Tropheryma whipplei: a common bacterium in rural Senegal.
BACKGROUND: Tropheryma whipplei is known as the cause of Whipple's disease, but it is also an emerging pathogen, detected in stool, that causes various chronic localized infections without histological digestive involvement and is associated with acute infections, including gastroenteritis and...
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ftdoajarticles:oai:doaj.org/article:8e519916f4e240a3957cdb03b0ef0620 2023-05-15T15:14:13+02:00 Tropheryma whipplei: a common bacterium in rural Senegal. Alpha Kabinet Keita Hubert Bassene Adama Tall Cheikh Sokhna Pavel Ratmanov Jean-François Trape Didier Raoult Florence Fenollar 2011-12-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001403 https://doaj.org/article/8e519916f4e240a3957cdb03b0ef0620 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3243712?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001403 https://doaj.org/article/8e519916f4e240a3957cdb03b0ef0620 PLoS Neglected Tropical Diseases, Vol 5, Iss 12, p e1403 (2011) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2011 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001403 2022-12-31T13:00:01Z BACKGROUND: Tropheryma whipplei is known as the cause of Whipple's disease, but it is also an emerging pathogen, detected in stool, that causes various chronic localized infections without histological digestive involvement and is associated with acute infections, including gastroenteritis and bacteremia. METHODS/PRINCIPAL FINDINGS: We conducted a study in 2008 and 2009 using 497 non-diarrheic and diarrheic stool samples, 370 saliva samples, 454 sera samples and 105 samples obtained from water samples in two rural Sine-Saloum villages (Dielmo and Ndiop) in Senegal. The presence of T. whipplei was investigated by using specific quantitative PCR. Genotyping was performed on positive samples. A serological analysis by western blotting was performed to determine the seroprevalence and to detect seroconversion. Overall, T. whipplei was identified in 31.2% of the stool samples (139/446) and 3.5% of the saliva samples (13/370) obtained from healthy subjects. The carriage in the stool specimens was significantly (p<10(-3)) higher in children who were between 0 and 4 years old (60/80, 75%) compared to samples obtained from individuals who were between 5 to 10 years old (36/119, 30.2%) or between 11 and 99 years old (43/247, 17.4%). The carriage in the stool was also significantly more common (p = 0.015) in subjects with diarrhea (25/51, 49%). We identified 22 genotypes, 16 of which were new. Only one genotype (#53) was common to both villages. Among the specific genotypes, one (#52) was epidemic in Dielmo (15/28, 53.4%, p<10(-3)) and another (#49) in Ndiop (27.6%, p = 0.002). The overall seroprevalence was estimated at 72.8% (291/400). Seroconversion was detected in 66.7% (18/27) of children for whom PCR became positive in stools between 2008 and 2009. CONCLUSIONS/SIGNIFICANCE: T. whipplei is a common bacterium in the Sine-Saloum area of rural Senegal that is contracted early in childhood. Epidemic genotypes suggest a human transmission of the bacterium. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 5 12 e1403 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Alpha Kabinet Keita Hubert Bassene Adama Tall Cheikh Sokhna Pavel Ratmanov Jean-François Trape Didier Raoult Florence Fenollar Tropheryma whipplei: a common bacterium in rural Senegal. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: Tropheryma whipplei is known as the cause of Whipple's disease, but it is also an emerging pathogen, detected in stool, that causes various chronic localized infections without histological digestive involvement and is associated with acute infections, including gastroenteritis and bacteremia. METHODS/PRINCIPAL FINDINGS: We conducted a study in 2008 and 2009 using 497 non-diarrheic and diarrheic stool samples, 370 saliva samples, 454 sera samples and 105 samples obtained from water samples in two rural Sine-Saloum villages (Dielmo and Ndiop) in Senegal. The presence of T. whipplei was investigated by using specific quantitative PCR. Genotyping was performed on positive samples. A serological analysis by western blotting was performed to determine the seroprevalence and to detect seroconversion. Overall, T. whipplei was identified in 31.2% of the stool samples (139/446) and 3.5% of the saliva samples (13/370) obtained from healthy subjects. The carriage in the stool specimens was significantly (p<10(-3)) higher in children who were between 0 and 4 years old (60/80, 75%) compared to samples obtained from individuals who were between 5 to 10 years old (36/119, 30.2%) or between 11 and 99 years old (43/247, 17.4%). The carriage in the stool was also significantly more common (p = 0.015) in subjects with diarrhea (25/51, 49%). We identified 22 genotypes, 16 of which were new. Only one genotype (#53) was common to both villages. Among the specific genotypes, one (#52) was epidemic in Dielmo (15/28, 53.4%, p<10(-3)) and another (#49) in Ndiop (27.6%, p = 0.002). The overall seroprevalence was estimated at 72.8% (291/400). Seroconversion was detected in 66.7% (18/27) of children for whom PCR became positive in stools between 2008 and 2009. CONCLUSIONS/SIGNIFICANCE: T. whipplei is a common bacterium in the Sine-Saloum area of rural Senegal that is contracted early in childhood. Epidemic genotypes suggest a human transmission of the bacterium. |
format |
Article in Journal/Newspaper |
author |
Alpha Kabinet Keita Hubert Bassene Adama Tall Cheikh Sokhna Pavel Ratmanov Jean-François Trape Didier Raoult Florence Fenollar |
author_facet |
Alpha Kabinet Keita Hubert Bassene Adama Tall Cheikh Sokhna Pavel Ratmanov Jean-François Trape Didier Raoult Florence Fenollar |
author_sort |
Alpha Kabinet Keita |
title |
Tropheryma whipplei: a common bacterium in rural Senegal. |
title_short |
Tropheryma whipplei: a common bacterium in rural Senegal. |
title_full |
Tropheryma whipplei: a common bacterium in rural Senegal. |
title_fullStr |
Tropheryma whipplei: a common bacterium in rural Senegal. |
title_full_unstemmed |
Tropheryma whipplei: a common bacterium in rural Senegal. |
title_sort |
tropheryma whipplei: a common bacterium in rural senegal. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2011 |
url |
https://doi.org/10.1371/journal.pntd.0001403 https://doaj.org/article/8e519916f4e240a3957cdb03b0ef0620 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 5, Iss 12, p e1403 (2011) |
op_relation |
http://europepmc.org/articles/PMC3243712?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001403 https://doaj.org/article/8e519916f4e240a3957cdb03b0ef0620 |
op_doi |
https://doi.org/10.1371/journal.pntd.0001403 |
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PLoS Neglected Tropical Diseases |
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5 |
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12 |
container_start_page |
e1403 |
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1766344694651944960 |