Species-Specific N -Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction
Noroviruses, the major cause of acute viral gastroenteritis, are known to bind to histo-blood group antigens (HBGAs), including ABH groups and Lewis-type epitopes, which decorate the surface of erythrocytes and epithelial cells of their host tissues. The biosynthesis of these antigens is controlled...
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ftdoajarticles:oai:doaj.org/article:907fa189bde34b1e893aa52a838a8258 2023-07-23T04:18:54+02:00 Species-Specific N -Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction Audrey Auger Shin-Yi Yu Shih-Yun Guu Agnès Quéméner Gabriel Euller-Nicolas Hiromune Ando Marion Desdouits Françoise S. Le Guyader Kay-Hooi Khoo Jacques Le Pendu Frederic Chirat Yann Guerardel 2023-06-01T00:00:00Z https://doi.org/10.3390/md21060342 https://doaj.org/article/907fa189bde34b1e893aa52a838a8258 EN eng MDPI AG https://www.mdpi.com/1660-3397/21/6/342 https://doaj.org/toc/1660-3397 doi:10.3390/md21060342 1660-3397 https://doaj.org/article/907fa189bde34b1e893aa52a838a8258 Marine Drugs, Vol 21, Iss 342, p 342 (2023) glycomics norovirus ligands oysters methylation Biology (General) QH301-705.5 article 2023 ftdoajarticles https://doi.org/10.3390/md21060342 2023-07-02T00:37:48Z Noroviruses, the major cause of acute viral gastroenteritis, are known to bind to histo-blood group antigens (HBGAs), including ABH groups and Lewis-type epitopes, which decorate the surface of erythrocytes and epithelial cells of their host tissues. The biosynthesis of these antigens is controlled by several glycosyltransferases, the distribution and expression of which varies between tissues and individuals. The use of HBGAs as ligands by viruses is not limited to humans, as many animal species, including oysters, which synthesize similar glycan epitopes that act as a gateway for viruses, become vectors for viral infection in humans. Here, we show that different oyster species synthesize a wide range of N -glycans that share histo-blood A-antigens but differ in the expression of other terminal antigens and in their modification by O -methyl groups. In particular, we show that the N -glycans isolated from Crassostrea gigas and Ostrea edulis exhibit exquisite methylation patterns in their terminal N -acetylgalactosamine and fucose residues in terms of position and number, adding another layer of complexity to the post-translational glycosylation modifications of glycoproteins. Furthermore, modeling of the interactions between norovirus capsid proteins and carbohydrate ligands strongly suggests that methylation has the potential to fine-tune the recognition events of oysters by virus particles. Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles Marine Drugs 21 6 342 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
glycomics norovirus ligands oysters methylation Biology (General) QH301-705.5 |
spellingShingle |
glycomics norovirus ligands oysters methylation Biology (General) QH301-705.5 Audrey Auger Shin-Yi Yu Shih-Yun Guu Agnès Quéméner Gabriel Euller-Nicolas Hiromune Ando Marion Desdouits Françoise S. Le Guyader Kay-Hooi Khoo Jacques Le Pendu Frederic Chirat Yann Guerardel Species-Specific N -Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction |
topic_facet |
glycomics norovirus ligands oysters methylation Biology (General) QH301-705.5 |
description |
Noroviruses, the major cause of acute viral gastroenteritis, are known to bind to histo-blood group antigens (HBGAs), including ABH groups and Lewis-type epitopes, which decorate the surface of erythrocytes and epithelial cells of their host tissues. The biosynthesis of these antigens is controlled by several glycosyltransferases, the distribution and expression of which varies between tissues and individuals. The use of HBGAs as ligands by viruses is not limited to humans, as many animal species, including oysters, which synthesize similar glycan epitopes that act as a gateway for viruses, become vectors for viral infection in humans. Here, we show that different oyster species synthesize a wide range of N -glycans that share histo-blood A-antigens but differ in the expression of other terminal antigens and in their modification by O -methyl groups. In particular, we show that the N -glycans isolated from Crassostrea gigas and Ostrea edulis exhibit exquisite methylation patterns in their terminal N -acetylgalactosamine and fucose residues in terms of position and number, adding another layer of complexity to the post-translational glycosylation modifications of glycoproteins. Furthermore, modeling of the interactions between norovirus capsid proteins and carbohydrate ligands strongly suggests that methylation has the potential to fine-tune the recognition events of oysters by virus particles. |
format |
Article in Journal/Newspaper |
author |
Audrey Auger Shin-Yi Yu Shih-Yun Guu Agnès Quéméner Gabriel Euller-Nicolas Hiromune Ando Marion Desdouits Françoise S. Le Guyader Kay-Hooi Khoo Jacques Le Pendu Frederic Chirat Yann Guerardel |
author_facet |
Audrey Auger Shin-Yi Yu Shih-Yun Guu Agnès Quéméner Gabriel Euller-Nicolas Hiromune Ando Marion Desdouits Françoise S. Le Guyader Kay-Hooi Khoo Jacques Le Pendu Frederic Chirat Yann Guerardel |
author_sort |
Audrey Auger |
title |
Species-Specific N -Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction |
title_short |
Species-Specific N -Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction |
title_full |
Species-Specific N -Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction |
title_fullStr |
Species-Specific N -Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction |
title_full_unstemmed |
Species-Specific N -Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction |
title_sort |
species-specific n -glycomes and methylation patterns of oysters crassostrea gigas and ostrea edulis and their possible consequences for the norovirus–hbga interaction |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/md21060342 https://doaj.org/article/907fa189bde34b1e893aa52a838a8258 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Marine Drugs, Vol 21, Iss 342, p 342 (2023) |
op_relation |
https://www.mdpi.com/1660-3397/21/6/342 https://doaj.org/toc/1660-3397 doi:10.3390/md21060342 1660-3397 https://doaj.org/article/907fa189bde34b1e893aa52a838a8258 |
op_doi |
https://doi.org/10.3390/md21060342 |
container_title |
Marine Drugs |
container_volume |
21 |
container_issue |
6 |
container_start_page |
342 |
_version_ |
1772181611415076864 |