Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients DOT-ELISA para a detecção de anticorpos IgG e IgM para antígenos de ovos e vermes de Schistosoma mansoni, em associação com excreção de ovos por pacientes
Human schistosomiasis, caused by Schistosoma mansoni, is highly prevalent in Brazil and usually diagnosed by time consuming stool analysis. Serological tests are of limited use in this disease, mainly for epidemiological studies, showing no discrimination between previous contact with the parasite a...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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Universidade de São Paulo (USP)
1995
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Online Access: | https://doi.org/10.1590/S0036-46651995000200004 https://doaj.org/article/8ff1d6d3161c48a6beab73c6df9c16c9 |
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ftdoajarticles:oai:doaj.org/article:8ff1d6d3161c48a6beab73c6df9c16c9 2024-09-09T19:28:37+00:00 Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients DOT-ELISA para a detecção de anticorpos IgG e IgM para antígenos de ovos e vermes de Schistosoma mansoni, em associação com excreção de ovos por pacientes Pedro Luiz Silva Pinto Herminia Y. Kanamura Rita Maria Silva Cristina Renata Nardoto Rossi Heitor Franco de Andrade Jr. Vicente Amato Neto 1995-04-01T00:00:00Z https://doi.org/10.1590/S0036-46651995000200004 https://doaj.org/article/8ff1d6d3161c48a6beab73c6df9c16c9 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651995000200004 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651995000200004 0036-4665 1678-9946 https://doaj.org/article/8ff1d6d3161c48a6beab73c6df9c16c9 Revista do Instituto de Medicina Tropical de São Paulo, Vol 37, Iss 2, Pp 109-115 (1995) Schistosomiasis DOT-ELISA Egg excretion Diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1995 ftdoajarticles https://doi.org/10.1590/S0036-46651995000200004 2024-08-05T17:49:30Z Human schistosomiasis, caused by Schistosoma mansoni, is highly prevalent in Brazil and usually diagnosed by time consuming stool analysis. Serological tests are of limited use in this disease, mainly for epidemiological studies, showing no discrimination between previous contact with the parasite and active infections. In the present study, we standardized and compared a Dot-ELISA for IgM and IgG antibodies against S. mansoni antigens from eggs and worms with a routine IgG and IgM immunofluorescence assay using similar antigens, in the study of sera from 27 patients who had quantified egg stool excretion. The positivity obtained for IgG Dot-ELISA was 96.3% and 88.9% for IgM Dot-ELISA with worm antigen and 92.6% and 90.9% with egg antigen. The IFI presented similar positivities using worm antigen, 92.6% (IgG) and 96.3% (IgM),and lower results with egg antigen, 77.8% (IgG and IgM). The patients studied were divided into two groups according to their egg excretion, with greater positivity of serological tests in higher egg excreters. When comparing the quantitative egg excretion and the serological titers of the patients, we detected a correlation only with IgM Dot-ELISA, with r=0.552 (p=0.0127). These data show that Dot-ELISA can be used for the detection of specific antibodies against S. mansoni in sera from suspected patients or in epidemiological studies and, with further purification of egg antigen and larger samples, IgM Dot-ELISA could be a possible tool for rough estimates of parasite burden in epidemiological studies. A esquistosomose humana, causada pelo Schistosoma mansoni, é altamente prevalente no Brasil e é usualmente diagnosticada através de exames de fezes, que são demorados. Os testes sorológicos nesta doença limitam-se aos estudos epidemiológicos, mostrando apenas o contato prévio com o parasita e não a infecção realmente ativa. Neste trabalho, nós padronizamos e comparamos um novo ensaio de Dot-ELISA, para detecção de IgM e IgG específicas contra antígenos de ovo e vermes de S. mansoni, ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 37 2 109 115 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Schistosomiasis DOT-ELISA Egg excretion Diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Schistosomiasis DOT-ELISA Egg excretion Diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Pedro Luiz Silva Pinto Herminia Y. Kanamura Rita Maria Silva Cristina Renata Nardoto Rossi Heitor Franco de Andrade Jr. Vicente Amato Neto Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients DOT-ELISA para a detecção de anticorpos IgG e IgM para antígenos de ovos e vermes de Schistosoma mansoni, em associação com excreção de ovos por pacientes |
topic_facet |
Schistosomiasis DOT-ELISA Egg excretion Diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Human schistosomiasis, caused by Schistosoma mansoni, is highly prevalent in Brazil and usually diagnosed by time consuming stool analysis. Serological tests are of limited use in this disease, mainly for epidemiological studies, showing no discrimination between previous contact with the parasite and active infections. In the present study, we standardized and compared a Dot-ELISA for IgM and IgG antibodies against S. mansoni antigens from eggs and worms with a routine IgG and IgM immunofluorescence assay using similar antigens, in the study of sera from 27 patients who had quantified egg stool excretion. The positivity obtained for IgG Dot-ELISA was 96.3% and 88.9% for IgM Dot-ELISA with worm antigen and 92.6% and 90.9% with egg antigen. The IFI presented similar positivities using worm antigen, 92.6% (IgG) and 96.3% (IgM),and lower results with egg antigen, 77.8% (IgG and IgM). The patients studied were divided into two groups according to their egg excretion, with greater positivity of serological tests in higher egg excreters. When comparing the quantitative egg excretion and the serological titers of the patients, we detected a correlation only with IgM Dot-ELISA, with r=0.552 (p=0.0127). These data show that Dot-ELISA can be used for the detection of specific antibodies against S. mansoni in sera from suspected patients or in epidemiological studies and, with further purification of egg antigen and larger samples, IgM Dot-ELISA could be a possible tool for rough estimates of parasite burden in epidemiological studies. A esquistosomose humana, causada pelo Schistosoma mansoni, é altamente prevalente no Brasil e é usualmente diagnosticada através de exames de fezes, que são demorados. Os testes sorológicos nesta doença limitam-se aos estudos epidemiológicos, mostrando apenas o contato prévio com o parasita e não a infecção realmente ativa. Neste trabalho, nós padronizamos e comparamos um novo ensaio de Dot-ELISA, para detecção de IgM e IgG específicas contra antígenos de ovo e vermes de S. mansoni, ... |
format |
Article in Journal/Newspaper |
author |
Pedro Luiz Silva Pinto Herminia Y. Kanamura Rita Maria Silva Cristina Renata Nardoto Rossi Heitor Franco de Andrade Jr. Vicente Amato Neto |
author_facet |
Pedro Luiz Silva Pinto Herminia Y. Kanamura Rita Maria Silva Cristina Renata Nardoto Rossi Heitor Franco de Andrade Jr. Vicente Amato Neto |
author_sort |
Pedro Luiz Silva Pinto |
title |
Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients DOT-ELISA para a detecção de anticorpos IgG e IgM para antígenos de ovos e vermes de Schistosoma mansoni, em associação com excreção de ovos por pacientes |
title_short |
Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients DOT-ELISA para a detecção de anticorpos IgG e IgM para antígenos de ovos e vermes de Schistosoma mansoni, em associação com excreção de ovos por pacientes |
title_full |
Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients DOT-ELISA para a detecção de anticorpos IgG e IgM para antígenos de ovos e vermes de Schistosoma mansoni, em associação com excreção de ovos por pacientes |
title_fullStr |
Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients DOT-ELISA para a detecção de anticorpos IgG e IgM para antígenos de ovos e vermes de Schistosoma mansoni, em associação com excreção de ovos por pacientes |
title_full_unstemmed |
Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients DOT-ELISA para a detecção de anticorpos IgG e IgM para antígenos de ovos e vermes de Schistosoma mansoni, em associação com excreção de ovos por pacientes |
title_sort |
dot-elisa for the detection of igm and igg antibodies to schistosoma mansoni worm and egg antigens, associated with egg excretion by patients dot-elisa para a detecção de anticorpos igg e igm para antígenos de ovos e vermes de schistosoma mansoni, em associação com excreção de ovos por pacientes |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1995 |
url |
https://doi.org/10.1590/S0036-46651995000200004 https://doaj.org/article/8ff1d6d3161c48a6beab73c6df9c16c9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 37, Iss 2, Pp 109-115 (1995) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651995000200004 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651995000200004 0036-4665 1678-9946 https://doaj.org/article/8ff1d6d3161c48a6beab73c6df9c16c9 |
op_doi |
https://doi.org/10.1590/S0036-46651995000200004 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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37 |
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