Role of aminoglycoside-modifying enzymes and 16S rRNA methylase (ArmA) in resistance of Acinetobacter baumannii clinical isolates against aminoglycosides
Abstract INTRODUCTION: This study aimed to determine the role of genes encoding aminoglycoside-modifying enzymes (AMEs) and 16S rRNA methylase (ArmA) in Acinetobacter baumannii clinical isolates. METHODS: We collected 100 clinical isolates of A. baumannii and identified and confirmed them using micr...
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Sociedade Brasileira de Medicina Tropical (SBMT)
2021
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ftdoajarticles:oai:doaj.org/article:90c8420ca3de4c46b6b0e2711e0ca59d 2023-05-15T15:12:06+02:00 Role of aminoglycoside-modifying enzymes and 16S rRNA methylase (ArmA) in resistance of Acinetobacter baumannii clinical isolates against aminoglycosides Maryam Asadi Jouybari Mohammad Ahanjan Bahman Mirzaei Hamid Reza Goli 2021-01-01T00:00:00Z https://doi.org/10.1590/0037-8682-0599-2020 https://doaj.org/article/90c8420ca3de4c46b6b0e2711e0ca59d EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822021000100305&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0599-2020 https://doaj.org/article/90c8420ca3de4c46b6b0e2711e0ca59d Revista da Sociedade Brasileira de Medicina Tropical, Vol 54 (2021) Acinetobacter baumannii Aminoglycoside-modifying enzymes ArmA Aminoglycoside resistance Arctic medicine. Tropical medicine RC955-962 article 2021 ftdoajarticles https://doi.org/10.1590/0037-8682-0599-2020 2022-12-31T02:52:39Z Abstract INTRODUCTION: This study aimed to determine the role of genes encoding aminoglycoside-modifying enzymes (AMEs) and 16S rRNA methylase (ArmA) in Acinetobacter baumannii clinical isolates. METHODS: We collected 100 clinical isolates of A. baumannii and identified and confirmed them using microbiological tests and assessment of the OXA-51 gene. Antibiotic susceptibility testing was carried out using disk agar diffusion and micro-broth dilution methods. The presence of AME genes and ArmA was detected by PCR and multiplex PCR. RESULTS: The most and least effective antibiotics in this study were netilmicin and ciprofloxacin with 68% and 100% resistance rates, respectively. According to the minimum inhibitory concentration test, 94% of the isolates were resistant to gentamicin, tobramycin, and streptomycin, while the highest susceptibility (20%) was observed against netilmicin. The proportion of strains harboring the aminoglycoside resistance genes was as follows: APH(3′)-VIa (aphA6) (77%), ANT(2”)-Ia (aadB) (73%), ANT(3”)-Ia (aadA1) (33%), AAC(6′)-Ib (aacA4) (33%), ArmA (22%), and AAC(3)-IIa (aacC2) (19%). Among the 22 gene profiles detected in this study, the most prevalent profiles included APH(3′)-VIa + ANT(2”)-Ia (39 isolates, 100% of which were kanamycin-resistant), and AAC(3)-IIa + AAC(6′)-Ib + ANT(3”)-Ia + APH(3′)-VIa + ANT(2”)-Ia (14 isolates, all of which were resistant to gentamicin, kanamycin, and streptomycin). CONCLUSIONS: High minimum inhibitory concentration of aminoglycosides in isolates with the simultaneous presence of AME- and ArmA-encoding genes indicated the importance of these genes in resistance to aminoglycosides. However, control of their spread could be effective in the treatment of infections caused by A. baumannii. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 54 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Acinetobacter baumannii Aminoglycoside-modifying enzymes ArmA Aminoglycoside resistance Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Acinetobacter baumannii Aminoglycoside-modifying enzymes ArmA Aminoglycoside resistance Arctic medicine. Tropical medicine RC955-962 Maryam Asadi Jouybari Mohammad Ahanjan Bahman Mirzaei Hamid Reza Goli Role of aminoglycoside-modifying enzymes and 16S rRNA methylase (ArmA) in resistance of Acinetobacter baumannii clinical isolates against aminoglycosides |
topic_facet |
Acinetobacter baumannii Aminoglycoside-modifying enzymes ArmA Aminoglycoside resistance Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract INTRODUCTION: This study aimed to determine the role of genes encoding aminoglycoside-modifying enzymes (AMEs) and 16S rRNA methylase (ArmA) in Acinetobacter baumannii clinical isolates. METHODS: We collected 100 clinical isolates of A. baumannii and identified and confirmed them using microbiological tests and assessment of the OXA-51 gene. Antibiotic susceptibility testing was carried out using disk agar diffusion and micro-broth dilution methods. The presence of AME genes and ArmA was detected by PCR and multiplex PCR. RESULTS: The most and least effective antibiotics in this study were netilmicin and ciprofloxacin with 68% and 100% resistance rates, respectively. According to the minimum inhibitory concentration test, 94% of the isolates were resistant to gentamicin, tobramycin, and streptomycin, while the highest susceptibility (20%) was observed against netilmicin. The proportion of strains harboring the aminoglycoside resistance genes was as follows: APH(3′)-VIa (aphA6) (77%), ANT(2”)-Ia (aadB) (73%), ANT(3”)-Ia (aadA1) (33%), AAC(6′)-Ib (aacA4) (33%), ArmA (22%), and AAC(3)-IIa (aacC2) (19%). Among the 22 gene profiles detected in this study, the most prevalent profiles included APH(3′)-VIa + ANT(2”)-Ia (39 isolates, 100% of which were kanamycin-resistant), and AAC(3)-IIa + AAC(6′)-Ib + ANT(3”)-Ia + APH(3′)-VIa + ANT(2”)-Ia (14 isolates, all of which were resistant to gentamicin, kanamycin, and streptomycin). CONCLUSIONS: High minimum inhibitory concentration of aminoglycosides in isolates with the simultaneous presence of AME- and ArmA-encoding genes indicated the importance of these genes in resistance to aminoglycosides. However, control of their spread could be effective in the treatment of infections caused by A. baumannii. |
format |
Article in Journal/Newspaper |
author |
Maryam Asadi Jouybari Mohammad Ahanjan Bahman Mirzaei Hamid Reza Goli |
author_facet |
Maryam Asadi Jouybari Mohammad Ahanjan Bahman Mirzaei Hamid Reza Goli |
author_sort |
Maryam Asadi Jouybari |
title |
Role of aminoglycoside-modifying enzymes and 16S rRNA methylase (ArmA) in resistance of Acinetobacter baumannii clinical isolates against aminoglycosides |
title_short |
Role of aminoglycoside-modifying enzymes and 16S rRNA methylase (ArmA) in resistance of Acinetobacter baumannii clinical isolates against aminoglycosides |
title_full |
Role of aminoglycoside-modifying enzymes and 16S rRNA methylase (ArmA) in resistance of Acinetobacter baumannii clinical isolates against aminoglycosides |
title_fullStr |
Role of aminoglycoside-modifying enzymes and 16S rRNA methylase (ArmA) in resistance of Acinetobacter baumannii clinical isolates against aminoglycosides |
title_full_unstemmed |
Role of aminoglycoside-modifying enzymes and 16S rRNA methylase (ArmA) in resistance of Acinetobacter baumannii clinical isolates against aminoglycosides |
title_sort |
role of aminoglycoside-modifying enzymes and 16s rrna methylase (arma) in resistance of acinetobacter baumannii clinical isolates against aminoglycosides |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2021 |
url |
https://doi.org/10.1590/0037-8682-0599-2020 https://doaj.org/article/90c8420ca3de4c46b6b0e2711e0ca59d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 54 (2021) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822021000100305&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0599-2020 https://doaj.org/article/90c8420ca3de4c46b6b0e2711e0ca59d |
op_doi |
https://doi.org/10.1590/0037-8682-0599-2020 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
container_volume |
54 |
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