesel Chlorella sp fatty acids heterotrophy metabolomics Organic chemistry QD241-441 |
spellingShingle |
autotrophy biodiesel Chlorella sp fatty acids heterotrophy metabolomics Organic chemistry QD241-441 Jean Claude Nzayisenga Anita Sellstedt Metabolomic Study of Heterotrophically Grown Chlorella sp. Isolated from Wastewater in Northern Sweden |
topic_facet |
autotrophy biodiesel Chlorella sp fatty acids heterotrophy metabolomics Organic chemistry QD241-441 |
description |
There are numerous strains of Chlorella with a corresponding variety of metabolic pathways. A strain we previously isolated from wastewater in northern Sweden can grow heterotrophically as well as autotrophically in light and has higher lipid contents under heterotrophic growth conditions. The aims of the present study were to characterize metabolic changes associated with the higher lipid contents in order to enhance our understanding of lipid production in microalgae and potentially identify new compounds with utility in sustainable development. Inter alia , the amino acids glutamine and lysine were 7-fold more abundant under heterotrophic conditions, the key metabolic intermediate alpha-ketoglutarate was more abundant under heterotrophic conditions with glucose, and maltose was more abundant under heterotrophic conditions with glycerol than under autotrophic conditions. The metabolite 3-hydroxy-butyric acid, the direct precursor of the biodegradable plastic PHB (poly-3-hydroxy-butyric acid), was also more abundant under heterotrophic conditions. Our metabolomic analysis has provided new insights into the alga’s lipid production pathways and identified metabolites with potential use in sustainable development, such as the production of renewable, biodegradable plastics, cosmetics, and nutraceuticals, with reduced pollution and improvements in both ecological and human health. |
format |
Article in Journal/Newspaper |
author |
Jean Claude Nzayisenga Anita Sellstedt |
author_facet |
Jean Claude Nzayisenga Anita Sellstedt |
author_sort |
Jean Claude Nzayisenga |
title |
Metabolomic Study of Heterotrophically Grown Chlorella sp. Isolated from Wastewater in Northern Sweden |
title_short |
Metabolomic Study of Heterotrophically Grown Chlorella sp. Isolated from Wastewater in Northern Sweden |
title_full |
Metabolomic Study of Heterotrophically Grown Chlorella sp. Isolated from Wastewater in Northern Sweden |
title_fullStr |
Metabolomic Study of Heterotrophically Grown Chlorella sp. Isolated from Wastewater in Northern Sweden |
title_full_unstemmed |
Metabolomic Study of Heterotrophically Grown Chlorella sp. Isolated from Wastewater in Northern Sweden |
title_sort |
metabolomic study of heterotrophically grown chlorella sp. isolated from wastewater in northern sweden |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/molecules26092410 https://doaj.org/article/91c3ed40a34144fea851e44c4c3eea1b |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_source |
Molecules, Vol 26, Iss 2410, p 2410 (2021) |
op_relation |
https://www.mdpi.com/1420-3049/26/9/2410 https://doaj.org/toc/1420-3049 doi:10.3390/molecules26092410 1420-3049 https://doaj.org/article/91c3ed40a34144fea851e44c4c3eea1b |
op_doi |
https://doi.org/10.3390/molecules26092410 |
container_title |
Molecules |
container_volume |
26 |
container_issue |
9 |
container_start_page |
2410 |
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1766146621779738624 |