Usefulness of real-time PCR for urogenital schistosomiasis diagnosis in preschool children in a high-prevalence area in Angola.
Background Urogenital schistosomiasis caused by Schistosoma haematobium is highly endemic in the municipality of Cubal in Angola. Currently, diagnosis is based on the observation of S. haematobium eggs in urine samples by microscopy but this method has low sensitivity. Few studies have been performe...
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ftdoajarticles:oai:doaj.org/article:8d91941aab8d430e9fe2a836446a155e 2024-09-09T19:28:15+00:00 Usefulness of real-time PCR for urogenital schistosomiasis diagnosis in preschool children in a high-prevalence area in Angola. Alejandro Mediavilla Aroa Silgado Raquel Sánchez-Marqués Cristina Bocanegra Arlette Nindia Fernando Salvador Zeferino Pintar Patricia Martínez-Vallejo Carles Rubio Maturana Lidia Goterris Joan Martínez-Campreciós Sandra Aixut Inés Oliveira-Souto María Luisa Aznar-Ruiz-de-Alegría María Espiau Israel Molina Elena Sulleiro 2024-08-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0012384 https://doaj.org/article/8d91941aab8d430e9fe2a836446a155e EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0012384 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0012384 https://doaj.org/article/8d91941aab8d430e9fe2a836446a155e PLoS Neglected Tropical Diseases, Vol 18, Iss 8, p e0012384 (2024) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2024 ftdoajarticles https://doi.org/10.1371/journal.pntd.0012384 2024-08-26T15:21:14Z Background Urogenital schistosomiasis caused by Schistosoma haematobium is highly endemic in the municipality of Cubal in Angola. Currently, diagnosis is based on the observation of S. haematobium eggs in urine samples by microscopy but this method has low sensitivity. Few studies have been performed using molecular techniques in high-prevalence areas for the detection of S. haematobium. The objective of this study is to evaluate the usefulness of real-time PCR as a diagnostic technique for urogenital schistosomiasis among preschool-age children and its correlation with morbidity data. Methods A cross-sectional study was conducted in Cubal, Angola, involving 97 urine samples from preschool-age children analyzed by the dipstick test, microscopic examination of filtered urine, and real-time PCR. The diagnosis of urogenital schistosomiasis was based on microscopy and/or real-time PCR results. Clinical and ultrasonography evaluation was performed to rule out complications of schistosomiasis. Results We detected a total of 64.95% of samples positive by real-time PCR and 37.11% by microscopy. The sensitivity of parasitological diagnosis of urogenital schistosomiasis by real-time PCR and microscopy was 95.45% and 54.55%, respectively, and the sensitivity of real-time PCR compared with microscopy was 91.67%. A positive real-time PCR result was significantly related to older age (mean = 3.22 years), detection of eggs by microscopy, and abnormal urine dipstick results (18.56% with proteinuria, 31.96% with leukocyturia, and 31.96% with microhematuria) (p-value<0.05). Ultrasound analysis showed that 23.94% of children had urinary tract abnormalities, and it was significantly related to the real-time PCR diagnosis (p-value<0.05). Conclusions Real-time PCR is a more sensitive technique than microscopy for urinary schistosomiasis diagnosis in preschool-age children in Cubal. This increase in sensitivity would allow earlier diagnosis and treatment, thus reducing the morbidity associated with schistosomiasis in its early ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 18 8 e0012384 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Alejandro Mediavilla Aroa Silgado Raquel Sánchez-Marqués Cristina Bocanegra Arlette Nindia Fernando Salvador Zeferino Pintar Patricia Martínez-Vallejo Carles Rubio Maturana Lidia Goterris Joan Martínez-Campreciós Sandra Aixut Inés Oliveira-Souto María Luisa Aznar-Ruiz-de-Alegría María Espiau Israel Molina Elena Sulleiro Usefulness of real-time PCR for urogenital schistosomiasis diagnosis in preschool children in a high-prevalence area in Angola. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Urogenital schistosomiasis caused by Schistosoma haematobium is highly endemic in the municipality of Cubal in Angola. Currently, diagnosis is based on the observation of S. haematobium eggs in urine samples by microscopy but this method has low sensitivity. Few studies have been performed using molecular techniques in high-prevalence areas for the detection of S. haematobium. The objective of this study is to evaluate the usefulness of real-time PCR as a diagnostic technique for urogenital schistosomiasis among preschool-age children and its correlation with morbidity data. Methods A cross-sectional study was conducted in Cubal, Angola, involving 97 urine samples from preschool-age children analyzed by the dipstick test, microscopic examination of filtered urine, and real-time PCR. The diagnosis of urogenital schistosomiasis was based on microscopy and/or real-time PCR results. Clinical and ultrasonography evaluation was performed to rule out complications of schistosomiasis. Results We detected a total of 64.95% of samples positive by real-time PCR and 37.11% by microscopy. The sensitivity of parasitological diagnosis of urogenital schistosomiasis by real-time PCR and microscopy was 95.45% and 54.55%, respectively, and the sensitivity of real-time PCR compared with microscopy was 91.67%. A positive real-time PCR result was significantly related to older age (mean = 3.22 years), detection of eggs by microscopy, and abnormal urine dipstick results (18.56% with proteinuria, 31.96% with leukocyturia, and 31.96% with microhematuria) (p-value<0.05). Ultrasound analysis showed that 23.94% of children had urinary tract abnormalities, and it was significantly related to the real-time PCR diagnosis (p-value<0.05). Conclusions Real-time PCR is a more sensitive technique than microscopy for urinary schistosomiasis diagnosis in preschool-age children in Cubal. This increase in sensitivity would allow earlier diagnosis and treatment, thus reducing the morbidity associated with schistosomiasis in its early ... |
format |
Article in Journal/Newspaper |
author |
Alejandro Mediavilla Aroa Silgado Raquel Sánchez-Marqués Cristina Bocanegra Arlette Nindia Fernando Salvador Zeferino Pintar Patricia Martínez-Vallejo Carles Rubio Maturana Lidia Goterris Joan Martínez-Campreciós Sandra Aixut Inés Oliveira-Souto María Luisa Aznar-Ruiz-de-Alegría María Espiau Israel Molina Elena Sulleiro |
author_facet |
Alejandro Mediavilla Aroa Silgado Raquel Sánchez-Marqués Cristina Bocanegra Arlette Nindia Fernando Salvador Zeferino Pintar Patricia Martínez-Vallejo Carles Rubio Maturana Lidia Goterris Joan Martínez-Campreciós Sandra Aixut Inés Oliveira-Souto María Luisa Aznar-Ruiz-de-Alegría María Espiau Israel Molina Elena Sulleiro |
author_sort |
Alejandro Mediavilla |
title |
Usefulness of real-time PCR for urogenital schistosomiasis diagnosis in preschool children in a high-prevalence area in Angola. |
title_short |
Usefulness of real-time PCR for urogenital schistosomiasis diagnosis in preschool children in a high-prevalence area in Angola. |
title_full |
Usefulness of real-time PCR for urogenital schistosomiasis diagnosis in preschool children in a high-prevalence area in Angola. |
title_fullStr |
Usefulness of real-time PCR for urogenital schistosomiasis diagnosis in preschool children in a high-prevalence area in Angola. |
title_full_unstemmed |
Usefulness of real-time PCR for urogenital schistosomiasis diagnosis in preschool children in a high-prevalence area in Angola. |
title_sort |
usefulness of real-time pcr for urogenital schistosomiasis diagnosis in preschool children in a high-prevalence area in angola. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2024 |
url |
https://doi.org/10.1371/journal.pntd.0012384 https://doaj.org/article/8d91941aab8d430e9fe2a836446a155e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 18, Iss 8, p e0012384 (2024) |
op_relation |
https://doi.org/10.1371/journal.pntd.0012384 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0012384 https://doaj.org/article/8d91941aab8d430e9fe2a836446a155e |
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https://doi.org/10.1371/journal.pntd.0012384 |
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PLOS Neglected Tropical Diseases |
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