Easy and fast method for expression and native extraction of Plasmodium vivax Duffy binding protein fragments
Abstract Background The Plasmodium vivax Duffy binding protein (PvDBP) has been the most studied ligand binding human reticulocytes to date. This molecule has a cysteine-rich domain in region II (RII) which has been used as control for evaluating the target cell binding activity of several parasite...
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ftdoajarticles:oai:doaj.org/article:8f695d4e00c44ff5bb1ab3e672a234b5 2023-05-15T15:17:56+02:00 Easy and fast method for expression and native extraction of Plasmodium vivax Duffy binding protein fragments Darwin Andrés Moreno-Pérez Luis Alfredo Baquero Maritza Bermúdez Laura Alejandra Gómez-Muñoz Yahson Varela Manuel Alfonso Patarroyo 2018-02-01T00:00:00Z https://doi.org/10.1186/s12936-018-2216-6 https://doaj.org/article/8f695d4e00c44ff5bb1ab3e672a234b5 EN eng BMC http://link.springer.com/article/10.1186/s12936-018-2216-6 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2216-6 1475-2875 https://doaj.org/article/8f695d4e00c44ff5bb1ab3e672a234b5 Malaria Journal, Vol 17, Iss 1, Pp 1-9 (2018) Plasmodium vivax Reticulocyte Duffy binding protein Soluble extraction Protein–cell interaction Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2018 ftdoajarticles https://doi.org/10.1186/s12936-018-2216-6 2022-12-31T05:17:59Z Abstract Background The Plasmodium vivax Duffy binding protein (PvDBP) has been the most studied ligand binding human reticulocytes to date. This molecule has a cysteine-rich domain in region II (RII) which has been used as control for evaluating the target cell binding activity of several parasite molecules. However, obtaining rPvDBP-RII in a soluble form using the Escherichia coli expression system usually requires laborious and time-consuming steps for recovering the molecule’s structure and function, considering it is extracted from inclusion bodies. The present study describes an easy and fast method for expressing and obtaining several PvDBP fragments which should prove ideal for use in protein–cell interaction assays. Results Two PvDBP encoding regions (rii and riii/v) were cloned in pEXP5-CT vector and expressed in E. coli and extracted from the soluble fraction (rPvDBP-RIIS and rPvDBP-RIII/VS) using a simple freezing/thawing protocol. After the purification, dichroism analysis enabled verifying high rPvDBP-RIIS and rPvDBP-RIII/VS secondary structure α-helix content, which was lowered when molecules were extracted from inclusion bodies (rPvDBP-RIIIB and rPvDBP-RIII/VIB) using a denaturing step. Interestingly, rPvDBP-RIIS, but not rPvDBP-RIIIB, bound to human reticulocytes, while rPvDBP-RIII/VS and rPvDBP-RIII/VIB bound to such cells in a similar way to negative control (cells incubated without recombinant proteins). Conclusions This research has shown for the first time how rPvDBP-RII can be expressed and obtained in soluble form using the E. coli system and avoiding the denaturation and refolding steps commonly used. The results highlight the usefulness of the rPvDBP-RIII/VS fragment as a non-binding control for protein-cell target interaction assays. The soluble extraction protocol described is a good alternative to obtain fully functional P. vivax proteins in a fast and easy way, which will surely prove useful to laboratories working in studying this parasite’s biology. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 17 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Plasmodium vivax Reticulocyte Duffy binding protein Soluble extraction Protein–cell interaction Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Plasmodium vivax Reticulocyte Duffy binding protein Soluble extraction Protein–cell interaction Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Darwin Andrés Moreno-Pérez Luis Alfredo Baquero Maritza Bermúdez Laura Alejandra Gómez-Muñoz Yahson Varela Manuel Alfonso Patarroyo Easy and fast method for expression and native extraction of Plasmodium vivax Duffy binding protein fragments |
topic_facet |
Plasmodium vivax Reticulocyte Duffy binding protein Soluble extraction Protein–cell interaction Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The Plasmodium vivax Duffy binding protein (PvDBP) has been the most studied ligand binding human reticulocytes to date. This molecule has a cysteine-rich domain in region II (RII) which has been used as control for evaluating the target cell binding activity of several parasite molecules. However, obtaining rPvDBP-RII in a soluble form using the Escherichia coli expression system usually requires laborious and time-consuming steps for recovering the molecule’s structure and function, considering it is extracted from inclusion bodies. The present study describes an easy and fast method for expressing and obtaining several PvDBP fragments which should prove ideal for use in protein–cell interaction assays. Results Two PvDBP encoding regions (rii and riii/v) were cloned in pEXP5-CT vector and expressed in E. coli and extracted from the soluble fraction (rPvDBP-RIIS and rPvDBP-RIII/VS) using a simple freezing/thawing protocol. After the purification, dichroism analysis enabled verifying high rPvDBP-RIIS and rPvDBP-RIII/VS secondary structure α-helix content, which was lowered when molecules were extracted from inclusion bodies (rPvDBP-RIIIB and rPvDBP-RIII/VIB) using a denaturing step. Interestingly, rPvDBP-RIIS, but not rPvDBP-RIIIB, bound to human reticulocytes, while rPvDBP-RIII/VS and rPvDBP-RIII/VIB bound to such cells in a similar way to negative control (cells incubated without recombinant proteins). Conclusions This research has shown for the first time how rPvDBP-RII can be expressed and obtained in soluble form using the E. coli system and avoiding the denaturation and refolding steps commonly used. The results highlight the usefulness of the rPvDBP-RIII/VS fragment as a non-binding control for protein-cell target interaction assays. The soluble extraction protocol described is a good alternative to obtain fully functional P. vivax proteins in a fast and easy way, which will surely prove useful to laboratories working in studying this parasite’s biology. |
format |
Article in Journal/Newspaper |
author |
Darwin Andrés Moreno-Pérez Luis Alfredo Baquero Maritza Bermúdez Laura Alejandra Gómez-Muñoz Yahson Varela Manuel Alfonso Patarroyo |
author_facet |
Darwin Andrés Moreno-Pérez Luis Alfredo Baquero Maritza Bermúdez Laura Alejandra Gómez-Muñoz Yahson Varela Manuel Alfonso Patarroyo |
author_sort |
Darwin Andrés Moreno-Pérez |
title |
Easy and fast method for expression and native extraction of Plasmodium vivax Duffy binding protein fragments |
title_short |
Easy and fast method for expression and native extraction of Plasmodium vivax Duffy binding protein fragments |
title_full |
Easy and fast method for expression and native extraction of Plasmodium vivax Duffy binding protein fragments |
title_fullStr |
Easy and fast method for expression and native extraction of Plasmodium vivax Duffy binding protein fragments |
title_full_unstemmed |
Easy and fast method for expression and native extraction of Plasmodium vivax Duffy binding protein fragments |
title_sort |
easy and fast method for expression and native extraction of plasmodium vivax duffy binding protein fragments |
publisher |
BMC |
publishDate |
2018 |
url |
https://doi.org/10.1186/s12936-018-2216-6 https://doaj.org/article/8f695d4e00c44ff5bb1ab3e672a234b5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 17, Iss 1, Pp 1-9 (2018) |
op_relation |
http://link.springer.com/article/10.1186/s12936-018-2216-6 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2216-6 1475-2875 https://doaj.org/article/8f695d4e00c44ff5bb1ab3e672a234b5 |
op_doi |
https://doi.org/10.1186/s12936-018-2216-6 |
container_title |
Malaria Journal |
container_volume |
17 |
container_issue |
1 |
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1766348182215720960 |