Microbial Community and Functional Gene Changes in Arctic Tundra Soils in a Microcosm Warming Experiment

• Published in: Frontiers in Microbiology
• Main Authors: Ziming Yang, Sihang Yang, Joy D. Van Nostrand, Jizhong Zhou, Wei Fang, Qi Qi, Yurong Liu, Stan D. Wullschleger, Liyuan Liang, David E. Graham, Yunfeng Yang, Baohua Gu
• Format: Article in Journal/Newspaper
• Language: English
• Published: Frontiers Media S.A. 2017
• Subjects: soil organic carbon; climate warming; microbial community; functional genes; permafrost; Microbiology; QR1-502; Arctic; Barrow; Tundra; Alaska
• Online Access: https://doi.org/10.3389/fmicb.2017.01741; https://doaj.org/article/904d88533b0d49619193cc24a1e99a94

Microbial decomposition of soil organic carbon (SOC) in thawing Arctic permafrost is important in determining greenhouse gas feedbacks of tundra ecosystems to climate. However, the changes in microbial community structure during SOC decomposition are poorly known. Here we examine these changes using frozen soils from Barrow, Alaska, USA, in anoxic microcosm incubation at −2 and 8°C for 122 days. The functional gene array GeoChip was used to determine microbial community structure and the functional genes associated with SOC degradation, methanogenesis, and Fe(III) reduction. Results show that soil incubation after 122 days at 8°C significantly decreased functional gene abundance (P < 0.05) associated with SOC degradation, fermentation, methanogenesis, and iron cycling, particularly in organic-rich soil. These observations correspond well with decreases in labile SOC content (e.g., reducing sugar and ethanol), methane and CO2 production, and Fe(III) reduction. In contrast, the community functional structure was largely unchanged in the −2°C incubation. Soil type (i.e., organic vs. mineral) and the availability of labile SOC were among the most significant factors impacting microbial community structure. These results demonstrate the important roles of microbial community in SOC degradation and support previous findings that SOC in organic-rich Arctic tundra is highly vulnerable to microbial degradation under warming.