Mosquito population structure, pathogen surveillance and insecticide resistance monitoring in urban regions of Crete, Greece.
Background In Greece vector borne diseases (VBD) and foremost West Nile virus (WNV) pose an important threat to public health and the tourist industry, the primary sector of contribution to the national economy. The island of Crete, is one of Greece's major tourist destinations receiving annual...
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ftdoajarticles:oai:doaj.org/article:8d9b1df10ffe4e3f908649b0dc02b68b 2023-05-15T15:15:16+02:00 Mosquito population structure, pathogen surveillance and insecticide resistance monitoring in urban regions of Crete, Greece. Emmanouil A Fotakis Konstantinos Mavridis Anastasia Kampouraki Sofia Balaska Filianna Tanti George Vlachos Sandra Gewehr Spiros Mourelatos Antonios Papadakis Maria Kavalou Dimitrios Nikolakakis Maria Moisaki Nikolaos Kampanis Manolis Loumpounis John Vontas 2022-02-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010186 https://doaj.org/article/8d9b1df10ffe4e3f908649b0dc02b68b EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010186 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010186 https://doaj.org/article/8d9b1df10ffe4e3f908649b0dc02b68b PLoS Neglected Tropical Diseases, Vol 16, Iss 2, p e0010186 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010186 2022-12-31T15:49:17Z Background In Greece vector borne diseases (VBD) and foremost West Nile virus (WNV) pose an important threat to public health and the tourist industry, the primary sector of contribution to the national economy. The island of Crete, is one of Greece's major tourist destinations receiving annually over 5 million tourists making regional VBD control both a public health and economic priority. Methodology Under the auspices of the Region of Crete, a systematic integrative surveillance network targeting mosquitoes and associated pathogens was established in Crete for the years 2018-2020. Using conventional and molecular diagnostic tools we investigated the mosquito species composition and population dynamics, pathogen infection occurrences in vector populations and in sentinel chickens, and the insecticide resistance status of the major vector species. Principal findings Important disease vectors were recorded across the island including Culex pipiens, Aedes albopictus, and Anopheles superpictus. Over 75% of the sampled specimens were collected in the western prefectures potentially attributed to the local precipitation patterns, with Cx. pipiens being the most dominant species. Although no pathogens (flaviviruses) were detected in the analysed mosquito specimens, chicken blood serum analyses recorded a 1.7% WNV antibody detection rate in the 2018 samples. Notably detection of the first WNV positive chicken preceded human WNV occurrence in the same region by approximately two weeks. The chitin synthase mutation I1043F (associated with high diflubenzuron resistance) was recorded at an 8% allelic frequency in Lasithi prefecture Cx. pipiens mosquitoes (sampled in 2020) for the first time in Greece. Markedly, Cx. pipiens populations in all four prefectures were found harboring the kdr mutations L1014F/C/S (associated with pyrethroid resistance) at a close to fixation rate, with mutation L1014C being the most commonly found allele (≥74% representation). Voltage gated sodium channel analyses in Ae. albopictus revealed the ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 2 e0010186 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Emmanouil A Fotakis Konstantinos Mavridis Anastasia Kampouraki Sofia Balaska Filianna Tanti George Vlachos Sandra Gewehr Spiros Mourelatos Antonios Papadakis Maria Kavalou Dimitrios Nikolakakis Maria Moisaki Nikolaos Kampanis Manolis Loumpounis John Vontas Mosquito population structure, pathogen surveillance and insecticide resistance monitoring in urban regions of Crete, Greece. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background In Greece vector borne diseases (VBD) and foremost West Nile virus (WNV) pose an important threat to public health and the tourist industry, the primary sector of contribution to the national economy. The island of Crete, is one of Greece's major tourist destinations receiving annually over 5 million tourists making regional VBD control both a public health and economic priority. Methodology Under the auspices of the Region of Crete, a systematic integrative surveillance network targeting mosquitoes and associated pathogens was established in Crete for the years 2018-2020. Using conventional and molecular diagnostic tools we investigated the mosquito species composition and population dynamics, pathogen infection occurrences in vector populations and in sentinel chickens, and the insecticide resistance status of the major vector species. Principal findings Important disease vectors were recorded across the island including Culex pipiens, Aedes albopictus, and Anopheles superpictus. Over 75% of the sampled specimens were collected in the western prefectures potentially attributed to the local precipitation patterns, with Cx. pipiens being the most dominant species. Although no pathogens (flaviviruses) were detected in the analysed mosquito specimens, chicken blood serum analyses recorded a 1.7% WNV antibody detection rate in the 2018 samples. Notably detection of the first WNV positive chicken preceded human WNV occurrence in the same region by approximately two weeks. The chitin synthase mutation I1043F (associated with high diflubenzuron resistance) was recorded at an 8% allelic frequency in Lasithi prefecture Cx. pipiens mosquitoes (sampled in 2020) for the first time in Greece. Markedly, Cx. pipiens populations in all four prefectures were found harboring the kdr mutations L1014F/C/S (associated with pyrethroid resistance) at a close to fixation rate, with mutation L1014C being the most commonly found allele (≥74% representation). Voltage gated sodium channel analyses in Ae. albopictus revealed the ... |
format |
Article in Journal/Newspaper |
author |
Emmanouil A Fotakis Konstantinos Mavridis Anastasia Kampouraki Sofia Balaska Filianna Tanti George Vlachos Sandra Gewehr Spiros Mourelatos Antonios Papadakis Maria Kavalou Dimitrios Nikolakakis Maria Moisaki Nikolaos Kampanis Manolis Loumpounis John Vontas |
author_facet |
Emmanouil A Fotakis Konstantinos Mavridis Anastasia Kampouraki Sofia Balaska Filianna Tanti George Vlachos Sandra Gewehr Spiros Mourelatos Antonios Papadakis Maria Kavalou Dimitrios Nikolakakis Maria Moisaki Nikolaos Kampanis Manolis Loumpounis John Vontas |
author_sort |
Emmanouil A Fotakis |
title |
Mosquito population structure, pathogen surveillance and insecticide resistance monitoring in urban regions of Crete, Greece. |
title_short |
Mosquito population structure, pathogen surveillance and insecticide resistance monitoring in urban regions of Crete, Greece. |
title_full |
Mosquito population structure, pathogen surveillance and insecticide resistance monitoring in urban regions of Crete, Greece. |
title_fullStr |
Mosquito population structure, pathogen surveillance and insecticide resistance monitoring in urban regions of Crete, Greece. |
title_full_unstemmed |
Mosquito population structure, pathogen surveillance and insecticide resistance monitoring in urban regions of Crete, Greece. |
title_sort |
mosquito population structure, pathogen surveillance and insecticide resistance monitoring in urban regions of crete, greece. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010186 https://doaj.org/article/8d9b1df10ffe4e3f908649b0dc02b68b |
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Arctic |
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Arctic |
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Arctic |
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Arctic |
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PLoS Neglected Tropical Diseases, Vol 16, Iss 2, p e0010186 (2022) |
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https://doi.org/10.1371/journal.pntd.0010186 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010186 https://doaj.org/article/8d9b1df10ffe4e3f908649b0dc02b68b |
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https://doi.org/10.1371/journal.pntd.0010186 |
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PLOS Neglected Tropical Diseases |
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e0010186 |
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