Comparative transcriptome analysis of the newly discovered insect vector of the pine wood nematode in China, revealing putative genes related to host plant adaptation
Abstract Background In many insect species, the larvae/nymphs are unable to disperse far from the oviposition site selected by adults. The Sakhalin pine sawyer Monochamus saltuarius (Gebler) is the newly discovered insect vector of the pine wood nematode (Bursaphelenchus xylophilus) in China. Adult...
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ftdoajarticles:oai:doaj.org/article:8dc19aff78164a4f90dbd56b1e8bd6e5 2023-05-15T18:09:16+02:00 Comparative transcriptome analysis of the newly discovered insect vector of the pine wood nematode in China, revealing putative genes related to host plant adaptation Zehai Hou Fengming Shi Sixun Ge Jing Tao Lili Ren Hao Wu Shixiang Zong 2021-03-01T00:00:00Z https://doi.org/10.1186/s12864-021-07498-1 https://doaj.org/article/8dc19aff78164a4f90dbd56b1e8bd6e5 EN eng BMC https://doi.org/10.1186/s12864-021-07498-1 https://doaj.org/toc/1471-2164 doi:10.1186/s12864-021-07498-1 1471-2164 https://doaj.org/article/8dc19aff78164a4f90dbd56b1e8bd6e5 BMC Genomics, Vol 22, Iss 1, Pp 1-16 (2021) Cerambycidae Monochamus saltuarius Host adaptation Transcriptional variation Pinus koraiensis Pinus tabuliformis Biotechnology TP248.13-248.65 Genetics QH426-470 article 2021 ftdoajarticles https://doi.org/10.1186/s12864-021-07498-1 2022-12-31T12:56:53Z Abstract Background In many insect species, the larvae/nymphs are unable to disperse far from the oviposition site selected by adults. The Sakhalin pine sawyer Monochamus saltuarius (Gebler) is the newly discovered insect vector of the pine wood nematode (Bursaphelenchus xylophilus) in China. Adult M. saltuarius prefers to oviposit on the host plant Pinus koraiensis, rather than P. tabuliformis. However, the genetic basis of adaptation of the larvae of M. saltuarius with weaken dispersal ability to host environments selected by the adult is not well understood. Results In this study, the free amino and fatty acid composition and content of the host plants of M. saltuarius larvae, i.e., P. koraiensis and P. tabuliformis were investigated. Compared with P. koraiensis, P. tabuliformis had a substantially higher content of various free amino acids, while the opposite trend was detected for fatty acid content. The transcriptional profiles of larval populations feeding on P. koraiensis and P. tabuliformis were compared using PacBio Sequel II sequencing combined with Illumina sequencing. The results showed that genes relating to digestion, fatty acid synthesis, detoxification, oxidation-reduction, and stress response, as well as nutrients and energy sensing ability, were differentially expressed, possibly reflecting adaptive changes of M. saltuarius in response to different host diets. Additionally, genes coding for cuticle structure were differentially expressed, indicating that cuticle may be a potential target for plant defense. Differential regulation of genes related to the antibacterial and immune response were also observed, suggesting that larvae of M. saltuarius may have evolved adaptations to cope with bacterial challenges in their host environments. Conclusions The present study provides comprehensive transcriptome resource of M. saltuarius relating to host plant adaptation. Results from this study help to illustrate the fundamental relationship between transcriptional plasticity and adaptation mechanisms of ... Article in Journal/Newspaper Sakhalin Directory of Open Access Journals: DOAJ Articles BMC Genomics 22 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Cerambycidae Monochamus saltuarius Host adaptation Transcriptional variation Pinus koraiensis Pinus tabuliformis Biotechnology TP248.13-248.65 Genetics QH426-470 |
spellingShingle |
Cerambycidae Monochamus saltuarius Host adaptation Transcriptional variation Pinus koraiensis Pinus tabuliformis Biotechnology TP248.13-248.65 Genetics QH426-470 Zehai Hou Fengming Shi Sixun Ge Jing Tao Lili Ren Hao Wu Shixiang Zong Comparative transcriptome analysis of the newly discovered insect vector of the pine wood nematode in China, revealing putative genes related to host plant adaptation |
topic_facet |
Cerambycidae Monochamus saltuarius Host adaptation Transcriptional variation Pinus koraiensis Pinus tabuliformis Biotechnology TP248.13-248.65 Genetics QH426-470 |
description |
Abstract Background In many insect species, the larvae/nymphs are unable to disperse far from the oviposition site selected by adults. The Sakhalin pine sawyer Monochamus saltuarius (Gebler) is the newly discovered insect vector of the pine wood nematode (Bursaphelenchus xylophilus) in China. Adult M. saltuarius prefers to oviposit on the host plant Pinus koraiensis, rather than P. tabuliformis. However, the genetic basis of adaptation of the larvae of M. saltuarius with weaken dispersal ability to host environments selected by the adult is not well understood. Results In this study, the free amino and fatty acid composition and content of the host plants of M. saltuarius larvae, i.e., P. koraiensis and P. tabuliformis were investigated. Compared with P. koraiensis, P. tabuliformis had a substantially higher content of various free amino acids, while the opposite trend was detected for fatty acid content. The transcriptional profiles of larval populations feeding on P. koraiensis and P. tabuliformis were compared using PacBio Sequel II sequencing combined with Illumina sequencing. The results showed that genes relating to digestion, fatty acid synthesis, detoxification, oxidation-reduction, and stress response, as well as nutrients and energy sensing ability, were differentially expressed, possibly reflecting adaptive changes of M. saltuarius in response to different host diets. Additionally, genes coding for cuticle structure were differentially expressed, indicating that cuticle may be a potential target for plant defense. Differential regulation of genes related to the antibacterial and immune response were also observed, suggesting that larvae of M. saltuarius may have evolved adaptations to cope with bacterial challenges in their host environments. Conclusions The present study provides comprehensive transcriptome resource of M. saltuarius relating to host plant adaptation. Results from this study help to illustrate the fundamental relationship between transcriptional plasticity and adaptation mechanisms of ... |
format |
Article in Journal/Newspaper |
author |
Zehai Hou Fengming Shi Sixun Ge Jing Tao Lili Ren Hao Wu Shixiang Zong |
author_facet |
Zehai Hou Fengming Shi Sixun Ge Jing Tao Lili Ren Hao Wu Shixiang Zong |
author_sort |
Zehai Hou |
title |
Comparative transcriptome analysis of the newly discovered insect vector of the pine wood nematode in China, revealing putative genes related to host plant adaptation |
title_short |
Comparative transcriptome analysis of the newly discovered insect vector of the pine wood nematode in China, revealing putative genes related to host plant adaptation |
title_full |
Comparative transcriptome analysis of the newly discovered insect vector of the pine wood nematode in China, revealing putative genes related to host plant adaptation |
title_fullStr |
Comparative transcriptome analysis of the newly discovered insect vector of the pine wood nematode in China, revealing putative genes related to host plant adaptation |
title_full_unstemmed |
Comparative transcriptome analysis of the newly discovered insect vector of the pine wood nematode in China, revealing putative genes related to host plant adaptation |
title_sort |
comparative transcriptome analysis of the newly discovered insect vector of the pine wood nematode in china, revealing putative genes related to host plant adaptation |
publisher |
BMC |
publishDate |
2021 |
url |
https://doi.org/10.1186/s12864-021-07498-1 https://doaj.org/article/8dc19aff78164a4f90dbd56b1e8bd6e5 |
genre |
Sakhalin |
genre_facet |
Sakhalin |
op_source |
BMC Genomics, Vol 22, Iss 1, Pp 1-16 (2021) |
op_relation |
https://doi.org/10.1186/s12864-021-07498-1 https://doaj.org/toc/1471-2164 doi:10.1186/s12864-021-07498-1 1471-2164 https://doaj.org/article/8dc19aff78164a4f90dbd56b1e8bd6e5 |
op_doi |
https://doi.org/10.1186/s12864-021-07498-1 |
container_title |
BMC Genomics |
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22 |
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1 |
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1766181756467150848 |