Screening strategies and laboratory assays to support Plasmodium falciparum histidine-rich protein deletion surveillance: where we are and what is needed
Abstract Rapid diagnostic tests (RDTs) detecting Plasmodium falciparum histidine-rich protein 2 (HRP2) have been an important tool for malaria diagnosis, especially in resource-limited settings lacking quality microscopy. Plasmodium falciparum parasites with deletion of the pfhrp2 gene encoding this...
Published in: | Malaria Journal |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2022
|
Subjects: | |
Online Access: | https://doi.org/10.1186/s12936-022-04226-2 https://doaj.org/article/8dd54b7169c74c5c9f2e77594a223096 |
id |
ftdoajarticles:oai:doaj.org/article:8dd54b7169c74c5c9f2e77594a223096 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:8dd54b7169c74c5c9f2e77594a223096 2023-05-15T15:13:15+02:00 Screening strategies and laboratory assays to support Plasmodium falciparum histidine-rich protein deletion surveillance: where we are and what is needed Khalid B. Beshir Jonathan B. Parr Jane Cunningham Qin Cheng Eric Rogier 2022-06-01T00:00:00Z https://doi.org/10.1186/s12936-022-04226-2 https://doaj.org/article/8dd54b7169c74c5c9f2e77594a223096 EN eng BMC https://doi.org/10.1186/s12936-022-04226-2 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-022-04226-2 1475-2875 https://doaj.org/article/8dd54b7169c74c5c9f2e77594a223096 Malaria Journal, Vol 21, Iss 1, Pp 1-12 (2022) Malaria Rapid diagnostic test pfhrp2 pfhrp3 Gene deletions Laboratory assay Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2022 ftdoajarticles https://doi.org/10.1186/s12936-022-04226-2 2022-12-31T01:53:08Z Abstract Rapid diagnostic tests (RDTs) detecting Plasmodium falciparum histidine-rich protein 2 (HRP2) have been an important tool for malaria diagnosis, especially in resource-limited settings lacking quality microscopy. Plasmodium falciparum parasites with deletion of the pfhrp2 gene encoding this antigen have now been identified in dozens of countries across Asia, Africa, and South America, with new reports revealing a high prevalence of deletions in some selected regions. To determine whether HRP2-based RDTs are appropriate for continued use in a locality, focused surveys and/or surveillance activities of the endemic P. falciparum population are needed. Various survey and laboratory methods have been used to determine parasite HRP2 phenotype and pfhrp2 genotype, and the data collected by these different methods need to be interpreted in the appropriate context of survey and assay utilized. Expression of the HRP2 antigen can be evaluated using point-of-care RDTs or laboratory-based immunoassays, but confirmation of a deletion (or mutation) of pfhrp2 requires more intensive laboratory molecular assays, and new tools and strategies for rigorous but practical data collection are particularly needed for large surveys. Because malaria diagnostic strategies are typically developed at the national level, nationally representative surveys and/or surveillance that encompass broad geographical areas and large populations may be required. Here is discussed contemporary assays for the phenotypic and genotypic evaluation of P. falciparum HRP2 status, consider their strengths and weaknesses, and highlight key concepts relevant to timely and resource-conscious workflows required for efficient diagnostic policy decision making. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 21 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Malaria Rapid diagnostic test pfhrp2 pfhrp3 Gene deletions Laboratory assay Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria Rapid diagnostic test pfhrp2 pfhrp3 Gene deletions Laboratory assay Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Khalid B. Beshir Jonathan B. Parr Jane Cunningham Qin Cheng Eric Rogier Screening strategies and laboratory assays to support Plasmodium falciparum histidine-rich protein deletion surveillance: where we are and what is needed |
topic_facet |
Malaria Rapid diagnostic test pfhrp2 pfhrp3 Gene deletions Laboratory assay Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Rapid diagnostic tests (RDTs) detecting Plasmodium falciparum histidine-rich protein 2 (HRP2) have been an important tool for malaria diagnosis, especially in resource-limited settings lacking quality microscopy. Plasmodium falciparum parasites with deletion of the pfhrp2 gene encoding this antigen have now been identified in dozens of countries across Asia, Africa, and South America, with new reports revealing a high prevalence of deletions in some selected regions. To determine whether HRP2-based RDTs are appropriate for continued use in a locality, focused surveys and/or surveillance activities of the endemic P. falciparum population are needed. Various survey and laboratory methods have been used to determine parasite HRP2 phenotype and pfhrp2 genotype, and the data collected by these different methods need to be interpreted in the appropriate context of survey and assay utilized. Expression of the HRP2 antigen can be evaluated using point-of-care RDTs or laboratory-based immunoassays, but confirmation of a deletion (or mutation) of pfhrp2 requires more intensive laboratory molecular assays, and new tools and strategies for rigorous but practical data collection are particularly needed for large surveys. Because malaria diagnostic strategies are typically developed at the national level, nationally representative surveys and/or surveillance that encompass broad geographical areas and large populations may be required. Here is discussed contemporary assays for the phenotypic and genotypic evaluation of P. falciparum HRP2 status, consider their strengths and weaknesses, and highlight key concepts relevant to timely and resource-conscious workflows required for efficient diagnostic policy decision making. |
format |
Article in Journal/Newspaper |
author |
Khalid B. Beshir Jonathan B. Parr Jane Cunningham Qin Cheng Eric Rogier |
author_facet |
Khalid B. Beshir Jonathan B. Parr Jane Cunningham Qin Cheng Eric Rogier |
author_sort |
Khalid B. Beshir |
title |
Screening strategies and laboratory assays to support Plasmodium falciparum histidine-rich protein deletion surveillance: where we are and what is needed |
title_short |
Screening strategies and laboratory assays to support Plasmodium falciparum histidine-rich protein deletion surveillance: where we are and what is needed |
title_full |
Screening strategies and laboratory assays to support Plasmodium falciparum histidine-rich protein deletion surveillance: where we are and what is needed |
title_fullStr |
Screening strategies and laboratory assays to support Plasmodium falciparum histidine-rich protein deletion surveillance: where we are and what is needed |
title_full_unstemmed |
Screening strategies and laboratory assays to support Plasmodium falciparum histidine-rich protein deletion surveillance: where we are and what is needed |
title_sort |
screening strategies and laboratory assays to support plasmodium falciparum histidine-rich protein deletion surveillance: where we are and what is needed |
publisher |
BMC |
publishDate |
2022 |
url |
https://doi.org/10.1186/s12936-022-04226-2 https://doaj.org/article/8dd54b7169c74c5c9f2e77594a223096 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 21, Iss 1, Pp 1-12 (2022) |
op_relation |
https://doi.org/10.1186/s12936-022-04226-2 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-022-04226-2 1475-2875 https://doaj.org/article/8dd54b7169c74c5c9f2e77594a223096 |
op_doi |
https://doi.org/10.1186/s12936-022-04226-2 |
container_title |
Malaria Journal |
container_volume |
21 |
container_issue |
1 |
_version_ |
1766343824824598528 |