Control of Aedes mosquito populations using recombinant microalgae expressing short hairpin RNAs and their effect on plankton.
New biocontrol strategies are urgently needed to combat vector-borne infectious diseases. This study presents a low-cost method to produce a potential mosquito insecticide that utilizes the microalgae released into suburban water sources to control mosquito populations. Chlorella microalgae are ubiq...
Published in: | PLOS Neglected Tropical Diseases |
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Online Access: | https://doi.org/10.1371/journal.pntd.0011109 https://doaj.org/article/9151c4b5fabc46d0bba8bbf79a35d04d |
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ftdoajarticles:oai:doaj.org/article:9151c4b5fabc46d0bba8bbf79a35d04d 2023-05-15T15:07:17+02:00 Control of Aedes mosquito populations using recombinant microalgae expressing short hairpin RNAs and their effect on plankton. Xiaowen Fei Sha Xiao Xiaodan Huang Zhijie Li Xinghan Li Changhao He Yajun Li Xiuxia Zhang Xiaodong Deng 2023-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0011109 https://doaj.org/article/9151c4b5fabc46d0bba8bbf79a35d04d EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0011109 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011109 https://doaj.org/article/9151c4b5fabc46d0bba8bbf79a35d04d PLoS Neglected Tropical Diseases, Vol 17, Iss 1, p e0011109 (2023) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2023 ftdoajarticles https://doi.org/10.1371/journal.pntd.0011109 2023-03-05T01:33:15Z New biocontrol strategies are urgently needed to combat vector-borne infectious diseases. This study presents a low-cost method to produce a potential mosquito insecticide that utilizes the microalgae released into suburban water sources to control mosquito populations. Chlorella microalgae are ubiquitous in local waters, which were chosen as the host for genetic transfection. This species facilitated the recombinant algae to adapt to the prevailing environmental conditions with rapid growth and high relative abundance. The procedure involved microalgae RNAi-based insecticides developed using short hairpin RNAs targeting the Aedes aegypti chitin synthase A (chsa) gene in Chlorella. These insecticides effectively silenced the chsa gene, inhibiting Aedes metamorphosis in the laboratory and simulated-field trials. This study explored the impact of recombinant microalgae on the phytoplankton and zooplankton in suburban waters. High-throughput sequencing revealed that rapid reproduction of recombinant Chlorella indirectly caused the disappearance of some phytoplankton and reduced the protozoan species. This study demonstrated that a recombinant microalgae-based insecticide could effectively reduce the population of Aedes mosquitoes in the laboratory and simulated field trials. However, the impact of this technology on the environment and ecology requires further investigation. Article in Journal/Newspaper Arctic Phytoplankton Zooplankton Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 17 1 e0011109 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Xiaowen Fei Sha Xiao Xiaodan Huang Zhijie Li Xinghan Li Changhao He Yajun Li Xiuxia Zhang Xiaodong Deng Control of Aedes mosquito populations using recombinant microalgae expressing short hairpin RNAs and their effect on plankton. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
New biocontrol strategies are urgently needed to combat vector-borne infectious diseases. This study presents a low-cost method to produce a potential mosquito insecticide that utilizes the microalgae released into suburban water sources to control mosquito populations. Chlorella microalgae are ubiquitous in local waters, which were chosen as the host for genetic transfection. This species facilitated the recombinant algae to adapt to the prevailing environmental conditions with rapid growth and high relative abundance. The procedure involved microalgae RNAi-based insecticides developed using short hairpin RNAs targeting the Aedes aegypti chitin synthase A (chsa) gene in Chlorella. These insecticides effectively silenced the chsa gene, inhibiting Aedes metamorphosis in the laboratory and simulated-field trials. This study explored the impact of recombinant microalgae on the phytoplankton and zooplankton in suburban waters. High-throughput sequencing revealed that rapid reproduction of recombinant Chlorella indirectly caused the disappearance of some phytoplankton and reduced the protozoan species. This study demonstrated that a recombinant microalgae-based insecticide could effectively reduce the population of Aedes mosquitoes in the laboratory and simulated field trials. However, the impact of this technology on the environment and ecology requires further investigation. |
format |
Article in Journal/Newspaper |
author |
Xiaowen Fei Sha Xiao Xiaodan Huang Zhijie Li Xinghan Li Changhao He Yajun Li Xiuxia Zhang Xiaodong Deng |
author_facet |
Xiaowen Fei Sha Xiao Xiaodan Huang Zhijie Li Xinghan Li Changhao He Yajun Li Xiuxia Zhang Xiaodong Deng |
author_sort |
Xiaowen Fei |
title |
Control of Aedes mosquito populations using recombinant microalgae expressing short hairpin RNAs and their effect on plankton. |
title_short |
Control of Aedes mosquito populations using recombinant microalgae expressing short hairpin RNAs and their effect on plankton. |
title_full |
Control of Aedes mosquito populations using recombinant microalgae expressing short hairpin RNAs and their effect on plankton. |
title_fullStr |
Control of Aedes mosquito populations using recombinant microalgae expressing short hairpin RNAs and their effect on plankton. |
title_full_unstemmed |
Control of Aedes mosquito populations using recombinant microalgae expressing short hairpin RNAs and their effect on plankton. |
title_sort |
control of aedes mosquito populations using recombinant microalgae expressing short hairpin rnas and their effect on plankton. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
https://doi.org/10.1371/journal.pntd.0011109 https://doaj.org/article/9151c4b5fabc46d0bba8bbf79a35d04d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Phytoplankton Zooplankton |
genre_facet |
Arctic Phytoplankton Zooplankton |
op_source |
PLoS Neglected Tropical Diseases, Vol 17, Iss 1, p e0011109 (2023) |
op_relation |
https://doi.org/10.1371/journal.pntd.0011109 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011109 https://doaj.org/article/9151c4b5fabc46d0bba8bbf79a35d04d |
op_doi |
https://doi.org/10.1371/journal.pntd.0011109 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
17 |
container_issue |
1 |
container_start_page |
e0011109 |
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1766338820797628416 |