Antidiabetic activity of Callicarpa nudiflora extract in type 2 diabetic rats via activation of the AMPK-ACC pathway
Objective: To evaluate the antidiabetic effect of Callicarpa nudiflora extract in streptozotocin- induced diabetic rats. Methods: The chemical constituents in Callicarpa nudiflora extract were identified by UPLC- Q-TOF-MS. Callicarpa nudiflora extract (0.15 and 0.3 g/kg) was orally administered to s...
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Wolters Kluwer Medknow Publications
2019
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ftdoajarticles:oai:doaj.org/article:8e5f317aab224f90b696d156a3ca6a4c 2023-05-15T15:12:34+02:00 Antidiabetic activity of Callicarpa nudiflora extract in type 2 diabetic rats via activation of the AMPK-ACC pathway Wen-Yu Ma Le-Ping Ma Bo Yi Min Zhang Shi-Xiu Feng Li-Ping Tian 2019-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.270978 https://doaj.org/article/8e5f317aab224f90b696d156a3ca6a4c EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=11;spage=456;epage=466;aulast=Ma https://doaj.org/toc/2588-9222 2588-9222 doi:10.4103/2221-1691.270978 https://doaj.org/article/8e5f317aab224f90b696d156a3ca6a4c Asian Pacific Journal of Tropical Biomedicine, Vol 9, Iss 11, Pp 456-466 (2019) callicarpa nudiflora extract diabetes mellitus insulin resistance ampk-acc glut4 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2019 ftdoajarticles https://doi.org/10.4103/2221-1691.270978 2022-12-31T01:54:57Z Objective: To evaluate the antidiabetic effect of Callicarpa nudiflora extract in streptozotocin- induced diabetic rats. Methods: The chemical constituents in Callicarpa nudiflora extract were identified by UPLC- Q-TOF-MS. Callicarpa nudiflora extract (0.15 and 0.3 g/kg) was orally administered to streptozotocin-induced diabetic rats for 42 d. The effects of Callicarpa nudiflora extract on body weight, blood glucose, serum insulin, total cholesterol, triglyceride, LDL-C and HDL-C were investigated, and its effect on liver and pancreatic pathology was assessed by histopathological analysis. Moreover, the expression levels of adenosine 5’-monophosphate-activated protein kinase (AMPK), glucose transporter type 4 (GLUT4), phospho-AMPK/AMPK, and p-acetyl- coA carboxylase (P-ACC)/ACC in the skeletal muscles and liver were determined by reverse transcription-polymerase chain reaction, Western blotting, and immunohistochemistry. Results: A total of 34 compounds, including 8 iridoids, 14 phenylpropanoids, and 12 flavonoids, were identified from Callicarpa nudiflora extract. Callicarpa nudiflora extract significantly reduced blood glucose and significantly restored all other biochemical parameters to near normal levels, including serum insulin, total cholesterol, triglyceride, LDL-C, and HDL-C. Callicarpa nudiflora extract improved insulin resistance and reversed the damage in the liver and pancreas caused by diabetes. Furthermore, Callicarpa nudiflora extract increased the expression levels of phospho-AMPK, GLUT4, P-ACC, and insulin receptor substrate-1 and decreased the expression level of PPAR γ in diabetic rats. Conclusions: Callicarpa nudiflora extract improved oral glucose tolerance, lipid metabolism, insulin resistance, and reversed the diabetes-related damage in the liver and pancreas by activating the AMPK-ACC pathway. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 9 11 456 |
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English |
topic |
callicarpa nudiflora extract diabetes mellitus insulin resistance ampk-acc glut4 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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callicarpa nudiflora extract diabetes mellitus insulin resistance ampk-acc glut4 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Wen-Yu Ma Le-Ping Ma Bo Yi Min Zhang Shi-Xiu Feng Li-Ping Tian Antidiabetic activity of Callicarpa nudiflora extract in type 2 diabetic rats via activation of the AMPK-ACC pathway |
topic_facet |
callicarpa nudiflora extract diabetes mellitus insulin resistance ampk-acc glut4 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate the antidiabetic effect of Callicarpa nudiflora extract in streptozotocin- induced diabetic rats. Methods: The chemical constituents in Callicarpa nudiflora extract were identified by UPLC- Q-TOF-MS. Callicarpa nudiflora extract (0.15 and 0.3 g/kg) was orally administered to streptozotocin-induced diabetic rats for 42 d. The effects of Callicarpa nudiflora extract on body weight, blood glucose, serum insulin, total cholesterol, triglyceride, LDL-C and HDL-C were investigated, and its effect on liver and pancreatic pathology was assessed by histopathological analysis. Moreover, the expression levels of adenosine 5’-monophosphate-activated protein kinase (AMPK), glucose transporter type 4 (GLUT4), phospho-AMPK/AMPK, and p-acetyl- coA carboxylase (P-ACC)/ACC in the skeletal muscles and liver were determined by reverse transcription-polymerase chain reaction, Western blotting, and immunohistochemistry. Results: A total of 34 compounds, including 8 iridoids, 14 phenylpropanoids, and 12 flavonoids, were identified from Callicarpa nudiflora extract. Callicarpa nudiflora extract significantly reduced blood glucose and significantly restored all other biochemical parameters to near normal levels, including serum insulin, total cholesterol, triglyceride, LDL-C, and HDL-C. Callicarpa nudiflora extract improved insulin resistance and reversed the damage in the liver and pancreas caused by diabetes. Furthermore, Callicarpa nudiflora extract increased the expression levels of phospho-AMPK, GLUT4, P-ACC, and insulin receptor substrate-1 and decreased the expression level of PPAR γ in diabetic rats. Conclusions: Callicarpa nudiflora extract improved oral glucose tolerance, lipid metabolism, insulin resistance, and reversed the diabetes-related damage in the liver and pancreas by activating the AMPK-ACC pathway. |
format |
Article in Journal/Newspaper |
author |
Wen-Yu Ma Le-Ping Ma Bo Yi Min Zhang Shi-Xiu Feng Li-Ping Tian |
author_facet |
Wen-Yu Ma Le-Ping Ma Bo Yi Min Zhang Shi-Xiu Feng Li-Ping Tian |
author_sort |
Wen-Yu Ma |
title |
Antidiabetic activity of Callicarpa nudiflora extract in type 2 diabetic rats via activation of the AMPK-ACC pathway |
title_short |
Antidiabetic activity of Callicarpa nudiflora extract in type 2 diabetic rats via activation of the AMPK-ACC pathway |
title_full |
Antidiabetic activity of Callicarpa nudiflora extract in type 2 diabetic rats via activation of the AMPK-ACC pathway |
title_fullStr |
Antidiabetic activity of Callicarpa nudiflora extract in type 2 diabetic rats via activation of the AMPK-ACC pathway |
title_full_unstemmed |
Antidiabetic activity of Callicarpa nudiflora extract in type 2 diabetic rats via activation of the AMPK-ACC pathway |
title_sort |
antidiabetic activity of callicarpa nudiflora extract in type 2 diabetic rats via activation of the ampk-acc pathway |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2019 |
url |
https://doi.org/10.4103/2221-1691.270978 https://doaj.org/article/8e5f317aab224f90b696d156a3ca6a4c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 9, Iss 11, Pp 456-466 (2019) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=11;spage=456;epage=466;aulast=Ma https://doaj.org/toc/2588-9222 2588-9222 doi:10.4103/2221-1691.270978 https://doaj.org/article/8e5f317aab224f90b696d156a3ca6a4c |
op_doi |
https://doi.org/10.4103/2221-1691.270978 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
9 |
container_issue |
11 |
container_start_page |
456 |
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1766343221765472256 |