Proteomic characterization of the thermostable toxins from Naja naja venom
Naja naja snake venom presents abundant thermostable peptides. Many of them possess useful pharmacological activity that may be employed for drug development. For the proteomic characterization of such toxins, in the present study, Naja naja venom solution was heated up to 100°C for 10, 30, 60, 120,...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:8f109fd65ef44fa28a2086a295a529f9 2023-05-15T15:05:39+02:00 Proteomic characterization of the thermostable toxins from Naja naja venom DV Binh TT Thanh PV Chi 2010-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992010000400014 https://doaj.org/article/8f109fd65ef44fa28a2086a295a529f9 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400014 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992010000400014 1678-9199 https://doaj.org/article/8f109fd65ef44fa28a2086a295a529f9 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 16, Iss 4, Pp 631-638 (2010) Naja naja snake venom proteome thermostable peptides mass spectrometry analgesic activity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2010 ftdoajarticles https://doi.org/10.1590/S1678-91992010000400014 2022-12-31T12:43:43Z Naja naja snake venom presents abundant thermostable peptides. Many of them possess useful pharmacological activity that may be employed for drug development. For the proteomic characterization of such toxins, in the present study, Naja naja venom solution was heated up to 100°C for 10, 30, 60, 120, 180 and 300 minutes and protein fractions of non-heated and heated venom were analyzed by two-dimensional nano-liquid chromatography coupled online with tandem mass spectrometry. After heating for 300 minutes, a total of 32 peptides were still detected in the supernatant. The identified peptides belong to the following groups: cardiotoxins, neurotoxins and cytotoxins. It was found that thermostable peptides are able to preserve their analgesic activity after a long heating time in formalin test. Mice injected with 15 μg/g of 60-minute heated venom or with 25 μg/g of 300-minute heated venom revealed even a better analgesic activity than those treated with lidocaine. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 16 4 631 638 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Naja naja snake venom proteome thermostable peptides mass spectrometry analgesic activity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Naja naja snake venom proteome thermostable peptides mass spectrometry analgesic activity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 DV Binh TT Thanh PV Chi Proteomic characterization of the thermostable toxins from Naja naja venom |
topic_facet |
Naja naja snake venom proteome thermostable peptides mass spectrometry analgesic activity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Naja naja snake venom presents abundant thermostable peptides. Many of them possess useful pharmacological activity that may be employed for drug development. For the proteomic characterization of such toxins, in the present study, Naja naja venom solution was heated up to 100°C for 10, 30, 60, 120, 180 and 300 minutes and protein fractions of non-heated and heated venom were analyzed by two-dimensional nano-liquid chromatography coupled online with tandem mass spectrometry. After heating for 300 minutes, a total of 32 peptides were still detected in the supernatant. The identified peptides belong to the following groups: cardiotoxins, neurotoxins and cytotoxins. It was found that thermostable peptides are able to preserve their analgesic activity after a long heating time in formalin test. Mice injected with 15 μg/g of 60-minute heated venom or with 25 μg/g of 300-minute heated venom revealed even a better analgesic activity than those treated with lidocaine. |
format |
Article in Journal/Newspaper |
author |
DV Binh TT Thanh PV Chi |
author_facet |
DV Binh TT Thanh PV Chi |
author_sort |
DV Binh |
title |
Proteomic characterization of the thermostable toxins from Naja naja venom |
title_short |
Proteomic characterization of the thermostable toxins from Naja naja venom |
title_full |
Proteomic characterization of the thermostable toxins from Naja naja venom |
title_fullStr |
Proteomic characterization of the thermostable toxins from Naja naja venom |
title_full_unstemmed |
Proteomic characterization of the thermostable toxins from Naja naja venom |
title_sort |
proteomic characterization of the thermostable toxins from naja naja venom |
publisher |
SciELO |
publishDate |
2010 |
url |
https://doi.org/10.1590/S1678-91992010000400014 https://doaj.org/article/8f109fd65ef44fa28a2086a295a529f9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 16, Iss 4, Pp 631-638 (2010) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400014 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992010000400014 1678-9199 https://doaj.org/article/8f109fd65ef44fa28a2086a295a529f9 |
op_doi |
https://doi.org/10.1590/S1678-91992010000400014 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
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16 |
container_issue |
4 |
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631 |
op_container_end_page |
638 |
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1766337305702825984 |