Comparison of serum hepatitis B virus replication markers in patients with chronic hepatitis B: studies on HBeAg/Anti-HBe system, viral dna polymerase and HBV-DNA Comparação dos marcadores sorológicos da replicação do vírus da hepatite B: estudos sobre o sistema AgHBe/anti-HBe, DNA polimerase virai e HBV-DNA
The detection of HBV-DNA in serum by molecular hybridization is the most sensitive and specific marker of replication and infectivity of hepatitis B virus and currently is proposed as a routine diagnostic technique in the follow-up of HBV - related diseases. Comparing different techniques already de...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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Language: | English |
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Universidade de São Paulo (USP)
1989
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ftdoajarticles:oai:doaj.org/article:8da92c537e8e4226949ad73007fb376b 2024-09-09T19:28:36+00:00 Comparison of serum hepatitis B virus replication markers in patients with chronic hepatitis B: studies on HBeAg/Anti-HBe system, viral dna polymerase and HBV-DNA Comparação dos marcadores sorológicos da replicação do vírus da hepatite B: estudos sobre o sistema AgHBe/anti-HBe, DNA polimerase virai e HBV-DNA João Renato Rebello Pinho Luís Edmundo Pinto da Fonseca Yu Song Yuriko Miyamoto Flair José Carrilho Celso Francisco Hernandes Granato Luiz Caetano da Silva 1989-10-01T00:00:00Z https://doi.org/10.1590/S0036-46651989000500006 https://doaj.org/article/8da92c537e8e4226949ad73007fb376b EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651989000500006 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651989000500006 0036-4665 1678-9946 https://doaj.org/article/8da92c537e8e4226949ad73007fb376b Revista do Instituto de Medicina Tropical de São Paulo, Vol 31, Iss 5, Pp 328-335 (1989) Hepatitis B Molecular hybridization DNA polymerase HBV-DNA HBV replication markers Chronic hepatitis B Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1989 ftdoajarticles https://doi.org/10.1590/S0036-46651989000500006 2024-08-05T17:49:31Z The detection of HBV-DNA in serum by molecular hybridization is the most sensitive and specific marker of replication and infectivity of hepatitis B virus and currently is proposed as a routine diagnostic technique in the follow-up of HBV - related diseases. Comparing different techniques already described, we found that direct spotting of serum samples on nitrocellulose membranes under vacuum filtration, followed by denaturing and neutralizing washes is more practical, simple, sensible and reproducible. DNA polymerase assay using phosphonoformic acid as specific viral inhibitor has shown 86.8% of concordance with HBV-DNA detection, and so, it is an useful alternative in the follow-up of hepatitis B chronic patients. We found 19.2% HBeAg positive samples with no other markers of viral replication and no anti-HBe positive sample had detectable HBV-DNA. Discordance between the 2 systems have been extensively described, and we confirm this for the first time in our country. Molecular biological techniques are essential to determine the replication status of chronic hepatitis B patients. A detecção do genoma do HBV no soro por hibridização molecular é o mais sensível e específico marcador da replicação e infectividade do HBV, sendo sua utilização proposta como técnica rotineira no acompanhamento de doenças relacionadas a este vírus. Comparando diferentes técnicas descritas anteriormente, escolhemos a deposição direta das amostras séricas sobre a membrana de nitrocelulose sob filtração a vácuo, seguida de banhos desnaturantes e neutralisantes como mais prática e simples, com sensibilidade equivalente. O ensaio da DNA polimerase usando ácido fosfonofórmico como inibidor virai específico mostrou 86.8% de concordância com a detecção direta do DNA viral, sendo, portanto, uma alternativa viável no acompanhamento de pacientes com hepatite crônica B. Encontramos 19,2% das amostras AgHBe positivos sem outros marcadores de replicação viral. Por outro lado, nenhuma amostra anti-HBe positiva teve HBV-DNA detectável. ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Sob’ ENVELOPE(66.156,66.156,66.322,66.322) Revista do Instituto de Medicina Tropical de São Paulo 31 5 328 335 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Hepatitis B Molecular hybridization DNA polymerase HBV-DNA HBV replication markers Chronic hepatitis B Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Hepatitis B Molecular hybridization DNA polymerase HBV-DNA HBV replication markers Chronic hepatitis B Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 João Renato Rebello Pinho Luís Edmundo Pinto da Fonseca Yu Song Yuriko Miyamoto Flair José Carrilho Celso Francisco Hernandes Granato Luiz Caetano da Silva Comparison of serum hepatitis B virus replication markers in patients with chronic hepatitis B: studies on HBeAg/Anti-HBe system, viral dna polymerase and HBV-DNA Comparação dos marcadores sorológicos da replicação do vírus da hepatite B: estudos sobre o sistema AgHBe/anti-HBe, DNA polimerase virai e HBV-DNA |
topic_facet |
Hepatitis B Molecular hybridization DNA polymerase HBV-DNA HBV replication markers Chronic hepatitis B Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
The detection of HBV-DNA in serum by molecular hybridization is the most sensitive and specific marker of replication and infectivity of hepatitis B virus and currently is proposed as a routine diagnostic technique in the follow-up of HBV - related diseases. Comparing different techniques already described, we found that direct spotting of serum samples on nitrocellulose membranes under vacuum filtration, followed by denaturing and neutralizing washes is more practical, simple, sensible and reproducible. DNA polymerase assay using phosphonoformic acid as specific viral inhibitor has shown 86.8% of concordance with HBV-DNA detection, and so, it is an useful alternative in the follow-up of hepatitis B chronic patients. We found 19.2% HBeAg positive samples with no other markers of viral replication and no anti-HBe positive sample had detectable HBV-DNA. Discordance between the 2 systems have been extensively described, and we confirm this for the first time in our country. Molecular biological techniques are essential to determine the replication status of chronic hepatitis B patients. A detecção do genoma do HBV no soro por hibridização molecular é o mais sensível e específico marcador da replicação e infectividade do HBV, sendo sua utilização proposta como técnica rotineira no acompanhamento de doenças relacionadas a este vírus. Comparando diferentes técnicas descritas anteriormente, escolhemos a deposição direta das amostras séricas sobre a membrana de nitrocelulose sob filtração a vácuo, seguida de banhos desnaturantes e neutralisantes como mais prática e simples, com sensibilidade equivalente. O ensaio da DNA polimerase usando ácido fosfonofórmico como inibidor virai específico mostrou 86.8% de concordância com a detecção direta do DNA viral, sendo, portanto, uma alternativa viável no acompanhamento de pacientes com hepatite crônica B. Encontramos 19,2% das amostras AgHBe positivos sem outros marcadores de replicação viral. Por outro lado, nenhuma amostra anti-HBe positiva teve HBV-DNA detectável. ... |
format |
Article in Journal/Newspaper |
author |
João Renato Rebello Pinho Luís Edmundo Pinto da Fonseca Yu Song Yuriko Miyamoto Flair José Carrilho Celso Francisco Hernandes Granato Luiz Caetano da Silva |
author_facet |
João Renato Rebello Pinho Luís Edmundo Pinto da Fonseca Yu Song Yuriko Miyamoto Flair José Carrilho Celso Francisco Hernandes Granato Luiz Caetano da Silva |
author_sort |
João Renato Rebello Pinho |
title |
Comparison of serum hepatitis B virus replication markers in patients with chronic hepatitis B: studies on HBeAg/Anti-HBe system, viral dna polymerase and HBV-DNA Comparação dos marcadores sorológicos da replicação do vírus da hepatite B: estudos sobre o sistema AgHBe/anti-HBe, DNA polimerase virai e HBV-DNA |
title_short |
Comparison of serum hepatitis B virus replication markers in patients with chronic hepatitis B: studies on HBeAg/Anti-HBe system, viral dna polymerase and HBV-DNA Comparação dos marcadores sorológicos da replicação do vírus da hepatite B: estudos sobre o sistema AgHBe/anti-HBe, DNA polimerase virai e HBV-DNA |
title_full |
Comparison of serum hepatitis B virus replication markers in patients with chronic hepatitis B: studies on HBeAg/Anti-HBe system, viral dna polymerase and HBV-DNA Comparação dos marcadores sorológicos da replicação do vírus da hepatite B: estudos sobre o sistema AgHBe/anti-HBe, DNA polimerase virai e HBV-DNA |
title_fullStr |
Comparison of serum hepatitis B virus replication markers in patients with chronic hepatitis B: studies on HBeAg/Anti-HBe system, viral dna polymerase and HBV-DNA Comparação dos marcadores sorológicos da replicação do vírus da hepatite B: estudos sobre o sistema AgHBe/anti-HBe, DNA polimerase virai e HBV-DNA |
title_full_unstemmed |
Comparison of serum hepatitis B virus replication markers in patients with chronic hepatitis B: studies on HBeAg/Anti-HBe system, viral dna polymerase and HBV-DNA Comparação dos marcadores sorológicos da replicação do vírus da hepatite B: estudos sobre o sistema AgHBe/anti-HBe, DNA polimerase virai e HBV-DNA |
title_sort |
comparison of serum hepatitis b virus replication markers in patients with chronic hepatitis b: studies on hbeag/anti-hbe system, viral dna polymerase and hbv-dna comparação dos marcadores sorológicos da replicação do vírus da hepatite b: estudos sobre o sistema aghbe/anti-hbe, dna polimerase virai e hbv-dna |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1989 |
url |
https://doi.org/10.1590/S0036-46651989000500006 https://doaj.org/article/8da92c537e8e4226949ad73007fb376b |
long_lat |
ENVELOPE(66.156,66.156,66.322,66.322) |
geographic |
Arctic Sob’ |
geographic_facet |
Arctic Sob’ |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 31, Iss 5, Pp 328-335 (1989) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651989000500006 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651989000500006 0036-4665 1678-9946 https://doaj.org/article/8da92c537e8e4226949ad73007fb376b |
op_doi |
https://doi.org/10.1590/S0036-46651989000500006 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
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31 |
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328 |
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