Anti-Inflammatory Effects of Metabolites from Antarctic Fungal Strain Pleosporales sp. SF-7343 in HaCaT Human Keratinocytes
Chemical investigation of the Antarctic fungi Pleosporales sp. SF-7343 revealed four known secondary fungal metabolites: alternate C ( 1 ), altenusin ( 2 ), alternariol ( 3 ), and altenuene ( 4 ). The compound structures were identified primarily by NMR and MS analyses. Atopic dermatitis, an inflamm...
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ftdoajarticles:oai:doaj.org/article:8f66ce3af22f46d1842c98fef1041354 2023-05-15T13:51:50+02:00 Anti-Inflammatory Effects of Metabolites from Antarctic Fungal Strain Pleosporales sp. SF-7343 in HaCaT Human Keratinocytes Linsha Dong Hye Jin Kim Thao Quyen Cao Zhiming Liu Hwan Lee Wonmin Ko Youn-Chul Kim Jae Hak Sohn Tai Kyoung Kim Joung Han Yim Dong-Sung Lee Hyuncheol Oh 2021-09-01T00:00:00Z https://doi.org/10.3390/ijms22189674 https://doaj.org/article/8f66ce3af22f46d1842c98fef1041354 EN eng MDPI AG https://www.mdpi.com/1422-0067/22/18/9674 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms22189674 1422-0067 1661-6596 https://doaj.org/article/8f66ce3af22f46d1842c98fef1041354 International Journal of Molecular Sciences, Vol 22, Iss 9674, p 9674 (2021) Antarctic fungi inflammation ICAM-1 NF-κB HO-1 HaCaT Biology (General) QH301-705.5 Chemistry QD1-999 article 2021 ftdoajarticles https://doi.org/10.3390/ijms22189674 2022-12-31T13:07:52Z Chemical investigation of the Antarctic fungi Pleosporales sp. SF-7343 revealed four known secondary fungal metabolites: alternate C ( 1 ), altenusin ( 2 ), alternariol ( 3 ), and altenuene ( 4 ). The compound structures were identified primarily by NMR and MS analyses. Atopic dermatitis, an inflammatory disease, is driven by the abnormal activation of T helper (Th) 2 cells and barrier dysfunction. We attempted to identify the anti-inflammatory components of SF-7343. Initial screening showed that compounds 1 and 3 inhibited the secretion of interleukin-8 and -6 in tumor necrosis factor-α/interferon-γ-treated HaCaT cells, and these compounds also showed inhibitory effects on CCL5 and CCL22. Compounds 1 and 3 also downregulated the protein expression levels of intercellular adhesion molecule-1 and upregulated the expression of filaggrin and involcurin. The mechanism study results showed that compounds 1 and 3 inhibited nuclear translocation of nuclear factor-kappa B p65 and the phosphorylation of STAT1 and STAT3. Compound 1 , but not compound 3 , significantly promoted the expression of heme oxygenase (HO)-1. The effects of compound 1 were partly reversed by co-treatment with a HO-1 inhibitor, tin protoporphyrin IX. Taken together, this study demonstrates the potential value of Antarctic fungal strain SF-7343 isolates as a bioresource for bioactive compounds to prevent skin inflammation. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic International Journal of Molecular Sciences 22 18 9674 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antarctic fungi inflammation ICAM-1 NF-κB HO-1 HaCaT Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
Antarctic fungi inflammation ICAM-1 NF-κB HO-1 HaCaT Biology (General) QH301-705.5 Chemistry QD1-999 Linsha Dong Hye Jin Kim Thao Quyen Cao Zhiming Liu Hwan Lee Wonmin Ko Youn-Chul Kim Jae Hak Sohn Tai Kyoung Kim Joung Han Yim Dong-Sung Lee Hyuncheol Oh Anti-Inflammatory Effects of Metabolites from Antarctic Fungal Strain Pleosporales sp. SF-7343 in HaCaT Human Keratinocytes |
topic_facet |
Antarctic fungi inflammation ICAM-1 NF-κB HO-1 HaCaT Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
Chemical investigation of the Antarctic fungi Pleosporales sp. SF-7343 revealed four known secondary fungal metabolites: alternate C ( 1 ), altenusin ( 2 ), alternariol ( 3 ), and altenuene ( 4 ). The compound structures were identified primarily by NMR and MS analyses. Atopic dermatitis, an inflammatory disease, is driven by the abnormal activation of T helper (Th) 2 cells and barrier dysfunction. We attempted to identify the anti-inflammatory components of SF-7343. Initial screening showed that compounds 1 and 3 inhibited the secretion of interleukin-8 and -6 in tumor necrosis factor-α/interferon-γ-treated HaCaT cells, and these compounds also showed inhibitory effects on CCL5 and CCL22. Compounds 1 and 3 also downregulated the protein expression levels of intercellular adhesion molecule-1 and upregulated the expression of filaggrin and involcurin. The mechanism study results showed that compounds 1 and 3 inhibited nuclear translocation of nuclear factor-kappa B p65 and the phosphorylation of STAT1 and STAT3. Compound 1 , but not compound 3 , significantly promoted the expression of heme oxygenase (HO)-1. The effects of compound 1 were partly reversed by co-treatment with a HO-1 inhibitor, tin protoporphyrin IX. Taken together, this study demonstrates the potential value of Antarctic fungal strain SF-7343 isolates as a bioresource for bioactive compounds to prevent skin inflammation. |
format |
Article in Journal/Newspaper |
author |
Linsha Dong Hye Jin Kim Thao Quyen Cao Zhiming Liu Hwan Lee Wonmin Ko Youn-Chul Kim Jae Hak Sohn Tai Kyoung Kim Joung Han Yim Dong-Sung Lee Hyuncheol Oh |
author_facet |
Linsha Dong Hye Jin Kim Thao Quyen Cao Zhiming Liu Hwan Lee Wonmin Ko Youn-Chul Kim Jae Hak Sohn Tai Kyoung Kim Joung Han Yim Dong-Sung Lee Hyuncheol Oh |
author_sort |
Linsha Dong |
title |
Anti-Inflammatory Effects of Metabolites from Antarctic Fungal Strain Pleosporales sp. SF-7343 in HaCaT Human Keratinocytes |
title_short |
Anti-Inflammatory Effects of Metabolites from Antarctic Fungal Strain Pleosporales sp. SF-7343 in HaCaT Human Keratinocytes |
title_full |
Anti-Inflammatory Effects of Metabolites from Antarctic Fungal Strain Pleosporales sp. SF-7343 in HaCaT Human Keratinocytes |
title_fullStr |
Anti-Inflammatory Effects of Metabolites from Antarctic Fungal Strain Pleosporales sp. SF-7343 in HaCaT Human Keratinocytes |
title_full_unstemmed |
Anti-Inflammatory Effects of Metabolites from Antarctic Fungal Strain Pleosporales sp. SF-7343 in HaCaT Human Keratinocytes |
title_sort |
anti-inflammatory effects of metabolites from antarctic fungal strain pleosporales sp. sf-7343 in hacat human keratinocytes |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/ijms22189674 https://doaj.org/article/8f66ce3af22f46d1842c98fef1041354 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
International Journal of Molecular Sciences, Vol 22, Iss 9674, p 9674 (2021) |
op_relation |
https://www.mdpi.com/1422-0067/22/18/9674 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms22189674 1422-0067 1661-6596 https://doaj.org/article/8f66ce3af22f46d1842c98fef1041354 |
op_doi |
https://doi.org/10.3390/ijms22189674 |
container_title |
International Journal of Molecular Sciences |
container_volume |
22 |
container_issue |
18 |
container_start_page |
9674 |
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1766255880502771712 |