Infection with Usutu virus induces an autophagic response in mammalian cells.
Usutu virus (USUV) is an African mosquito-borne flavivirus closely related to West Nile virus and Japanese encephalitis virus, which host range includes mainly mosquitoes and birds, although infections in humans have been also documented, thus warning about USUV as a potential health threat. Circula...
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ftdoajarticles:oai:doaj.org/article:914129de812b455abd30d668c2ff0c43 2023-05-15T15:12:38+02:00 Infection with Usutu virus induces an autophagic response in mammalian cells. Ana-Belén Blázquez Estela Escribano-Romero Teresa Merino-Ramos Juan-Carlos Saiz Miguel A Martín-Acebes 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002509 https://doaj.org/article/914129de812b455abd30d668c2ff0c43 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3812092?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002509 https://doaj.org/article/914129de812b455abd30d668c2ff0c43 PLoS Neglected Tropical Diseases, Vol 7, Iss 10, p e2509 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002509 2022-12-31T12:07:08Z Usutu virus (USUV) is an African mosquito-borne flavivirus closely related to West Nile virus and Japanese encephalitis virus, which host range includes mainly mosquitoes and birds, although infections in humans have been also documented, thus warning about USUV as a potential health threat. Circulation of USUV in Africa was documented more than 50 years ago, but it was not until the last decade that it emerged in Europe causing episodes of avian mortality and some human severe cases. Since autophagy is a cellular pathway that can play important roles on different aspects of viral infections and pathogenesis, the possible implication of this pathway in USUV infection has been examined using Vero cells and two viral strains of different origin. USUV infection induced the unfolded protein response, revealed by the splicing of Xbp-1 mRNA. Infection with USUV also stimulated the autophagic process, which was demonstrated by an increase in the cytoplasmic aggregation of microtubule-associated protein 1 light chain 3 (LC3), a marker of autophagosome formation. In addition to this, an increase in the lipidated form of LC3, that is associated with autophagosome formation, was noticed following infection. Pharmacological modulation of the autophagic pathway with the inductor of autophagy rapamycin resulted in an increase in virus yield. On the other hand, treatment with 3-methyladenine or wortmannin, two distinct inhibitors of phosphatidylinositol 3-kinases involved in autophagy, resulted in a decrease in virus yield. These results indicate that USUV virus infection upregulates the cellular autophagic pathway and that drugs that target this pathway can modulate the infection of this virus, thus identifying a potential druggable pathway in USUV-infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 7 10 e2509 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ana-Belén Blázquez Estela Escribano-Romero Teresa Merino-Ramos Juan-Carlos Saiz Miguel A Martín-Acebes Infection with Usutu virus induces an autophagic response in mammalian cells. |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Usutu virus (USUV) is an African mosquito-borne flavivirus closely related to West Nile virus and Japanese encephalitis virus, which host range includes mainly mosquitoes and birds, although infections in humans have been also documented, thus warning about USUV as a potential health threat. Circulation of USUV in Africa was documented more than 50 years ago, but it was not until the last decade that it emerged in Europe causing episodes of avian mortality and some human severe cases. Since autophagy is a cellular pathway that can play important roles on different aspects of viral infections and pathogenesis, the possible implication of this pathway in USUV infection has been examined using Vero cells and two viral strains of different origin. USUV infection induced the unfolded protein response, revealed by the splicing of Xbp-1 mRNA. Infection with USUV also stimulated the autophagic process, which was demonstrated by an increase in the cytoplasmic aggregation of microtubule-associated protein 1 light chain 3 (LC3), a marker of autophagosome formation. In addition to this, an increase in the lipidated form of LC3, that is associated with autophagosome formation, was noticed following infection. Pharmacological modulation of the autophagic pathway with the inductor of autophagy rapamycin resulted in an increase in virus yield. On the other hand, treatment with 3-methyladenine or wortmannin, two distinct inhibitors of phosphatidylinositol 3-kinases involved in autophagy, resulted in a decrease in virus yield. These results indicate that USUV virus infection upregulates the cellular autophagic pathway and that drugs that target this pathway can modulate the infection of this virus, thus identifying a potential druggable pathway in USUV-infection. |
format |
Article in Journal/Newspaper |
author |
Ana-Belén Blázquez Estela Escribano-Romero Teresa Merino-Ramos Juan-Carlos Saiz Miguel A Martín-Acebes |
author_facet |
Ana-Belén Blázquez Estela Escribano-Romero Teresa Merino-Ramos Juan-Carlos Saiz Miguel A Martín-Acebes |
author_sort |
Ana-Belén Blázquez |
title |
Infection with Usutu virus induces an autophagic response in mammalian cells. |
title_short |
Infection with Usutu virus induces an autophagic response in mammalian cells. |
title_full |
Infection with Usutu virus induces an autophagic response in mammalian cells. |
title_fullStr |
Infection with Usutu virus induces an autophagic response in mammalian cells. |
title_full_unstemmed |
Infection with Usutu virus induces an autophagic response in mammalian cells. |
title_sort |
infection with usutu virus induces an autophagic response in mammalian cells. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0002509 https://doaj.org/article/914129de812b455abd30d668c2ff0c43 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 10, p e2509 (2013) |
op_relation |
http://europepmc.org/articles/PMC3812092?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002509 https://doaj.org/article/914129de812b455abd30d668c2ff0c43 |
op_doi |
https://doi.org/10.1371/journal.pntd.0002509 |
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PLoS Neglected Tropical Diseases |
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7 |
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10 |
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e2509 |
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