Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae)
The autumnal moth (Epirrita autumnata) is a cyclically outbreaking forest Lepidoptera with circumpolar distribution and substantial impact on Northern ecosystems. We have isolated 21 microsatellites from the species to facilitate population genetic studies of population cycles, outbreaks, and crashe...
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ftdoajarticles:oai:doaj.org/article:8d949cbd65d0463a8fa9ea2b1c5023f4 2023-05-15T17:43:30+02:00 Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae) Siv Grethe Aarnes Ida Fløystad Julia Schregel Ole Petter Laksforsmo Vindstad Jane Uhd Jepsen Hans Geir Eiken Rolf A. Ims Snorre B. Hagen 2015-09-01T00:00:00Z https://doi.org/10.3390/ijms160922541 https://doaj.org/article/8d949cbd65d0463a8fa9ea2b1c5023f4 EN eng MDPI AG http://www.mdpi.com/1422-0067/16/9/22541 https://doaj.org/toc/1422-0067 1422-0067 doi:10.3390/ijms160922541 https://doaj.org/article/8d949cbd65d0463a8fa9ea2b1c5023f4 International Journal of Molecular Sciences, Vol 16, Iss 9, Pp 22541-22554 (2015) tri- and tetranucleotide microsatellites multiplex PCR Lepidoptera population genetics Biology (General) QH301-705.5 Chemistry QD1-999 article 2015 ftdoajarticles https://doi.org/10.3390/ijms160922541 2022-12-31T14:41:20Z The autumnal moth (Epirrita autumnata) is a cyclically outbreaking forest Lepidoptera with circumpolar distribution and substantial impact on Northern ecosystems. We have isolated 21 microsatellites from the species to facilitate population genetic studies of population cycles, outbreaks, and crashes. First, PCR primers and PCR conditions were developed to amplify 19 trinucleotide loci and two tetranucleotide loci in six multiplex PCR approaches and then analyzed for species specificity, sensitivity and precision. Twelve of the loci showed simple tandem repeat array structures while nine loci showed imperfect repeat structures, and repeat numbers varied in our material between six and 15. The application in population genetics for all the 21 microsatellites were further validated in 48 autumnal moths sampled from Northern Norway, and allelic variation was detected in 19 loci. The detected numbers of alleles per locus ranged from two to 13, and the observed and expected heterozygosities varied from 0.04 to 0.69 and 0.04 to 0.79, respectively. Evidence for linkage disequilibrium was found for six loci as well as indication of one null allele. We find that these novel microsatellites and their multiplex-PCR assays are suitable for further research on fine- and large-scale population-genetic studies of Epirrita autumnata. Article in Journal/Newspaper Northern Norway Directory of Open Access Journals: DOAJ Articles Norway International Journal of Molecular Sciences 16 9 22541 22554 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
tri- and tetranucleotide microsatellites multiplex PCR Lepidoptera population genetics Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
tri- and tetranucleotide microsatellites multiplex PCR Lepidoptera population genetics Biology (General) QH301-705.5 Chemistry QD1-999 Siv Grethe Aarnes Ida Fløystad Julia Schregel Ole Petter Laksforsmo Vindstad Jane Uhd Jepsen Hans Geir Eiken Rolf A. Ims Snorre B. Hagen Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae) |
topic_facet |
tri- and tetranucleotide microsatellites multiplex PCR Lepidoptera population genetics Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
The autumnal moth (Epirrita autumnata) is a cyclically outbreaking forest Lepidoptera with circumpolar distribution and substantial impact on Northern ecosystems. We have isolated 21 microsatellites from the species to facilitate population genetic studies of population cycles, outbreaks, and crashes. First, PCR primers and PCR conditions were developed to amplify 19 trinucleotide loci and two tetranucleotide loci in six multiplex PCR approaches and then analyzed for species specificity, sensitivity and precision. Twelve of the loci showed simple tandem repeat array structures while nine loci showed imperfect repeat structures, and repeat numbers varied in our material between six and 15. The application in population genetics for all the 21 microsatellites were further validated in 48 autumnal moths sampled from Northern Norway, and allelic variation was detected in 19 loci. The detected numbers of alleles per locus ranged from two to 13, and the observed and expected heterozygosities varied from 0.04 to 0.69 and 0.04 to 0.79, respectively. Evidence for linkage disequilibrium was found for six loci as well as indication of one null allele. We find that these novel microsatellites and their multiplex-PCR assays are suitable for further research on fine- and large-scale population-genetic studies of Epirrita autumnata. |
format |
Article in Journal/Newspaper |
author |
Siv Grethe Aarnes Ida Fløystad Julia Schregel Ole Petter Laksforsmo Vindstad Jane Uhd Jepsen Hans Geir Eiken Rolf A. Ims Snorre B. Hagen |
author_facet |
Siv Grethe Aarnes Ida Fløystad Julia Schregel Ole Petter Laksforsmo Vindstad Jane Uhd Jepsen Hans Geir Eiken Rolf A. Ims Snorre B. Hagen |
author_sort |
Siv Grethe Aarnes |
title |
Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae) |
title_short |
Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae) |
title_full |
Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae) |
title_fullStr |
Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae) |
title_full_unstemmed |
Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae) |
title_sort |
identification and evaluation of 21 novel microsatellite markers from the autumnal moth (epirrita autumnata) (lepidoptera: geometridae) |
publisher |
MDPI AG |
publishDate |
2015 |
url |
https://doi.org/10.3390/ijms160922541 https://doaj.org/article/8d949cbd65d0463a8fa9ea2b1c5023f4 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Northern Norway |
genre_facet |
Northern Norway |
op_source |
International Journal of Molecular Sciences, Vol 16, Iss 9, Pp 22541-22554 (2015) |
op_relation |
http://www.mdpi.com/1422-0067/16/9/22541 https://doaj.org/toc/1422-0067 1422-0067 doi:10.3390/ijms160922541 https://doaj.org/article/8d949cbd65d0463a8fa9ea2b1c5023f4 |
op_doi |
https://doi.org/10.3390/ijms160922541 |
container_title |
International Journal of Molecular Sciences |
container_volume |
16 |
container_issue |
9 |
container_start_page |
22541 |
op_container_end_page |
22554 |
_version_ |
1766145602443280384 |