Genotypic identification of Cryptosporidium spp. isolated from hiv-infected patients and immunocompetent children of São Paulo, Brazil Identificação genotípica de Cryptosporidium spp. isolados a partir de pacientes com HIV e crianças imunocompetentes de São Paulo, Brasil
Cryptosporidium isolates identified in fourteen stool samples, collected from five HIV-infected patients and nine immunocompetent children, living in the Sate of São Paulo, Brazil, were submitted to a molecular analysis using a nested PCR followed of restriction fragment length polymorphism (RFLP),...
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ftdoajarticles:oai:doaj.org/article:90ae93a57f0947dc918c56109a03c263 2024-09-09T19:28:11+00:00 Genotypic identification of Cryptosporidium spp. isolated from hiv-infected patients and immunocompetent children of São Paulo, Brazil Identificação genotípica de Cryptosporidium spp. isolados a partir de pacientes com HIV e crianças imunocompetentes de São Paulo, Brasil Ana Julia Urias dos Santos Araújo Herminia Yohko Kanamura Marcos Eduardo de Almeida Aparecida Helena de Souza Gomes Thais Helena Lemos Pinto Alexandre Januário Da Silva 2008-06-01T00:00:00Z https://doaj.org/article/90ae93a57f0947dc918c56109a03c263 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652008000300002 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 0036-4665 1678-9946 https://doaj.org/article/90ae93a57f0947dc918c56109a03c263 Revista do Instituto de Medicina Tropical de São Paulo, Vol 50, Iss 3, Pp 139-143 (2008) Cryptosporidiosis PCR Genotyping Cryptosporidium parvum Cryptosporidium hominis Cryptosporidium meleagridis Brazil Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2008 ftdoajarticles 2024-08-05T17:49:30Z Cryptosporidium isolates identified in fourteen stool samples, collected from five HIV-infected patients and nine immunocompetent children, living in the Sate of São Paulo, Brazil, were submitted to a molecular analysis using a nested PCR followed of restriction fragment length polymorphism (RFLP), for genetic characterization. The analysis was based on digestion with RsaI restriction enzyme of a DNA fragment amplified from the Cryptosporidium oocyst wall protein (COWP) gene. Based on this analysis, four samples were identified as Cryptosporidium parvum, eight as Cryptosporidium hominis and two presented a profile that correspondedto Cryptosporidium meleagridis when compared to the standards used in the analysis. The use of molecular methods can be helpful to identify source of infections and risk factors related to Cryptosporidium infection in our communities. Isolados de Cryptosporidium identificados em quatorze amostras de fezes, coletadas de cinco pacientes com infecção por HIV e de nove crianças imunocompetentes, residentes no estado de São Paulo, Brasil, foram submetidos a análise molecular por Nested-PCR, seguido da caracterização genética por polimorfismo do tamanho do fragmento de restrição (RFLP). A análise foi baseada na digestão, com a enzima de restrição RsaI, de um fragmento de DNA amplificado do gene que codifica a proteína de parede do oocisto de Cryptosporidium (COWP). Baseado nesta análise, quando comparadas aos padrões utilizados, quatro amostras foram identificadas como Cryptosporidium parvum, oito como Cryptosporidium hominis e duas apresentaram um perfil correspondente ao de Cryptosporidium meleagridis. O uso de métodos moleculares pode ser útil para identificar a fonte das infecções e os fatores de risco relacionados à infecção por Cryptosporidium em nossas comunidades. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Cryptosporidiosis PCR Genotyping Cryptosporidium parvum Cryptosporidium hominis Cryptosporidium meleagridis Brazil Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Cryptosporidiosis PCR Genotyping Cryptosporidium parvum Cryptosporidium hominis Cryptosporidium meleagridis Brazil Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Ana Julia Urias dos Santos Araújo Herminia Yohko Kanamura Marcos Eduardo de Almeida Aparecida Helena de Souza Gomes Thais Helena Lemos Pinto Alexandre Januário Da Silva Genotypic identification of Cryptosporidium spp. isolated from hiv-infected patients and immunocompetent children of São Paulo, Brazil Identificação genotípica de Cryptosporidium spp. isolados a partir de pacientes com HIV e crianças imunocompetentes de São Paulo, Brasil |
topic_facet |
Cryptosporidiosis PCR Genotyping Cryptosporidium parvum Cryptosporidium hominis Cryptosporidium meleagridis Brazil Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Cryptosporidium isolates identified in fourteen stool samples, collected from five HIV-infected patients and nine immunocompetent children, living in the Sate of São Paulo, Brazil, were submitted to a molecular analysis using a nested PCR followed of restriction fragment length polymorphism (RFLP), for genetic characterization. The analysis was based on digestion with RsaI restriction enzyme of a DNA fragment amplified from the Cryptosporidium oocyst wall protein (COWP) gene. Based on this analysis, four samples were identified as Cryptosporidium parvum, eight as Cryptosporidium hominis and two presented a profile that correspondedto Cryptosporidium meleagridis when compared to the standards used in the analysis. The use of molecular methods can be helpful to identify source of infections and risk factors related to Cryptosporidium infection in our communities. Isolados de Cryptosporidium identificados em quatorze amostras de fezes, coletadas de cinco pacientes com infecção por HIV e de nove crianças imunocompetentes, residentes no estado de São Paulo, Brasil, foram submetidos a análise molecular por Nested-PCR, seguido da caracterização genética por polimorfismo do tamanho do fragmento de restrição (RFLP). A análise foi baseada na digestão, com a enzima de restrição RsaI, de um fragmento de DNA amplificado do gene que codifica a proteína de parede do oocisto de Cryptosporidium (COWP). Baseado nesta análise, quando comparadas aos padrões utilizados, quatro amostras foram identificadas como Cryptosporidium parvum, oito como Cryptosporidium hominis e duas apresentaram um perfil correspondente ao de Cryptosporidium meleagridis. O uso de métodos moleculares pode ser útil para identificar a fonte das infecções e os fatores de risco relacionados à infecção por Cryptosporidium em nossas comunidades. |
format |
Article in Journal/Newspaper |
author |
Ana Julia Urias dos Santos Araújo Herminia Yohko Kanamura Marcos Eduardo de Almeida Aparecida Helena de Souza Gomes Thais Helena Lemos Pinto Alexandre Januário Da Silva |
author_facet |
Ana Julia Urias dos Santos Araújo Herminia Yohko Kanamura Marcos Eduardo de Almeida Aparecida Helena de Souza Gomes Thais Helena Lemos Pinto Alexandre Januário Da Silva |
author_sort |
Ana Julia Urias dos Santos Araújo |
title |
Genotypic identification of Cryptosporidium spp. isolated from hiv-infected patients and immunocompetent children of São Paulo, Brazil Identificação genotípica de Cryptosporidium spp. isolados a partir de pacientes com HIV e crianças imunocompetentes de São Paulo, Brasil |
title_short |
Genotypic identification of Cryptosporidium spp. isolated from hiv-infected patients and immunocompetent children of São Paulo, Brazil Identificação genotípica de Cryptosporidium spp. isolados a partir de pacientes com HIV e crianças imunocompetentes de São Paulo, Brasil |
title_full |
Genotypic identification of Cryptosporidium spp. isolated from hiv-infected patients and immunocompetent children of São Paulo, Brazil Identificação genotípica de Cryptosporidium spp. isolados a partir de pacientes com HIV e crianças imunocompetentes de São Paulo, Brasil |
title_fullStr |
Genotypic identification of Cryptosporidium spp. isolated from hiv-infected patients and immunocompetent children of São Paulo, Brazil Identificação genotípica de Cryptosporidium spp. isolados a partir de pacientes com HIV e crianças imunocompetentes de São Paulo, Brasil |
title_full_unstemmed |
Genotypic identification of Cryptosporidium spp. isolated from hiv-infected patients and immunocompetent children of São Paulo, Brazil Identificação genotípica de Cryptosporidium spp. isolados a partir de pacientes com HIV e crianças imunocompetentes de São Paulo, Brasil |
title_sort |
genotypic identification of cryptosporidium spp. isolated from hiv-infected patients and immunocompetent children of são paulo, brazil identificação genotípica de cryptosporidium spp. isolados a partir de pacientes com hiv e crianças imunocompetentes de são paulo, brasil |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2008 |
url |
https://doaj.org/article/90ae93a57f0947dc918c56109a03c263 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 50, Iss 3, Pp 139-143 (2008) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652008000300002 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 0036-4665 1678-9946 https://doaj.org/article/90ae93a57f0947dc918c56109a03c263 |
_version_ |
1809897457575788544 |