Propolis anti-inflammatory effects on MAGE-1 and retinoic acid-treated dendritic cells and on Th1 and T regulatory cells
Abstract Background: Propolis exhibits huge potential in the pharmaceutical industry. In the present study, its effects were investigated on dendritic cells (DCs) stimulated with a tumor antigen (MAGE-1) and retinoic acid (RA) and on T lymphocytes to observe a possible differential activation of T l...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:8f38ce5cd8fc4695b740345fd4dd97c1 2023-05-15T15:07:44+02:00 Propolis anti-inflammatory effects on MAGE-1 and retinoic acid-treated dendritic cells and on Th1 and T regulatory cells Karina Basso Santiago Bruno José Conti Eliza de Oliveira Cardoso Fernanda Lopes Conte Karen Ingrid Tasca Graziela Gorete Romagnoli Marjorie de Assis Golim Maria Tereza Cruz José Maurício Sforcin 2023-01-01T00:00:00Z https://doi.org/10.1590/1678-9199-jvatitd-2022-0044 https://doaj.org/article/8f38ce5cd8fc4695b740345fd4dd97c1 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992023000100302&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2022-0044 https://doaj.org/article/8f38ce5cd8fc4695b740345fd4dd97c1 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 29 (2023) Dendritic cells T CD4+ cells MAGE-1 Retinoic acid Propolis Immunomodulation Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2023 ftdoajarticles https://doi.org/10.1590/1678-9199-jvatitd-2022-0044 2023-01-22T01:39:17Z Abstract Background: Propolis exhibits huge potential in the pharmaceutical industry. In the present study, its effects were investigated on dendritic cells (DCs) stimulated with a tumor antigen (MAGE-1) and retinoic acid (RA) and on T lymphocytes to observe a possible differential activation of T lymphocytes, driving preferentially to Th1 or Treg cells. Methods: Cell viability, lymphocyte proliferation, gene expression (T-bet and FoxP3), and cytokine production by DCs (TNF-α, IL-10, IL-6 and IL-1β) and lymphocytes (IFN-γ and TGF-β) were analyzed. Results: MAGE-1 and RA alone or in combination with propolis inhibited TNF-α production and induced a higher lymphoproliferation compared to control, while MAGE-1 + propolis induced IL-6 production. Propolis in combination with RA induced FoxP3 expression. MAGE-1 induced IFN-γ production while propolis inhibited it, returning to basal levels. RA inhibited TGF-β production, what was counteracted by propolis. Conclusion: Propolis affected immunological parameters inhibiting pro-inflammatory cytokines and favoring the regulatory profile, opening perspectives for the control of inflammatory conditions. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 29 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Dendritic cells T CD4+ cells MAGE-1 Retinoic acid Propolis Immunomodulation Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Dendritic cells T CD4+ cells MAGE-1 Retinoic acid Propolis Immunomodulation Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Karina Basso Santiago Bruno José Conti Eliza de Oliveira Cardoso Fernanda Lopes Conte Karen Ingrid Tasca Graziela Gorete Romagnoli Marjorie de Assis Golim Maria Tereza Cruz José Maurício Sforcin Propolis anti-inflammatory effects on MAGE-1 and retinoic acid-treated dendritic cells and on Th1 and T regulatory cells |
topic_facet |
Dendritic cells T CD4+ cells MAGE-1 Retinoic acid Propolis Immunomodulation Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background: Propolis exhibits huge potential in the pharmaceutical industry. In the present study, its effects were investigated on dendritic cells (DCs) stimulated with a tumor antigen (MAGE-1) and retinoic acid (RA) and on T lymphocytes to observe a possible differential activation of T lymphocytes, driving preferentially to Th1 or Treg cells. Methods: Cell viability, lymphocyte proliferation, gene expression (T-bet and FoxP3), and cytokine production by DCs (TNF-α, IL-10, IL-6 and IL-1β) and lymphocytes (IFN-γ and TGF-β) were analyzed. Results: MAGE-1 and RA alone or in combination with propolis inhibited TNF-α production and induced a higher lymphoproliferation compared to control, while MAGE-1 + propolis induced IL-6 production. Propolis in combination with RA induced FoxP3 expression. MAGE-1 induced IFN-γ production while propolis inhibited it, returning to basal levels. RA inhibited TGF-β production, what was counteracted by propolis. Conclusion: Propolis affected immunological parameters inhibiting pro-inflammatory cytokines and favoring the regulatory profile, opening perspectives for the control of inflammatory conditions. |
format |
Article in Journal/Newspaper |
author |
Karina Basso Santiago Bruno José Conti Eliza de Oliveira Cardoso Fernanda Lopes Conte Karen Ingrid Tasca Graziela Gorete Romagnoli Marjorie de Assis Golim Maria Tereza Cruz José Maurício Sforcin |
author_facet |
Karina Basso Santiago Bruno José Conti Eliza de Oliveira Cardoso Fernanda Lopes Conte Karen Ingrid Tasca Graziela Gorete Romagnoli Marjorie de Assis Golim Maria Tereza Cruz José Maurício Sforcin |
author_sort |
Karina Basso Santiago |
title |
Propolis anti-inflammatory effects on MAGE-1 and retinoic acid-treated dendritic cells and on Th1 and T regulatory cells |
title_short |
Propolis anti-inflammatory effects on MAGE-1 and retinoic acid-treated dendritic cells and on Th1 and T regulatory cells |
title_full |
Propolis anti-inflammatory effects on MAGE-1 and retinoic acid-treated dendritic cells and on Th1 and T regulatory cells |
title_fullStr |
Propolis anti-inflammatory effects on MAGE-1 and retinoic acid-treated dendritic cells and on Th1 and T regulatory cells |
title_full_unstemmed |
Propolis anti-inflammatory effects on MAGE-1 and retinoic acid-treated dendritic cells and on Th1 and T regulatory cells |
title_sort |
propolis anti-inflammatory effects on mage-1 and retinoic acid-treated dendritic cells and on th1 and t regulatory cells |
publisher |
SciELO |
publishDate |
2023 |
url |
https://doi.org/10.1590/1678-9199-jvatitd-2022-0044 https://doaj.org/article/8f38ce5cd8fc4695b740345fd4dd97c1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 29 (2023) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992023000100302&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2022-0044 https://doaj.org/article/8f38ce5cd8fc4695b740345fd4dd97c1 |
op_doi |
https://doi.org/10.1590/1678-9199-jvatitd-2022-0044 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
29 |
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1766339171098558464 |