Enzymatic Synthesis of Glucose Monodecanoate in a Hydrophobic Deep Eutectic Solvent
Environmentally friendly and biodegradable reaction media are an important part of a sustainable glycolipid production in the transition to green chemistry. Deep eutectic solvents (DESs) are an ecofriendly alternative to organic solvents. So far, only hydrophilic DESs were considered for enzymatic g...
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ftdoajarticles:oai:doaj.org/article:91c3527b0c3143b7ac2fba0feda468ff 2023-05-15T13:41:32+02:00 Enzymatic Synthesis of Glucose Monodecanoate in a Hydrophobic Deep Eutectic Solvent Rebecca Hollenbach Katrin Ochsenreither Christoph Syldatk 2020-06-01T00:00:00Z https://doi.org/10.3390/ijms21124342 https://doaj.org/article/91c3527b0c3143b7ac2fba0feda468ff EN eng MDPI AG https://www.mdpi.com/1422-0067/21/12/4342 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms21124342 1422-0067 1661-6596 https://doaj.org/article/91c3527b0c3143b7ac2fba0feda468ff International Journal of Molecular Sciences, Vol 21, Iss 4342, p 4342 (2020) glycolipid deep eutectic solvents enzymatic synthesis Candida antarctica lipase B polarity Biology (General) QH301-705.5 Chemistry QD1-999 article 2020 ftdoajarticles https://doi.org/10.3390/ijms21124342 2022-12-31T03:36:57Z Environmentally friendly and biodegradable reaction media are an important part of a sustainable glycolipid production in the transition to green chemistry. Deep eutectic solvents (DESs) are an ecofriendly alternative to organic solvents. So far, only hydrophilic DESs were considered for enzymatic glycolipid synthesis. In this study, a hydrophobic DES consisting of (-)-menthol and decanoic acid is presented for the first time as an alternative to hydrophilic DES. The yields in the newly introduced hydrophobic DES are significantly higher than in hydrophilic DESs. Different reaction parameters were investigated to optimize the synthesis further. Twenty milligrams per milliliter iCalB and 0.5 M glucose resulted in the highest initial reaction velocity for the esterification reaction, while the highest initial reaction velocity was achieved with 1.5 M glucose in the transesterification reaction. The enzyme was proven to be reusable for at least five cycles without significant loss of activity. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles International Journal of Molecular Sciences 21 12 4342 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
glycolipid deep eutectic solvents enzymatic synthesis Candida antarctica lipase B polarity Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
glycolipid deep eutectic solvents enzymatic synthesis Candida antarctica lipase B polarity Biology (General) QH301-705.5 Chemistry QD1-999 Rebecca Hollenbach Katrin Ochsenreither Christoph Syldatk Enzymatic Synthesis of Glucose Monodecanoate in a Hydrophobic Deep Eutectic Solvent |
topic_facet |
glycolipid deep eutectic solvents enzymatic synthesis Candida antarctica lipase B polarity Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
Environmentally friendly and biodegradable reaction media are an important part of a sustainable glycolipid production in the transition to green chemistry. Deep eutectic solvents (DESs) are an ecofriendly alternative to organic solvents. So far, only hydrophilic DESs were considered for enzymatic glycolipid synthesis. In this study, a hydrophobic DES consisting of (-)-menthol and decanoic acid is presented for the first time as an alternative to hydrophilic DES. The yields in the newly introduced hydrophobic DES are significantly higher than in hydrophilic DESs. Different reaction parameters were investigated to optimize the synthesis further. Twenty milligrams per milliliter iCalB and 0.5 M glucose resulted in the highest initial reaction velocity for the esterification reaction, while the highest initial reaction velocity was achieved with 1.5 M glucose in the transesterification reaction. The enzyme was proven to be reusable for at least five cycles without significant loss of activity. |
format |
Article in Journal/Newspaper |
author |
Rebecca Hollenbach Katrin Ochsenreither Christoph Syldatk |
author_facet |
Rebecca Hollenbach Katrin Ochsenreither Christoph Syldatk |
author_sort |
Rebecca Hollenbach |
title |
Enzymatic Synthesis of Glucose Monodecanoate in a Hydrophobic Deep Eutectic Solvent |
title_short |
Enzymatic Synthesis of Glucose Monodecanoate in a Hydrophobic Deep Eutectic Solvent |
title_full |
Enzymatic Synthesis of Glucose Monodecanoate in a Hydrophobic Deep Eutectic Solvent |
title_fullStr |
Enzymatic Synthesis of Glucose Monodecanoate in a Hydrophobic Deep Eutectic Solvent |
title_full_unstemmed |
Enzymatic Synthesis of Glucose Monodecanoate in a Hydrophobic Deep Eutectic Solvent |
title_sort |
enzymatic synthesis of glucose monodecanoate in a hydrophobic deep eutectic solvent |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/ijms21124342 https://doaj.org/article/91c3527b0c3143b7ac2fba0feda468ff |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
International Journal of Molecular Sciences, Vol 21, Iss 4342, p 4342 (2020) |
op_relation |
https://www.mdpi.com/1422-0067/21/12/4342 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms21124342 1422-0067 1661-6596 https://doaj.org/article/91c3527b0c3143b7ac2fba0feda468ff |
op_doi |
https://doi.org/10.3390/ijms21124342 |
container_title |
International Journal of Molecular Sciences |
container_volume |
21 |
container_issue |
12 |
container_start_page |
4342 |
_version_ |
1766151822929559552 |