Epidemiological, molecular characterization and risk factors of human brucellosis in Iran
Objective: To determine epidemiological, molecular characterization, and potential risk factors of human brucellosis. Methods: This descriptive study was carried out in the clinical setting in Iran between 2017 and 2018. A total of 297 participants enrolled in the study. The sample size was calculat...
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ftdoajarticles:oai:doaj.org/article:8da3122fa9b0481fa54c7e6a9639db6c 2023-05-15T15:09:21+02:00 Epidemiological, molecular characterization and risk factors of human brucellosis in Iran Afshar Etemadi Rezvan Moniri Mahmood Saffari Hossein Akbari Saeed Alamian Ali Mohammad Behrozikhah 2020-01-01T00:00:00Z https://doi.org/10.4103/1995-7645.280224 https://doaj.org/article/8da3122fa9b0481fa54c7e6a9639db6c EN eng Wolters Kluwer Medknow Publications http://www.apjtm.org/article.asp?issn=1995-7645;year=2020;volume=13;issue=4;spage=169;epage=175;aulast=Etemadi https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.280224 https://doaj.org/article/8da3122fa9b0481fa54c7e6a9639db6c Asian Pacific Journal of Tropical Medicine, Vol 13, Iss 4, Pp 169-175 (2020) brucellosis epidemiology molecular detection risk factors Arctic medicine. Tropical medicine RC955-962 article 2020 ftdoajarticles https://doi.org/10.4103/1995-7645.280224 2022-12-31T11:09:43Z Objective: To determine epidemiological, molecular characterization, and potential risk factors of human brucellosis. Methods: This descriptive study was carried out in the clinical setting in Iran between 2017 and 2018. A total of 297 participants enrolled in the study. The sample size was calculated based on the occurrence rate of brucellosis in different areas. Patients were assessed using serological tests and conventional culture methods. Phage and multiplex PCR methods typed all of Brucella isolates. Potential risk factors of disease were determined. Results: A total of 141 of 297 (47.5%) Brucella strains were isolated and all of them were detected as Brucella melitensis biovar 1. Based on serologic titers, high culture positivity was recorded at 1/640 titer (P< 0.006). The risk factors for brucellosis were patients older than 40 years (OR=2.23, 95%CI: 1.4-3.55, P=0.001), animal keeper (OR=7, 95%CI: 1.51-32.41, P=0.005), housewife (OR=8.76, 95%CI: 1.85-41.37, P=0.002), farmer (OR=6.42, 95%CI: 1.21-33.97, P=0.019), and contact with animal (OR=1.31, 95%CI: 0.60-2.85, P=0.005). Conclusions: To the best of our knowledge, this is the first comprehensive report from Iran presenting the detection of Brucella species by the multiplex PCR. Brucella melitensis biovar 1 is still the dominant causative agent in Iran. The consumption of unpasteurized dairy products, living in rural areas, and animal contact were risk factors of brucellosis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Medicine 13 4 169 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
brucellosis epidemiology molecular detection risk factors Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
brucellosis epidemiology molecular detection risk factors Arctic medicine. Tropical medicine RC955-962 Afshar Etemadi Rezvan Moniri Mahmood Saffari Hossein Akbari Saeed Alamian Ali Mohammad Behrozikhah Epidemiological, molecular characterization and risk factors of human brucellosis in Iran |
topic_facet |
brucellosis epidemiology molecular detection risk factors Arctic medicine. Tropical medicine RC955-962 |
description |
Objective: To determine epidemiological, molecular characterization, and potential risk factors of human brucellosis. Methods: This descriptive study was carried out in the clinical setting in Iran between 2017 and 2018. A total of 297 participants enrolled in the study. The sample size was calculated based on the occurrence rate of brucellosis in different areas. Patients were assessed using serological tests and conventional culture methods. Phage and multiplex PCR methods typed all of Brucella isolates. Potential risk factors of disease were determined. Results: A total of 141 of 297 (47.5%) Brucella strains were isolated and all of them were detected as Brucella melitensis biovar 1. Based on serologic titers, high culture positivity was recorded at 1/640 titer (P< 0.006). The risk factors for brucellosis were patients older than 40 years (OR=2.23, 95%CI: 1.4-3.55, P=0.001), animal keeper (OR=7, 95%CI: 1.51-32.41, P=0.005), housewife (OR=8.76, 95%CI: 1.85-41.37, P=0.002), farmer (OR=6.42, 95%CI: 1.21-33.97, P=0.019), and contact with animal (OR=1.31, 95%CI: 0.60-2.85, P=0.005). Conclusions: To the best of our knowledge, this is the first comprehensive report from Iran presenting the detection of Brucella species by the multiplex PCR. Brucella melitensis biovar 1 is still the dominant causative agent in Iran. The consumption of unpasteurized dairy products, living in rural areas, and animal contact were risk factors of brucellosis. |
format |
Article in Journal/Newspaper |
author |
Afshar Etemadi Rezvan Moniri Mahmood Saffari Hossein Akbari Saeed Alamian Ali Mohammad Behrozikhah |
author_facet |
Afshar Etemadi Rezvan Moniri Mahmood Saffari Hossein Akbari Saeed Alamian Ali Mohammad Behrozikhah |
author_sort |
Afshar Etemadi |
title |
Epidemiological, molecular characterization and risk factors of human brucellosis in Iran |
title_short |
Epidemiological, molecular characterization and risk factors of human brucellosis in Iran |
title_full |
Epidemiological, molecular characterization and risk factors of human brucellosis in Iran |
title_fullStr |
Epidemiological, molecular characterization and risk factors of human brucellosis in Iran |
title_full_unstemmed |
Epidemiological, molecular characterization and risk factors of human brucellosis in Iran |
title_sort |
epidemiological, molecular characterization and risk factors of human brucellosis in iran |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2020 |
url |
https://doi.org/10.4103/1995-7645.280224 https://doaj.org/article/8da3122fa9b0481fa54c7e6a9639db6c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Medicine, Vol 13, Iss 4, Pp 169-175 (2020) |
op_relation |
http://www.apjtm.org/article.asp?issn=1995-7645;year=2020;volume=13;issue=4;spage=169;epage=175;aulast=Etemadi https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.280224 https://doaj.org/article/8da3122fa9b0481fa54c7e6a9639db6c |
op_doi |
https://doi.org/10.4103/1995-7645.280224 |
container_title |
Asian Pacific Journal of Tropical Medicine |
container_volume |
13 |
container_issue |
4 |
container_start_page |
169 |
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1766340563673546752 |