Comparative analysis of field-isolate and monkey-adapted Plasmodium vivax genomes.
Significant insights into the biology of Plasmodium vivax have been gained from the ability to successfully adapt human infections to non-human primates. P. vivax strains grown in monkeys serve as a renewable source of parasites for in vitro and ex vivo experimental studies and functional assays, or...
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ftdoajarticles:oai:doaj.org/article:90e6d1df00c74574a764d49020be4cac 2023-05-15T15:09:32+02:00 Comparative analysis of field-isolate and monkey-adapted Plasmodium vivax genomes. Ernest R Chan John W Barnwell Peter A Zimmerman David Serre 2015-03-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003566 https://doaj.org/article/90e6d1df00c74574a764d49020be4cac EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4358935?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003566 https://doaj.org/article/90e6d1df00c74574a764d49020be4cac PLoS Neglected Tropical Diseases, Vol 9, Iss 3, p e0003566 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003566 2022-12-31T10:38:05Z Significant insights into the biology of Plasmodium vivax have been gained from the ability to successfully adapt human infections to non-human primates. P. vivax strains grown in monkeys serve as a renewable source of parasites for in vitro and ex vivo experimental studies and functional assays, or for studying in vivo the relapse characteristics, mosquito species compatibilities, drug susceptibility profiles or immune responses towards potential vaccine candidates. Despite the importance of these studies, little is known as to how adaptation to a different host species may influence the genome of P. vivax. In addition, it is unclear whether these monkey-adapted strains consist of a single clonal population of parasites or if they retain the multiclonal complexity commonly observed in field isolates. Here we compare the genome sequences of seven P. vivax strains adapted to New World monkeys with those of six human clinical isolates collected directly in the field. We show that the adaptation of P. vivax parasites to monkey hosts, and their subsequent propagation, did not result in significant modifications of their genome sequence and that these monkey-adapted strains recapitulate the genomic diversity of field isolates. Our analyses also reveal that these strains are not always genetically homogeneous and should be analyzed cautiously. Overall, our study provides a framework to better leverage this important research material and fully utilize this resource for improving our understanding of P. vivax biology. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 9 3 e0003566 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ernest R Chan John W Barnwell Peter A Zimmerman David Serre Comparative analysis of field-isolate and monkey-adapted Plasmodium vivax genomes. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Significant insights into the biology of Plasmodium vivax have been gained from the ability to successfully adapt human infections to non-human primates. P. vivax strains grown in monkeys serve as a renewable source of parasites for in vitro and ex vivo experimental studies and functional assays, or for studying in vivo the relapse characteristics, mosquito species compatibilities, drug susceptibility profiles or immune responses towards potential vaccine candidates. Despite the importance of these studies, little is known as to how adaptation to a different host species may influence the genome of P. vivax. In addition, it is unclear whether these monkey-adapted strains consist of a single clonal population of parasites or if they retain the multiclonal complexity commonly observed in field isolates. Here we compare the genome sequences of seven P. vivax strains adapted to New World monkeys with those of six human clinical isolates collected directly in the field. We show that the adaptation of P. vivax parasites to monkey hosts, and their subsequent propagation, did not result in significant modifications of their genome sequence and that these monkey-adapted strains recapitulate the genomic diversity of field isolates. Our analyses also reveal that these strains are not always genetically homogeneous and should be analyzed cautiously. Overall, our study provides a framework to better leverage this important research material and fully utilize this resource for improving our understanding of P. vivax biology. |
format |
Article in Journal/Newspaper |
author |
Ernest R Chan John W Barnwell Peter A Zimmerman David Serre |
author_facet |
Ernest R Chan John W Barnwell Peter A Zimmerman David Serre |
author_sort |
Ernest R Chan |
title |
Comparative analysis of field-isolate and monkey-adapted Plasmodium vivax genomes. |
title_short |
Comparative analysis of field-isolate and monkey-adapted Plasmodium vivax genomes. |
title_full |
Comparative analysis of field-isolate and monkey-adapted Plasmodium vivax genomes. |
title_fullStr |
Comparative analysis of field-isolate and monkey-adapted Plasmodium vivax genomes. |
title_full_unstemmed |
Comparative analysis of field-isolate and monkey-adapted Plasmodium vivax genomes. |
title_sort |
comparative analysis of field-isolate and monkey-adapted plasmodium vivax genomes. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pntd.0003566 https://doaj.org/article/90e6d1df00c74574a764d49020be4cac |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 9, Iss 3, p e0003566 (2015) |
op_relation |
http://europepmc.org/articles/PMC4358935?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003566 https://doaj.org/article/90e6d1df00c74574a764d49020be4cac |
op_doi |
https://doi.org/10.1371/journal.pntd.0003566 |
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PLOS Neglected Tropical Diseases |
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9 |
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3 |
container_start_page |
e0003566 |
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