Heterologous expression and mutagenesis of recombinant Vespa affinis hyaluronidase protein (rVesA2)

• Published in: Journal of Venomous Animals and Toxins including Tropical Diseases
• Main Authors: Prapenpuksiri Rungsa, Piyapon Janpan, Yutthakan Saengkun, Nisachon Jangpromma, Sompong Klaynongsruang, Rina Patramanon, Nunthawun Uawonggul, Jureerut Daduang, Sakda Daduang
• Format: Article in Journal/Newspaper
• Language: English
• Published: SciELO 2019
• Subjects: Vespa affinis; Hyaluronidase; Wasp; Venom; Structure analysis; Modelling; Cloning; Protein expression; Arctic medicine. Tropical medicine; RC955-962; Toxicology. Poisons; RA1190-1270; Zoology; QL1-991; Arctic; Double Point
• Online Access: https://doi.org/10.1590/1678-9199-jvatitd-2019-0030; https://doaj.org/article/8e27f3a3196a4c8eb0aea9e70eee7f21

Abstract Background: Crude venom of the banded tiger waspVespa affinis contains a variety of enzymes including hyaluronidases, commonly known as spreading factors. Methods: The cDNA cloning, sequence analysis and structural modelling of V. affinis venom hyaluronidase (VesA2) were herein described. M...