Anti-Inflammatory Effects Exerted by 14-Methoxyalternate C from Antarctic Fungal Strain Pleosporales sp. SF-7343 via the Regulation of NF-κB and JAK2/STAT3 in HaCaT Human Keratinocytes

• Published in: International Journal of Molecular Sciences
• Main Authors: Linsha Dong, Thao Quyen Cao, Zhiming Liu, Nguyen Quoc Tuan, Youn-Chul Kim, Jae Hak Sohn, Joung Han Yim, Dong-Sung Lee, Hyuncheol Oh
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI AG 2022
• Subjects: Antarctic fungi; skin inflammation; JAK2/STAT3; NF-κB; HaCaT; Biology (General); QH301-705.5; Chemistry; QD1-999; Antarctic; The Antarctic; Antarc*
• Online Access: https://doi.org/10.3390/ijms232314642; https://doaj.org/article/8dd76bb74be84d0a82b958cdf3af9149

Atopic dermatitis (AD) is a chronic inflammatory skin disease with a profound negative impact on patients’ quality of life. Four known secondary fungal metabolites were found in the chemical study of the Antarctic fungus Pleosporales sp. SF-7343, including 14-methoxyalternate C ( 1 ), 5′-methoxy-6-methyl-biphenyl-3,4,3′-triol ( 2 ), 3,8,10-trihydroxy-4-methoxy-6-methylbenzocoumarin ( 3 ), and alternariol monomethyl ether ( 4 ). Additionally, we identified the skin anti-inflammatory composition from the SF-7343 strain. Interleukin-8 and -6 Screening results showed that compound 1 inhibited IL-8 and IL-6 in tumor necrosis factor-α/interferon-γ stimulated HaCaT cells. Compound 1 showed inhibitory effects on MDC and RANTES. It also downregulated the expression of intercellular adhesion molecule-1 (ICAM-1) and upregulated the expression of involucrin. The results of the mechanistic study showed that compound 1 inhibited the nuclear translocation of nuclear factor-kappa B p65 and STAT3. In conclusion, this study demonstrates the potential of the Antarctic fungal strain SF-7343 as a bioactive resource to inhibit skin inflammation, such as AD.