Biofilm Produced In Vitro by Piscirickettsia salmonis Generates Differential Cytotoxicity Levels and Expression Patterns of Immune Genes in the Atlantic Salmon Cell Line SHK-1
Piscirickettsia salmonis is the causative agent of Piscirickettsiosis, an infectious disease with a high economic impact on the Chilean salmonid aquaculture industry. This bacterium produces biofilm as a potential resistance and persistence strategy against stressful environmental stimuli. However,...
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ftdoajarticles:oai:doaj.org/article:916a6a15fb3d449999442257f4ed575b 2023-05-15T15:31:32+02:00 Biofilm Produced In Vitro by Piscirickettsia salmonis Generates Differential Cytotoxicity Levels and Expression Patterns of Immune Genes in the Atlantic Salmon Cell Line SHK-1 Natacha Santibañez Matías Vega Tatiana Pérez Alejandro Yáñez Roxana González-Stegmaier Jaime Figueroa Ricardo Enríquez Cristian Oliver Alex Romero 2020-10-01T00:00:00Z https://doi.org/10.3390/microorganisms8101609 https://doaj.org/article/916a6a15fb3d449999442257f4ed575b EN eng MDPI AG https://www.mdpi.com/2076-2607/8/10/1609 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms8101609 2076-2607 https://doaj.org/article/916a6a15fb3d449999442257f4ed575b Microorganisms, Vol 8, Iss 1609, p 1609 (2020) Piscirickettsia salmonis SRS biofilm gene expression bacterial virulence Biology (General) QH301-705.5 article 2020 ftdoajarticles https://doi.org/10.3390/microorganisms8101609 2022-12-31T13:54:38Z Piscirickettsia salmonis is the causative agent of Piscirickettsiosis, an infectious disease with a high economic impact on the Chilean salmonid aquaculture industry. This bacterium produces biofilm as a potential resistance and persistence strategy against stressful environmental stimuli. However, the in vitro culture conditions that modulate biofilm formation as well as the effect of sessile bacteria on virulence and immune gene expression in host cells have not been described for P. salmonis . Therefore, this study aimed to analyze the biofilm formation by P. salmonis isolates under several NaCl and iron concentrations and to evaluate the virulence of planktonic and sessile bacteria, together with the immune gene expression induced by these bacterial conditions in an Atlantic salmon macrophage cell line. Our results showed that NaCl and Fe significantly increased biofilm production in the LF-89 type strain and EM-90-like isolates. Additionally, the planktonic EM-90 isolate and sessile LF-89 generated the highest virulence levels, associated with differential expression of il-1β , il-8 , nf-κb , and iκb -α genes in SHK-1 cells. These results suggest that there is no single virulence pattern or gene expression profile induced by the planktonic or sessile condition of P. salmonis , which are dependent on each strain and bacterial condition used. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles Microorganisms 8 10 1609 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Piscirickettsia salmonis SRS biofilm gene expression bacterial virulence Biology (General) QH301-705.5 |
spellingShingle |
Piscirickettsia salmonis SRS biofilm gene expression bacterial virulence Biology (General) QH301-705.5 Natacha Santibañez Matías Vega Tatiana Pérez Alejandro Yáñez Roxana González-Stegmaier Jaime Figueroa Ricardo Enríquez Cristian Oliver Alex Romero Biofilm Produced In Vitro by Piscirickettsia salmonis Generates Differential Cytotoxicity Levels and Expression Patterns of Immune Genes in the Atlantic Salmon Cell Line SHK-1 |
topic_facet |
Piscirickettsia salmonis SRS biofilm gene expression bacterial virulence Biology (General) QH301-705.5 |
description |
Piscirickettsia salmonis is the causative agent of Piscirickettsiosis, an infectious disease with a high economic impact on the Chilean salmonid aquaculture industry. This bacterium produces biofilm as a potential resistance and persistence strategy against stressful environmental stimuli. However, the in vitro culture conditions that modulate biofilm formation as well as the effect of sessile bacteria on virulence and immune gene expression in host cells have not been described for P. salmonis . Therefore, this study aimed to analyze the biofilm formation by P. salmonis isolates under several NaCl and iron concentrations and to evaluate the virulence of planktonic and sessile bacteria, together with the immune gene expression induced by these bacterial conditions in an Atlantic salmon macrophage cell line. Our results showed that NaCl and Fe significantly increased biofilm production in the LF-89 type strain and EM-90-like isolates. Additionally, the planktonic EM-90 isolate and sessile LF-89 generated the highest virulence levels, associated with differential expression of il-1β , il-8 , nf-κb , and iκb -α genes in SHK-1 cells. These results suggest that there is no single virulence pattern or gene expression profile induced by the planktonic or sessile condition of P. salmonis , which are dependent on each strain and bacterial condition used. |
format |
Article in Journal/Newspaper |
author |
Natacha Santibañez Matías Vega Tatiana Pérez Alejandro Yáñez Roxana González-Stegmaier Jaime Figueroa Ricardo Enríquez Cristian Oliver Alex Romero |
author_facet |
Natacha Santibañez Matías Vega Tatiana Pérez Alejandro Yáñez Roxana González-Stegmaier Jaime Figueroa Ricardo Enríquez Cristian Oliver Alex Romero |
author_sort |
Natacha Santibañez |
title |
Biofilm Produced In Vitro by Piscirickettsia salmonis Generates Differential Cytotoxicity Levels and Expression Patterns of Immune Genes in the Atlantic Salmon Cell Line SHK-1 |
title_short |
Biofilm Produced In Vitro by Piscirickettsia salmonis Generates Differential Cytotoxicity Levels and Expression Patterns of Immune Genes in the Atlantic Salmon Cell Line SHK-1 |
title_full |
Biofilm Produced In Vitro by Piscirickettsia salmonis Generates Differential Cytotoxicity Levels and Expression Patterns of Immune Genes in the Atlantic Salmon Cell Line SHK-1 |
title_fullStr |
Biofilm Produced In Vitro by Piscirickettsia salmonis Generates Differential Cytotoxicity Levels and Expression Patterns of Immune Genes in the Atlantic Salmon Cell Line SHK-1 |
title_full_unstemmed |
Biofilm Produced In Vitro by Piscirickettsia salmonis Generates Differential Cytotoxicity Levels and Expression Patterns of Immune Genes in the Atlantic Salmon Cell Line SHK-1 |
title_sort |
biofilm produced in vitro by piscirickettsia salmonis generates differential cytotoxicity levels and expression patterns of immune genes in the atlantic salmon cell line shk-1 |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/microorganisms8101609 https://doaj.org/article/916a6a15fb3d449999442257f4ed575b |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Microorganisms, Vol 8, Iss 1609, p 1609 (2020) |
op_relation |
https://www.mdpi.com/2076-2607/8/10/1609 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms8101609 2076-2607 https://doaj.org/article/916a6a15fb3d449999442257f4ed575b |
op_doi |
https://doi.org/10.3390/microorganisms8101609 |
container_title |
Microorganisms |
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8 |
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10 |
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1609 |
_version_ |
1766362057705259008 |