Identification of Novel Glycans in the Mucus Layer of Shark and Skate Skin
The mucus layer covering the skin of fish has several roles, including protection against pathogens and mechanical damage. While the mucus layers of various bony fish species have been investigated, the composition and glycan profiles of shark skin mucus remain relatively unexplored. In this pilot s...
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ftdoajarticles:oai:doaj.org/article:8ee9ec609b2445e3861e128609c2a64c 2023-10-29T02:40:48+01:00 Identification of Novel Glycans in the Mucus Layer of Shark and Skate Skin Etty Bachar-Wikstrom Kristina A. Thomsson Carina Sihlbom Lisa Abbo Haitham Tartor Sara K. Lindén Jakob D. Wikstrom 2023-09-01T00:00:00Z https://doi.org/10.3390/ijms241814331 https://doaj.org/article/8ee9ec609b2445e3861e128609c2a64c EN eng MDPI AG https://www.mdpi.com/1422-0067/24/18/14331 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms241814331 1422-0067 1661-6596 https://doaj.org/article/8ee9ec609b2445e3861e128609c2a64c International Journal of Molecular Sciences, Vol 24, Iss 14331, p 14331 (2023) elasmobranchs sharks skin mucus layer mucin glycans Biology (General) QH301-705.5 Chemistry QD1-999 article 2023 ftdoajarticles https://doi.org/10.3390/ijms241814331 2023-10-01T00:38:04Z The mucus layer covering the skin of fish has several roles, including protection against pathogens and mechanical damage. While the mucus layers of various bony fish species have been investigated, the composition and glycan profiles of shark skin mucus remain relatively unexplored. In this pilot study, we aimed to explore the structure and composition of shark skin mucus through histological analysis and glycan profiling. Histological examination of skin samples from Atlantic spiny dogfish ( Squalus acanthias ) sharks and chain catsharks ( Scyliorhinus retifer ) revealed distinct mucin-producing cells and a mucus layer, indicating the presence of a functional mucus layer similar to bony fish mucus albeit thinner. Glycan profiling using liquid chromatography–electrospray ionization tandem mass spectrometry unveiled a diverse repertoire of mostly O -glycans in the mucus of the two sharks as well as little skate ( Leucoraja erinacea ). Elasmobranch glycans differ significantly from bony fish, especially in being more sulfated, and some bear resemblance to human glycans, such as gastric mucin O -glycans and H blood group-type glycans. This study contributes to the concept of shark skin having unique properties and provides a foundation for further research into the functional roles and potential biomedical implications of shark skin mucus glycans. Article in Journal/Newspaper spiny dogfish Squalus acanthias Directory of Open Access Journals: DOAJ Articles International Journal of Molecular Sciences 24 18 14331 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
elasmobranchs sharks skin mucus layer mucin glycans Biology (General) QH301-705.5 Chemistry QD1-999 |
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elasmobranchs sharks skin mucus layer mucin glycans Biology (General) QH301-705.5 Chemistry QD1-999 Etty Bachar-Wikstrom Kristina A. Thomsson Carina Sihlbom Lisa Abbo Haitham Tartor Sara K. Lindén Jakob D. Wikstrom Identification of Novel Glycans in the Mucus Layer of Shark and Skate Skin |
topic_facet |
elasmobranchs sharks skin mucus layer mucin glycans Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
The mucus layer covering the skin of fish has several roles, including protection against pathogens and mechanical damage. While the mucus layers of various bony fish species have been investigated, the composition and glycan profiles of shark skin mucus remain relatively unexplored. In this pilot study, we aimed to explore the structure and composition of shark skin mucus through histological analysis and glycan profiling. Histological examination of skin samples from Atlantic spiny dogfish ( Squalus acanthias ) sharks and chain catsharks ( Scyliorhinus retifer ) revealed distinct mucin-producing cells and a mucus layer, indicating the presence of a functional mucus layer similar to bony fish mucus albeit thinner. Glycan profiling using liquid chromatography–electrospray ionization tandem mass spectrometry unveiled a diverse repertoire of mostly O -glycans in the mucus of the two sharks as well as little skate ( Leucoraja erinacea ). Elasmobranch glycans differ significantly from bony fish, especially in being more sulfated, and some bear resemblance to human glycans, such as gastric mucin O -glycans and H blood group-type glycans. This study contributes to the concept of shark skin having unique properties and provides a foundation for further research into the functional roles and potential biomedical implications of shark skin mucus glycans. |
format |
Article in Journal/Newspaper |
author |
Etty Bachar-Wikstrom Kristina A. Thomsson Carina Sihlbom Lisa Abbo Haitham Tartor Sara K. Lindén Jakob D. Wikstrom |
author_facet |
Etty Bachar-Wikstrom Kristina A. Thomsson Carina Sihlbom Lisa Abbo Haitham Tartor Sara K. Lindén Jakob D. Wikstrom |
author_sort |
Etty Bachar-Wikstrom |
title |
Identification of Novel Glycans in the Mucus Layer of Shark and Skate Skin |
title_short |
Identification of Novel Glycans in the Mucus Layer of Shark and Skate Skin |
title_full |
Identification of Novel Glycans in the Mucus Layer of Shark and Skate Skin |
title_fullStr |
Identification of Novel Glycans in the Mucus Layer of Shark and Skate Skin |
title_full_unstemmed |
Identification of Novel Glycans in the Mucus Layer of Shark and Skate Skin |
title_sort |
identification of novel glycans in the mucus layer of shark and skate skin |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/ijms241814331 https://doaj.org/article/8ee9ec609b2445e3861e128609c2a64c |
genre |
spiny dogfish Squalus acanthias |
genre_facet |
spiny dogfish Squalus acanthias |
op_source |
International Journal of Molecular Sciences, Vol 24, Iss 14331, p 14331 (2023) |
op_relation |
https://www.mdpi.com/1422-0067/24/18/14331 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms241814331 1422-0067 1661-6596 https://doaj.org/article/8ee9ec609b2445e3861e128609c2a64c |
op_doi |
https://doi.org/10.3390/ijms241814331 |
container_title |
International Journal of Molecular Sciences |
container_volume |
24 |
container_issue |
18 |
container_start_page |
14331 |
_version_ |
1781069742743748608 |