Use of a novel antigen expressing system to study the Salmonella enterica serovar Typhi protein recognition by T cells.
Salmonella enterica serovar Typhi (S. Typhi), the causative agent of the typhoid fever, is a pathogen of great public health importance. Typhoid vaccines have the potential to be cost-effective measures towards combating this disease, yet the antigens triggering host protective immune responses are...
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ftdoajarticles:oai:doaj.org/article:912e6ffd1b154a7db1f7b073f966437a 2023-05-15T15:12:03+02:00 Use of a novel antigen expressing system to study the Salmonella enterica serovar Typhi protein recognition by T cells. Rosângela Salerno-Gonçalves Hervé Tettelin David Lou Stephanie Steiner Tasmia Rezwanul Qin Guo William D Picking Vishvanath Nene Marcelo B Sztein 2017-09-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005912 https://doaj.org/article/912e6ffd1b154a7db1f7b073f966437a EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5600385?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005912 https://doaj.org/article/912e6ffd1b154a7db1f7b073f966437a PLoS Neglected Tropical Diseases, Vol 11, Iss 9, p e0005912 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005912 2022-12-31T03:53:45Z Salmonella enterica serovar Typhi (S. Typhi), the causative agent of the typhoid fever, is a pathogen of great public health importance. Typhoid vaccines have the potential to be cost-effective measures towards combating this disease, yet the antigens triggering host protective immune responses are largely unknown. Given the key role of cellular-mediated immunity in S. Typhi protection, it is crucial to identify S. Typhi proteins involved in T-cell responses. Here, cells from individuals immunized with Ty21a typhoid vaccine were collected before and after immunization and used as effectors. We also used an innovative antigen expressing system based on the infection of B-cells with recombinant Escherichia coli (E. coli) expressing one of four S. Typhi gene products (i.e., SifA, OmpC, FliC, GroEL) as targets. Using flow cytometry, we found that the pattern of response to specific S. Typhi proteins was variable. Some individuals responded to all four proteins while others responded to only one or two proteins. We next evaluated whether T-cells responding to recombinant E. coli also possess the ability to respond to purified proteins. We observed that CD4+ cell responses, but not CD8+ cell responses, to recombinant E. coli were significantly associated with the responses to purified proteins. Thus, our results demonstrate the feasibility of using an E. coli expressing system to uncover the antigen specificity of T-cells and highlight its applicability to vaccine studies. These results also emphasize the importance of selecting the stimuli appropriately when evaluating CD4+ and CD8+ cell responses. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 9 e0005912 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Rosângela Salerno-Gonçalves Hervé Tettelin David Lou Stephanie Steiner Tasmia Rezwanul Qin Guo William D Picking Vishvanath Nene Marcelo B Sztein Use of a novel antigen expressing system to study the Salmonella enterica serovar Typhi protein recognition by T cells. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Salmonella enterica serovar Typhi (S. Typhi), the causative agent of the typhoid fever, is a pathogen of great public health importance. Typhoid vaccines have the potential to be cost-effective measures towards combating this disease, yet the antigens triggering host protective immune responses are largely unknown. Given the key role of cellular-mediated immunity in S. Typhi protection, it is crucial to identify S. Typhi proteins involved in T-cell responses. Here, cells from individuals immunized with Ty21a typhoid vaccine were collected before and after immunization and used as effectors. We also used an innovative antigen expressing system based on the infection of B-cells with recombinant Escherichia coli (E. coli) expressing one of four S. Typhi gene products (i.e., SifA, OmpC, FliC, GroEL) as targets. Using flow cytometry, we found that the pattern of response to specific S. Typhi proteins was variable. Some individuals responded to all four proteins while others responded to only one or two proteins. We next evaluated whether T-cells responding to recombinant E. coli also possess the ability to respond to purified proteins. We observed that CD4+ cell responses, but not CD8+ cell responses, to recombinant E. coli were significantly associated with the responses to purified proteins. Thus, our results demonstrate the feasibility of using an E. coli expressing system to uncover the antigen specificity of T-cells and highlight its applicability to vaccine studies. These results also emphasize the importance of selecting the stimuli appropriately when evaluating CD4+ and CD8+ cell responses. |
format |
Article in Journal/Newspaper |
author |
Rosângela Salerno-Gonçalves Hervé Tettelin David Lou Stephanie Steiner Tasmia Rezwanul Qin Guo William D Picking Vishvanath Nene Marcelo B Sztein |
author_facet |
Rosângela Salerno-Gonçalves Hervé Tettelin David Lou Stephanie Steiner Tasmia Rezwanul Qin Guo William D Picking Vishvanath Nene Marcelo B Sztein |
author_sort |
Rosângela Salerno-Gonçalves |
title |
Use of a novel antigen expressing system to study the Salmonella enterica serovar Typhi protein recognition by T cells. |
title_short |
Use of a novel antigen expressing system to study the Salmonella enterica serovar Typhi protein recognition by T cells. |
title_full |
Use of a novel antigen expressing system to study the Salmonella enterica serovar Typhi protein recognition by T cells. |
title_fullStr |
Use of a novel antigen expressing system to study the Salmonella enterica serovar Typhi protein recognition by T cells. |
title_full_unstemmed |
Use of a novel antigen expressing system to study the Salmonella enterica serovar Typhi protein recognition by T cells. |
title_sort |
use of a novel antigen expressing system to study the salmonella enterica serovar typhi protein recognition by t cells. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005912 https://doaj.org/article/912e6ffd1b154a7db1f7b073f966437a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 9, p e0005912 (2017) |
op_relation |
http://europepmc.org/articles/PMC5600385?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005912 https://doaj.org/article/912e6ffd1b154a7db1f7b073f966437a |
op_doi |
https://doi.org/10.1371/journal.pntd.0005912 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
9 |
container_start_page |
e0005912 |
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1766342794787422208 |