Use of immunoblotting assay improves the sensitivity of paracoccidioidomycosis diagnosis
The purpose of this work was to evaluate two serological assays: double immunodiffusion (DI) and immunoblotting (IB) in immunodiagnosis of paracoccidioidomycosis (PCM). We evaluated by IB assay 23 sera samples from patients with clinical confirmation of PCM, all of them with negative DI results agai...
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ftdoajarticles:oai:doaj.org/article:8dc77bdddf0840e78b54e274073b1ea2 2023-05-15T15:06:07+02:00 Use of immunoblotting assay improves the sensitivity of paracoccidioidomycosis diagnosis D. F. Silva C. M. Assis I. M. Zamboni L. C. Barreto V. S. Kohara A. P. Vicentini-Moreira 2008-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992008000200009 https://doaj.org/article/8dc77bdddf0840e78b54e274073b1ea2 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200009 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992008000200009 1678-9199 https://doaj.org/article/8dc77bdddf0840e78b54e274073b1ea2 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 14, Iss 2, Pp 313-321 (2008) paracoccidioidomycosis immunodiffusion immunoblotting Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2008 ftdoajarticles https://doi.org/10.1590/S1678-91992008000200009 2022-12-31T13:24:32Z The purpose of this work was to evaluate two serological assays: double immunodiffusion (DI) and immunoblotting (IB) in immunodiagnosis of paracoccidioidomycosis (PCM). We evaluated by IB assay 23 sera samples from patients with clinical confirmation of PCM, all of them with negative DI results against culture filtrate from Paracoccidioides brasiliensis isolate 113. For IB, as well as for comparative DI assay, we employed soluble components of the cell wall outer surface (SCCWOS) from P. brasiliensis isolate 113 cultivated at 36°C in Fava-Neto's agar medium for 5 and 10 days. Among the 20 sera samples analyzed by DI, 13 (65%) were negative and 7 (35%) were positive against SCCWOS obtained on the 5th and 10th days. By IB assay, 95.4% and 100% of sera reacted against gp43 and gp70 present in SCCWOS from the 5th day and 95.6% recognized these fractions when evaluated against SCCWOS from the 10th day. Our results demonstrated that the use of an immunoenzymatic assay significantly improves the sensitivity of PCM immunodiagnosis and also suggests that at least two serological tests for antibody detection should be adopted in cases of questionable diagnosis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 14 2 313 321 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
paracoccidioidomycosis immunodiffusion immunoblotting Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
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paracoccidioidomycosis immunodiffusion immunoblotting Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 D. F. Silva C. M. Assis I. M. Zamboni L. C. Barreto V. S. Kohara A. P. Vicentini-Moreira Use of immunoblotting assay improves the sensitivity of paracoccidioidomycosis diagnosis |
topic_facet |
paracoccidioidomycosis immunodiffusion immunoblotting Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
The purpose of this work was to evaluate two serological assays: double immunodiffusion (DI) and immunoblotting (IB) in immunodiagnosis of paracoccidioidomycosis (PCM). We evaluated by IB assay 23 sera samples from patients with clinical confirmation of PCM, all of them with negative DI results against culture filtrate from Paracoccidioides brasiliensis isolate 113. For IB, as well as for comparative DI assay, we employed soluble components of the cell wall outer surface (SCCWOS) from P. brasiliensis isolate 113 cultivated at 36°C in Fava-Neto's agar medium for 5 and 10 days. Among the 20 sera samples analyzed by DI, 13 (65%) were negative and 7 (35%) were positive against SCCWOS obtained on the 5th and 10th days. By IB assay, 95.4% and 100% of sera reacted against gp43 and gp70 present in SCCWOS from the 5th day and 95.6% recognized these fractions when evaluated against SCCWOS from the 10th day. Our results demonstrated that the use of an immunoenzymatic assay significantly improves the sensitivity of PCM immunodiagnosis and also suggests that at least two serological tests for antibody detection should be adopted in cases of questionable diagnosis. |
format |
Article in Journal/Newspaper |
author |
D. F. Silva C. M. Assis I. M. Zamboni L. C. Barreto V. S. Kohara A. P. Vicentini-Moreira |
author_facet |
D. F. Silva C. M. Assis I. M. Zamboni L. C. Barreto V. S. Kohara A. P. Vicentini-Moreira |
author_sort |
D. F. Silva |
title |
Use of immunoblotting assay improves the sensitivity of paracoccidioidomycosis diagnosis |
title_short |
Use of immunoblotting assay improves the sensitivity of paracoccidioidomycosis diagnosis |
title_full |
Use of immunoblotting assay improves the sensitivity of paracoccidioidomycosis diagnosis |
title_fullStr |
Use of immunoblotting assay improves the sensitivity of paracoccidioidomycosis diagnosis |
title_full_unstemmed |
Use of immunoblotting assay improves the sensitivity of paracoccidioidomycosis diagnosis |
title_sort |
use of immunoblotting assay improves the sensitivity of paracoccidioidomycosis diagnosis |
publisher |
SciELO |
publishDate |
2008 |
url |
https://doi.org/10.1590/S1678-91992008000200009 https://doaj.org/article/8dc77bdddf0840e78b54e274073b1ea2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 14, Iss 2, Pp 313-321 (2008) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200009 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992008000200009 1678-9199 https://doaj.org/article/8dc77bdddf0840e78b54e274073b1ea2 |
op_doi |
https://doi.org/10.1590/S1678-91992008000200009 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
14 |
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2 |
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313 |
op_container_end_page |
321 |
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1766337781188001792 |