Transcriptome analyses of Atlantic salmon muscle genes induced by a DNA vaccine against salmonid alphavirus, the causative agent of salmon pancreas disease (PD).
Salmonid alphavirus (SAV) is the causative agent of pancreas disease (PD) in farmed Atlantic salmon. A previous study showed that vaccination of pre-smolt salmon with a plasmid encoding the structural polypeptide of SAV gave protection against infection and development of PD accompanied by productio...
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ftdoajarticles:oai:doaj.org/article:8ee0c87ea99047118fbb10382bf0c5f0 2023-05-15T15:31:47+02:00 Transcriptome analyses of Atlantic salmon muscle genes induced by a DNA vaccine against salmonid alphavirus, the causative agent of salmon pancreas disease (PD). Mehrdad Sobhkhez Aleksei Krasnov Børre Robertsen 2018-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0204924 https://doaj.org/article/8ee0c87ea99047118fbb10382bf0c5f0 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC6166962?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0204924 https://doaj.org/article/8ee0c87ea99047118fbb10382bf0c5f0 PLoS ONE, Vol 13, Iss 10, p e0204924 (2018) Medicine R Science Q article 2018 ftdoajarticles https://doi.org/10.1371/journal.pone.0204924 2022-12-31T15:53:07Z Salmonid alphavirus (SAV) is the causative agent of pancreas disease (PD) in farmed Atlantic salmon. A previous study showed that vaccination of pre-smolt salmon with a plasmid encoding the structural polypeptide of SAV gave protection against infection and development of PD accompanied by production of antibodies against the virus. In the present work we analyzed transcript responses in the muscle to vaccination with this plasmid (here named pSAV). The purpose was to shed light on how pSAV might initiate adaptive immune responses in the fish. The work was based on microarray and reverse transcription quantitative PCR analyses of muscle at the injection site 7 days after vaccination. The results showed that pSAV and pcDNA3.3 had similar abilities to up-regulate type I IFN stimulated genes. In contrast, pSAV caused higher up-regulation of IFNγ and several IFNγ inducible genes. Compared to pcDNA3.3, pSAV also gave larger increase in transcripts of marker genes for B-cells, T-cells and antigen presenting cells (APCs), which suggest attraction and role of these cells in the adaptive immune responses elicited by pSAV. Moreover, pSAV caused a stronger up-regulation of the chemokine CXCL10 and the proinflammatory cytokines IL-1ß and TNFα, which may explain attraction of lymphocytes and APCs. The present work shows that the expression profile of genes resulting from vaccination with pSAV is different from the expression profiles obtained previously by vaccination of salmonids with DNA vaccines against infectious salmon anemia virus and infectious hematopoietic necrosis virus. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles Sav’ ENVELOPE(156.400,156.400,68.817,68.817) PLOS ONE 13 10 e0204924 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Medicine R Science Q |
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Medicine R Science Q Mehrdad Sobhkhez Aleksei Krasnov Børre Robertsen Transcriptome analyses of Atlantic salmon muscle genes induced by a DNA vaccine against salmonid alphavirus, the causative agent of salmon pancreas disease (PD). |
topic_facet |
Medicine R Science Q |
description |
Salmonid alphavirus (SAV) is the causative agent of pancreas disease (PD) in farmed Atlantic salmon. A previous study showed that vaccination of pre-smolt salmon with a plasmid encoding the structural polypeptide of SAV gave protection against infection and development of PD accompanied by production of antibodies against the virus. In the present work we analyzed transcript responses in the muscle to vaccination with this plasmid (here named pSAV). The purpose was to shed light on how pSAV might initiate adaptive immune responses in the fish. The work was based on microarray and reverse transcription quantitative PCR analyses of muscle at the injection site 7 days after vaccination. The results showed that pSAV and pcDNA3.3 had similar abilities to up-regulate type I IFN stimulated genes. In contrast, pSAV caused higher up-regulation of IFNγ and several IFNγ inducible genes. Compared to pcDNA3.3, pSAV also gave larger increase in transcripts of marker genes for B-cells, T-cells and antigen presenting cells (APCs), which suggest attraction and role of these cells in the adaptive immune responses elicited by pSAV. Moreover, pSAV caused a stronger up-regulation of the chemokine CXCL10 and the proinflammatory cytokines IL-1ß and TNFα, which may explain attraction of lymphocytes and APCs. The present work shows that the expression profile of genes resulting from vaccination with pSAV is different from the expression profiles obtained previously by vaccination of salmonids with DNA vaccines against infectious salmon anemia virus and infectious hematopoietic necrosis virus. |
format |
Article in Journal/Newspaper |
author |
Mehrdad Sobhkhez Aleksei Krasnov Børre Robertsen |
author_facet |
Mehrdad Sobhkhez Aleksei Krasnov Børre Robertsen |
author_sort |
Mehrdad Sobhkhez |
title |
Transcriptome analyses of Atlantic salmon muscle genes induced by a DNA vaccine against salmonid alphavirus, the causative agent of salmon pancreas disease (PD). |
title_short |
Transcriptome analyses of Atlantic salmon muscle genes induced by a DNA vaccine against salmonid alphavirus, the causative agent of salmon pancreas disease (PD). |
title_full |
Transcriptome analyses of Atlantic salmon muscle genes induced by a DNA vaccine against salmonid alphavirus, the causative agent of salmon pancreas disease (PD). |
title_fullStr |
Transcriptome analyses of Atlantic salmon muscle genes induced by a DNA vaccine against salmonid alphavirus, the causative agent of salmon pancreas disease (PD). |
title_full_unstemmed |
Transcriptome analyses of Atlantic salmon muscle genes induced by a DNA vaccine against salmonid alphavirus, the causative agent of salmon pancreas disease (PD). |
title_sort |
transcriptome analyses of atlantic salmon muscle genes induced by a dna vaccine against salmonid alphavirus, the causative agent of salmon pancreas disease (pd). |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pone.0204924 https://doaj.org/article/8ee0c87ea99047118fbb10382bf0c5f0 |
long_lat |
ENVELOPE(156.400,156.400,68.817,68.817) |
geographic |
Sav’ |
geographic_facet |
Sav’ |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
PLoS ONE, Vol 13, Iss 10, p e0204924 (2018) |
op_relation |
http://europepmc.org/articles/PMC6166962?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0204924 https://doaj.org/article/8ee0c87ea99047118fbb10382bf0c5f0 |
op_doi |
https://doi.org/10.1371/journal.pone.0204924 |
container_title |
PLOS ONE |
container_volume |
13 |
container_issue |
10 |
container_start_page |
e0204924 |
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1766362303688605696 |