Persistence of antibodies in blood and body fluids in decaying fox carcasses, as exemplified by antibodies against Microsporum canis
Abstract To assist in evaluating serological test results from dead animals, 10 silver foxes ( Vulpes vulpes ) and 10 blue foxes ( Alopex lagopus ), 6 of each species previously vaccinated against and all challenged with Microsporum canis , were blood sampled and euthanased. Fox carcasses were store...
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Online Access: | https://doi.org/10.1186/1751-0147-48-10 https://doaj.org/article/90987e2bb586422ab1c97694c0d41d92 |
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ftdoajarticles:oai:doaj.org/article:90987e2bb586422ab1c97694c0d41d92 2023-05-15T13:19:51+02:00 Persistence of antibodies in blood and body fluids in decaying fox carcasses, as exemplified by antibodies against Microsporum canis Solbakk Inge-Tom Bratberg Anna-Marie Handeland Kjell Tryland Morten Oksanen Antti 2006-06-01T00:00:00Z https://doi.org/10.1186/1751-0147-48-10 https://doaj.org/article/90987e2bb586422ab1c97694c0d41d92 EN eng BMC http://www.actavetscand.com/content/48/1/10 https://doaj.org/toc/1751-0147 doi:10.1186/1751-0147-48-10 1751-0147 https://doaj.org/article/90987e2bb586422ab1c97694c0d41d92 Acta Veterinaria Scandinavica, Vol 48, Iss 1, p 10 (2006) Veterinary medicine SF600-1100 article 2006 ftdoajarticles https://doi.org/10.1186/1751-0147-48-10 2022-12-31T08:50:37Z Abstract To assist in evaluating serological test results from dead animals, 10 silver foxes ( Vulpes vulpes ) and 10 blue foxes ( Alopex lagopus ), 6 of each species previously vaccinated against and all challenged with Microsporum canis , were blood sampled and euthanased. Fox carcasses were stored at +10°C, and autopsy was performed on Days 0, 2, 4, 7, and 11 post mortem during which samples from blood and/or body fluid from the thoracic cavity were collected. Antibodies against M. canis were measured in an enzyme-linked immunosorbent assay (ELISA) as absorbance values (optical density; OD). To assess the degradation of antibodies, the ratio between post mortem and ante mortem absorbance was calculated. The mean absorbance from samples collected during autopsy was generally lower than from samples from live animals. In blood samples, this difference increased significantly with time ( P = 0.04), while in body fluid samples the difference decreased (not significant; P = 0.18). We suggest that a positive serological result from testing blood or body fluid of a dead animal may be regarded as valuable, although specific prevalences obtained by screening populations based on this type of material may represent an under-estimation of the true antibody prevalence. Negative serological test results based on material from carcasses may be less conclusive, taken into account the general degradation processes in decaying carcasses, also involving immunoglobulin proteins. Article in Journal/Newspaper Alopex lagopus Directory of Open Access Journals: DOAJ Articles Acta Veterinaria Scandinavica 48 1 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Veterinary medicine SF600-1100 |
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Veterinary medicine SF600-1100 Solbakk Inge-Tom Bratberg Anna-Marie Handeland Kjell Tryland Morten Oksanen Antti Persistence of antibodies in blood and body fluids in decaying fox carcasses, as exemplified by antibodies against Microsporum canis |
topic_facet |
Veterinary medicine SF600-1100 |
description |
Abstract To assist in evaluating serological test results from dead animals, 10 silver foxes ( Vulpes vulpes ) and 10 blue foxes ( Alopex lagopus ), 6 of each species previously vaccinated against and all challenged with Microsporum canis , were blood sampled and euthanased. Fox carcasses were stored at +10°C, and autopsy was performed on Days 0, 2, 4, 7, and 11 post mortem during which samples from blood and/or body fluid from the thoracic cavity were collected. Antibodies against M. canis were measured in an enzyme-linked immunosorbent assay (ELISA) as absorbance values (optical density; OD). To assess the degradation of antibodies, the ratio between post mortem and ante mortem absorbance was calculated. The mean absorbance from samples collected during autopsy was generally lower than from samples from live animals. In blood samples, this difference increased significantly with time ( P = 0.04), while in body fluid samples the difference decreased (not significant; P = 0.18). We suggest that a positive serological result from testing blood or body fluid of a dead animal may be regarded as valuable, although specific prevalences obtained by screening populations based on this type of material may represent an under-estimation of the true antibody prevalence. Negative serological test results based on material from carcasses may be less conclusive, taken into account the general degradation processes in decaying carcasses, also involving immunoglobulin proteins. |
format |
Article in Journal/Newspaper |
author |
Solbakk Inge-Tom Bratberg Anna-Marie Handeland Kjell Tryland Morten Oksanen Antti |
author_facet |
Solbakk Inge-Tom Bratberg Anna-Marie Handeland Kjell Tryland Morten Oksanen Antti |
author_sort |
Solbakk Inge-Tom |
title |
Persistence of antibodies in blood and body fluids in decaying fox carcasses, as exemplified by antibodies against Microsporum canis |
title_short |
Persistence of antibodies in blood and body fluids in decaying fox carcasses, as exemplified by antibodies against Microsporum canis |
title_full |
Persistence of antibodies in blood and body fluids in decaying fox carcasses, as exemplified by antibodies against Microsporum canis |
title_fullStr |
Persistence of antibodies in blood and body fluids in decaying fox carcasses, as exemplified by antibodies against Microsporum canis |
title_full_unstemmed |
Persistence of antibodies in blood and body fluids in decaying fox carcasses, as exemplified by antibodies against Microsporum canis |
title_sort |
persistence of antibodies in blood and body fluids in decaying fox carcasses, as exemplified by antibodies against microsporum canis |
publisher |
BMC |
publishDate |
2006 |
url |
https://doi.org/10.1186/1751-0147-48-10 https://doaj.org/article/90987e2bb586422ab1c97694c0d41d92 |
genre |
Alopex lagopus |
genre_facet |
Alopex lagopus |
op_source |
Acta Veterinaria Scandinavica, Vol 48, Iss 1, p 10 (2006) |
op_relation |
http://www.actavetscand.com/content/48/1/10 https://doaj.org/toc/1751-0147 doi:10.1186/1751-0147-48-10 1751-0147 https://doaj.org/article/90987e2bb586422ab1c97694c0d41d92 |
op_doi |
https://doi.org/10.1186/1751-0147-48-10 |
container_title |
Acta Veterinaria Scandinavica |
container_volume |
48 |
container_issue |
1 |
_version_ |
1766349928572911616 |