Protein extract of kenaf seed exhibits anticoagulant, antiplatelet and antioxidant activities
Objective: To explore the anticoagulant, antiplatelet and antioxidant activities of protein extract of kenaf seed (PEKS). Methods: Sodium dodecyl sulphate polyacrylamide gel electrophoresis and reverse-phase high-performance liquid chromatography techniques were employed for protein characterization...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2022
|
Subjects: | |
Online Access: | https://doi.org/10.4103/2221-1691.335693 https://doaj.org/article/8ddf12d9b7d6411586bc3fc41ffb1e51 |
id |
ftdoajarticles:oai:doaj.org/article:8ddf12d9b7d6411586bc3fc41ffb1e51 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:8ddf12d9b7d6411586bc3fc41ffb1e51 2023-05-15T15:09:54+02:00 Protein extract of kenaf seed exhibits anticoagulant, antiplatelet and antioxidant activities Sujatha M Hanumegowda Chandramma Srinivasa Ashwini Shivaiah Manjula M Venkatappa Ramesha Hanumanthappa Rajesh Rangappa Ramesh K Laxmaiah Sathisha J Gonchigar Devaraja Sannaningaiah 2022-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.335693 https://doaj.org/article/8ddf12d9b7d6411586bc3fc41ffb1e51 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=2;spage=47;epage=58;aulast=Hanumegowda https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.335693 https://doaj.org/article/8ddf12d9b7d6411586bc3fc41ffb1e51 Asian Pacific Journal of Tropical Biomedicine, Vol 12, Iss 2, Pp 47-58 (2022) protein extract kenaf seed oxidative stress antioxidant anticoagulant antiplatelet Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.4103/2221-1691.335693 2022-12-31T14:41:04Z Objective: To explore the anticoagulant, antiplatelet and antioxidant activities of protein extract of kenaf seed (PEKS). Methods: Sodium dodecyl sulphate polyacrylamide gel electrophoresis and reverse-phase high-performance liquid chromatography techniques were employed for protein characterization. Antioxidant activity of PEKS was assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The protective effect of PEKS on sodium nitrite (NaNO2) induced oxidative stress was evaluated using the in vitro red blood cell model, while the effect of PEKS on diclofenac-induced oxidative stress was examined in vivo in rats. Platelet-rich plasma and platelet-poor plasma were used for anticoagulant and antiplatelet activities of PEKS. Results: PEKS revealed similar protein bands on SDS-PAGE under reduced and non-reduced conditions. Several acidic proteins were present in native PAGE. PEKS showed antioxidant properties by scavenging DPPH with an IC50 of 24.58 μg. PEKS exhibited a protective effect on NaNO2 induced oxidative stress in red blood cells by restoring the activity of stress markers. In addition, PEKS alleviated diclofenac-induced tissue damage of the liver, kidney, and small intestine. PEKS showed an anticoagulant effect in both in vivo and in vitro experiments by enhancing normal clotting time. PEKS did not affect prothrombin time but increase activated partial thromboplastin time. Furthermore, PEKS inhibited adenosine diphosphate and epinephrine-induced platelet aggregation. Conclusions: PEKS protects tissues from oxidative stress and exhibits antithrombotic activity. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 12 2 47 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
protein extract kenaf seed oxidative stress antioxidant anticoagulant antiplatelet Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
protein extract kenaf seed oxidative stress antioxidant anticoagulant antiplatelet Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Sujatha M Hanumegowda Chandramma Srinivasa Ashwini Shivaiah Manjula M Venkatappa Ramesha Hanumanthappa Rajesh Rangappa Ramesh K Laxmaiah Sathisha J Gonchigar Devaraja Sannaningaiah Protein extract of kenaf seed exhibits anticoagulant, antiplatelet and antioxidant activities |
topic_facet |
protein extract kenaf seed oxidative stress antioxidant anticoagulant antiplatelet Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To explore the anticoagulant, antiplatelet and antioxidant activities of protein extract of kenaf seed (PEKS). Methods: Sodium dodecyl sulphate polyacrylamide gel electrophoresis and reverse-phase high-performance liquid chromatography techniques were employed for protein characterization. Antioxidant activity of PEKS was assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The protective effect of PEKS on sodium nitrite (NaNO2) induced oxidative stress was evaluated using the in vitro red blood cell model, while the effect of PEKS on diclofenac-induced oxidative stress was examined in vivo in rats. Platelet-rich plasma and platelet-poor plasma were used for anticoagulant and antiplatelet activities of PEKS. Results: PEKS revealed similar protein bands on SDS-PAGE under reduced and non-reduced conditions. Several acidic proteins were present in native PAGE. PEKS showed antioxidant properties by scavenging DPPH with an IC50 of 24.58 μg. PEKS exhibited a protective effect on NaNO2 induced oxidative stress in red blood cells by restoring the activity of stress markers. In addition, PEKS alleviated diclofenac-induced tissue damage of the liver, kidney, and small intestine. PEKS showed an anticoagulant effect in both in vivo and in vitro experiments by enhancing normal clotting time. PEKS did not affect prothrombin time but increase activated partial thromboplastin time. Furthermore, PEKS inhibited adenosine diphosphate and epinephrine-induced platelet aggregation. Conclusions: PEKS protects tissues from oxidative stress and exhibits antithrombotic activity. |
format |
Article in Journal/Newspaper |
author |
Sujatha M Hanumegowda Chandramma Srinivasa Ashwini Shivaiah Manjula M Venkatappa Ramesha Hanumanthappa Rajesh Rangappa Ramesh K Laxmaiah Sathisha J Gonchigar Devaraja Sannaningaiah |
author_facet |
Sujatha M Hanumegowda Chandramma Srinivasa Ashwini Shivaiah Manjula M Venkatappa Ramesha Hanumanthappa Rajesh Rangappa Ramesh K Laxmaiah Sathisha J Gonchigar Devaraja Sannaningaiah |
author_sort |
Sujatha M Hanumegowda |
title |
Protein extract of kenaf seed exhibits anticoagulant, antiplatelet and antioxidant activities |
title_short |
Protein extract of kenaf seed exhibits anticoagulant, antiplatelet and antioxidant activities |
title_full |
Protein extract of kenaf seed exhibits anticoagulant, antiplatelet and antioxidant activities |
title_fullStr |
Protein extract of kenaf seed exhibits anticoagulant, antiplatelet and antioxidant activities |
title_full_unstemmed |
Protein extract of kenaf seed exhibits anticoagulant, antiplatelet and antioxidant activities |
title_sort |
protein extract of kenaf seed exhibits anticoagulant, antiplatelet and antioxidant activities |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2022 |
url |
https://doi.org/10.4103/2221-1691.335693 https://doaj.org/article/8ddf12d9b7d6411586bc3fc41ffb1e51 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 12, Iss 2, Pp 47-58 (2022) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=2;spage=47;epage=58;aulast=Hanumegowda https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.335693 https://doaj.org/article/8ddf12d9b7d6411586bc3fc41ffb1e51 |
op_doi |
https://doi.org/10.4103/2221-1691.335693 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
12 |
container_issue |
2 |
container_start_page |
47 |
_version_ |
1766340992298909696 |