Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis
The Pleuronectiformes order, which includes several commercially-important species, has undergone extensive chromosome evolution. One of these species is Solea senegalensis , a flatfish with 2 n = 42 chromosomes. In this study, a cytogenomics approach and integration with previous maps was applied t...
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ftdoajarticles:oai:doaj.org/article:91a8615041444c3792a9b9248c3342eb 2023-05-15T18:15:51+02:00 Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis Daniel Ramírez María Esther Rodríguez Ismael Cross Alberto Arias-Pérez Manuel Alejandro Merlo Marco Anaya Silvia Portela-Bens Paulino Martínez Francisca Robles Carmelo Ruiz-Rejón Laureana Rebordinos 2022-05-01T00:00:00Z https://doi.org/10.3390/ijms23105353 https://doaj.org/article/91a8615041444c3792a9b9248c3342eb EN eng MDPI AG https://www.mdpi.com/1422-0067/23/10/5353 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms23105353 1422-0067 1661-6596 https://doaj.org/article/91a8615041444c3792a9b9248c3342eb International Journal of Molecular Sciences, Vol 23, Iss 5353, p 5353 (2022) Solea senegalensis pleuronectiformes genetic maps cytogenomics chromosome evolution karyotype Biology (General) QH301-705.5 Chemistry QD1-999 article 2022 ftdoajarticles https://doi.org/10.3390/ijms23105353 2022-12-30T23:54:55Z The Pleuronectiformes order, which includes several commercially-important species, has undergone extensive chromosome evolution. One of these species is Solea senegalensis , a flatfish with 2 n = 42 chromosomes. In this study, a cytogenomics approach and integration with previous maps was applied to characterize the karyotype of the species. Synteny analysis of S. senegalensis was carried out using two flatfish as a reference: Cynoglossus semilaevis and Scophthalmus maximus . Most S. senegalensis chromosomes (or chromosome arms for metacentrics and submetacentrics) showed a one-to-one macrosyntenic pattern with the other two species. In addition, we studied how repetitive sequences could have played a role in the evolution of S. senegalensis bi-armed (3, and 5–9) and acrocentric (11, 12 and 16) chromosomes, which showed the highest rearrangements compared with the reference species. A higher abundance of TEs (Transposable Elements) and other repeated elements was observed adjacent to telomeric regions on chromosomes 3, 7, 9 and 16. However, on chromosome 11, a greater abundance of DNA transposons was detected in interstitial BACs. This chromosome is syntenic with several chromosomes of the other two flatfish species, suggesting rearrangements during its evolution. A similar situation was also found on chromosome 16 (for microsatellites and low complexity sequences), but not for TEs (retroelements and DNA transposons). These differences in the distribution and abundance of repetitive elements in chromosomes that have undergone remodeling processes during the course of evolution also suggest a possible role for simple repeat sequences in rearranged regions. Article in Journal/Newspaper Scophthalmus maximus Directory of Open Access Journals: DOAJ Articles International Journal of Molecular Sciences 23 10 5353 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Solea senegalensis pleuronectiformes genetic maps cytogenomics chromosome evolution karyotype Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
Solea senegalensis pleuronectiformes genetic maps cytogenomics chromosome evolution karyotype Biology (General) QH301-705.5 Chemistry QD1-999 Daniel Ramírez María Esther Rodríguez Ismael Cross Alberto Arias-Pérez Manuel Alejandro Merlo Marco Anaya Silvia Portela-Bens Paulino Martínez Francisca Robles Carmelo Ruiz-Rejón Laureana Rebordinos Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis |
topic_facet |
Solea senegalensis pleuronectiformes genetic maps cytogenomics chromosome evolution karyotype Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
The Pleuronectiformes order, which includes several commercially-important species, has undergone extensive chromosome evolution. One of these species is Solea senegalensis , a flatfish with 2 n = 42 chromosomes. In this study, a cytogenomics approach and integration with previous maps was applied to characterize the karyotype of the species. Synteny analysis of S. senegalensis was carried out using two flatfish as a reference: Cynoglossus semilaevis and Scophthalmus maximus . Most S. senegalensis chromosomes (or chromosome arms for metacentrics and submetacentrics) showed a one-to-one macrosyntenic pattern with the other two species. In addition, we studied how repetitive sequences could have played a role in the evolution of S. senegalensis bi-armed (3, and 5–9) and acrocentric (11, 12 and 16) chromosomes, which showed the highest rearrangements compared with the reference species. A higher abundance of TEs (Transposable Elements) and other repeated elements was observed adjacent to telomeric regions on chromosomes 3, 7, 9 and 16. However, on chromosome 11, a greater abundance of DNA transposons was detected in interstitial BACs. This chromosome is syntenic with several chromosomes of the other two flatfish species, suggesting rearrangements during its evolution. A similar situation was also found on chromosome 16 (for microsatellites and low complexity sequences), but not for TEs (retroelements and DNA transposons). These differences in the distribution and abundance of repetitive elements in chromosomes that have undergone remodeling processes during the course of evolution also suggest a possible role for simple repeat sequences in rearranged regions. |
format |
Article in Journal/Newspaper |
author |
Daniel Ramírez María Esther Rodríguez Ismael Cross Alberto Arias-Pérez Manuel Alejandro Merlo Marco Anaya Silvia Portela-Bens Paulino Martínez Francisca Robles Carmelo Ruiz-Rejón Laureana Rebordinos |
author_facet |
Daniel Ramírez María Esther Rodríguez Ismael Cross Alberto Arias-Pérez Manuel Alejandro Merlo Marco Anaya Silvia Portela-Bens Paulino Martínez Francisca Robles Carmelo Ruiz-Rejón Laureana Rebordinos |
author_sort |
Daniel Ramírez |
title |
Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis |
title_short |
Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis |
title_full |
Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis |
title_fullStr |
Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis |
title_full_unstemmed |
Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis |
title_sort |
integration of maps enables a cytogenomics analysis of the complete karyotype in solea senegalensis |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/ijms23105353 https://doaj.org/article/91a8615041444c3792a9b9248c3342eb |
genre |
Scophthalmus maximus |
genre_facet |
Scophthalmus maximus |
op_source |
International Journal of Molecular Sciences, Vol 23, Iss 5353, p 5353 (2022) |
op_relation |
https://www.mdpi.com/1422-0067/23/10/5353 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms23105353 1422-0067 1661-6596 https://doaj.org/article/91a8615041444c3792a9b9248c3342eb |
op_doi |
https://doi.org/10.3390/ijms23105353 |
container_title |
International Journal of Molecular Sciences |
container_volume |
23 |
container_issue |
10 |
container_start_page |
5353 |
_version_ |
1766189083308064768 |