Interaction between dietary fatty acids and genotype on immune response in Atlantic salmon (Salmo salar) after vaccination: A transcriptome study.
A pivotal matter to aquaculture is the sourcing of sustainable resources as ingredients to aquafeeds. Levels of plant delivered oils as source of fatty acids (FA) in aquafeeds have reached around 70% resulting in reduced levels of long-chain omega-3 polyunsaturated fatty acids (LC n-3 PUFA), such as...
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ftdoajarticles:oai:doaj.org/article:8e0c399bcb574c0e9b243ac8a6f9d902 2023-05-15T15:30:41+02:00 Interaction between dietary fatty acids and genotype on immune response in Atlantic salmon (Salmo salar) after vaccination: A transcriptome study. Adriana Magalhães Santos Andresen Esmail Lutfi Bente Ruyter Gerd Berge Tor Gjøen 2019-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0219625 https://doaj.org/article/8e0c399bcb574c0e9b243ac8a6f9d902 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pone.0219625 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0219625 https://doaj.org/article/8e0c399bcb574c0e9b243ac8a6f9d902 PLoS ONE, Vol 14, Iss 7, p e0219625 (2019) Medicine R Science Q article 2019 ftdoajarticles https://doi.org/10.1371/journal.pone.0219625 2022-12-31T05:56:41Z A pivotal matter to aquaculture is the sourcing of sustainable resources as ingredients to aquafeeds. Levels of plant delivered oils as source of fatty acids (FA) in aquafeeds have reached around 70% resulting in reduced levels of long-chain omega-3 polyunsaturated fatty acids (LC n-3 PUFA), such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), in salmon fillet composition. EPA and DHA can modulate inflammation and immune response, so it is crucial to understand how fish immune response is affected by low LC n-3 PUFA diet and if this diet can have a detrimental effect on vaccine response. Atlantic salmon (Salmo salar) can produce EPA/DHA from α-linolenic acid (ALA) and this endogenous capacity can be explored to develop families with higher tolerance to low LC n-3 PUFA diets. Here we analyze innate and adaptive immune response in Atlantic salmon to a commercial vaccine after being fed low levels of EPA and DHA, and we also compare three strains of salmon selected by their endogenous capacity of synthesizing LC- n-3 PUFA. A total of 2,890 differentially expressed genes (DEGs) were identified (p-value adjusted < 0.1) when comparing vaccinated fish against control non-vaccinated. Gene ontology (GO) and KEGG analysis with 442 up/downregulated genes revealed that most DEGs were both related to immune response as well as part of important immune related pathways, as "Toll-like receptor" and "Cytokine-Cytokine interaction". Adaptive response was also addressed by measuring antigen specific IgM, and titers were significantly higher than in the pre-immune fish at 62 days post-immunization. However, diet and strain had no/little effect on vaccine-specific IgM or innate immune responses. Atlantic salmon therefore display robustness in its response to vaccination even when feed low levels of LC n-3 PUFA. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles PLOS ONE 14 7 e0219625 |
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Medicine R Science Q |
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Medicine R Science Q Adriana Magalhães Santos Andresen Esmail Lutfi Bente Ruyter Gerd Berge Tor Gjøen Interaction between dietary fatty acids and genotype on immune response in Atlantic salmon (Salmo salar) after vaccination: A transcriptome study. |
topic_facet |
Medicine R Science Q |
description |
A pivotal matter to aquaculture is the sourcing of sustainable resources as ingredients to aquafeeds. Levels of plant delivered oils as source of fatty acids (FA) in aquafeeds have reached around 70% resulting in reduced levels of long-chain omega-3 polyunsaturated fatty acids (LC n-3 PUFA), such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), in salmon fillet composition. EPA and DHA can modulate inflammation and immune response, so it is crucial to understand how fish immune response is affected by low LC n-3 PUFA diet and if this diet can have a detrimental effect on vaccine response. Atlantic salmon (Salmo salar) can produce EPA/DHA from α-linolenic acid (ALA) and this endogenous capacity can be explored to develop families with higher tolerance to low LC n-3 PUFA diets. Here we analyze innate and adaptive immune response in Atlantic salmon to a commercial vaccine after being fed low levels of EPA and DHA, and we also compare three strains of salmon selected by their endogenous capacity of synthesizing LC- n-3 PUFA. A total of 2,890 differentially expressed genes (DEGs) were identified (p-value adjusted < 0.1) when comparing vaccinated fish against control non-vaccinated. Gene ontology (GO) and KEGG analysis with 442 up/downregulated genes revealed that most DEGs were both related to immune response as well as part of important immune related pathways, as "Toll-like receptor" and "Cytokine-Cytokine interaction". Adaptive response was also addressed by measuring antigen specific IgM, and titers were significantly higher than in the pre-immune fish at 62 days post-immunization. However, diet and strain had no/little effect on vaccine-specific IgM or innate immune responses. Atlantic salmon therefore display robustness in its response to vaccination even when feed low levels of LC n-3 PUFA. |
format |
Article in Journal/Newspaper |
author |
Adriana Magalhães Santos Andresen Esmail Lutfi Bente Ruyter Gerd Berge Tor Gjøen |
author_facet |
Adriana Magalhães Santos Andresen Esmail Lutfi Bente Ruyter Gerd Berge Tor Gjøen |
author_sort |
Adriana Magalhães Santos Andresen |
title |
Interaction between dietary fatty acids and genotype on immune response in Atlantic salmon (Salmo salar) after vaccination: A transcriptome study. |
title_short |
Interaction between dietary fatty acids and genotype on immune response in Atlantic salmon (Salmo salar) after vaccination: A transcriptome study. |
title_full |
Interaction between dietary fatty acids and genotype on immune response in Atlantic salmon (Salmo salar) after vaccination: A transcriptome study. |
title_fullStr |
Interaction between dietary fatty acids and genotype on immune response in Atlantic salmon (Salmo salar) after vaccination: A transcriptome study. |
title_full_unstemmed |
Interaction between dietary fatty acids and genotype on immune response in Atlantic salmon (Salmo salar) after vaccination: A transcriptome study. |
title_sort |
interaction between dietary fatty acids and genotype on immune response in atlantic salmon (salmo salar) after vaccination: a transcriptome study. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pone.0219625 https://doaj.org/article/8e0c399bcb574c0e9b243ac8a6f9d902 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
PLoS ONE, Vol 14, Iss 7, p e0219625 (2019) |
op_relation |
https://doi.org/10.1371/journal.pone.0219625 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0219625 https://doaj.org/article/8e0c399bcb574c0e9b243ac8a6f9d902 |
op_doi |
https://doi.org/10.1371/journal.pone.0219625 |
container_title |
PLOS ONE |
container_volume |
14 |
container_issue |
7 |
container_start_page |
e0219625 |
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1766361129300262912 |