Temperature and injection water source influence microbial community structure in four Alaskan North Slope hydrocarbon reservoirs.
A fundamental knowledge of microbial community structure in petroleum reservoirs can improve predictive modeling of these environments. We used hydrocarbon profiles, stable isotopes, and high-density DNA microarray analysis to characterize microbial communities in produced water from four Alaska Nor...
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ftdoajarticles:oai:doaj.org/article:8d5acb19ce6d425ab32d277b7c024c68 2023-05-15T13:09:10+02:00 Temperature and injection water source influence microbial community structure in four Alaskan North Slope hydrocarbon reservoirs. Yvette Marisa Piceno Francine C Reid Lauren M Tom Mark E Conrad Markus eBill Christopher George Hubbard Bruce William Fouke Craig J Graff Jiabin eHan William T Stringfellow Jeremy S Hanlon Ping eHu Terry C Hazen Gary L Andersen 2014-08-01T00:00:00Z https://doi.org/10.3389/fmicb.2014.00409 https://doaj.org/article/8d5acb19ce6d425ab32d277b7c024c68 EN eng Frontiers Media S.A. http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00409/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2014.00409 https://doaj.org/article/8d5acb19ce6d425ab32d277b7c024c68 Frontiers in Microbiology, Vol 5 (2014) DNA microarray microbial communities Stable isotopes Souring Petroleum reservoirs Microbiology QR1-502 article 2014 ftdoajarticles https://doi.org/10.3389/fmicb.2014.00409 2022-12-31T14:58:04Z A fundamental knowledge of microbial community structure in petroleum reservoirs can improve predictive modeling of these environments. We used hydrocarbon profiles, stable isotopes, and high-density DNA microarray analysis to characterize microbial communities in produced water from four Alaska North Slope hydrocarbon reservoirs. Produced fluids from Schrader Bluff (24-27°C), Kuparuk (47-70°C), Sag River (80°C), and Ivishak (80-83°C) reservoirs were collected, with paired soured/non-soured wells sampled from Kuparuk and Ivishak. Chemical and stable isotope data suggested Schrader Bluff had substantial biogenic methane, whereas methane was mostly thermogenic in deeper reservoirs. Acetoclastic methanogens (Methanosaeta) were most prominent in Schrader Bluff samples, and the combined δD and δ13C values of methane also indicated acetoclastic methanogenesis could be a primary route for biogenic methane. Conversely, hydrogenotrophic methanogens (e.g., Methanobacteriaceae) and sulfide-producing Archaeoglobus and Thermococcus were more prominent in Kuparuk samples. Sulfide-producing microbes were detected in all reservoirs, uncoupled from souring status (e.g., the non-soured Kuparuk samples had higher relative abundances of many sulfate-reducers compared to the soured sample, suggesting sulfate-reducers may be living fermentatively/syntrophically when sulfate is limited). Sulfate abundance via long-term seawater injection resulted in greater relative abundances of Desulfonauticus, Desulfomicrobium, and Desulfuromonas in the soured Ivishak well compared to the non-soured well. In the non-soured Ivishak sample, several taxa affiliated with Thermoanaerobacter and Halomonas predominated. Archaea were not detected in the deepest reservoirs. Functional group taxa differed in relative abundance among reservoirs, likely reflecting differing thermal and/or geochemical influences. Article in Journal/Newspaper Alaska North Slope north slope Alaska Directory of Open Access Journals: DOAJ Articles Frontiers in Microbiology 5 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
DNA microarray microbial communities Stable isotopes Souring Petroleum reservoirs Microbiology QR1-502 |
spellingShingle |
DNA microarray microbial communities Stable isotopes Souring Petroleum reservoirs Microbiology QR1-502 Yvette Marisa Piceno Francine C Reid Lauren M Tom Mark E Conrad Markus eBill Christopher George Hubbard Bruce William Fouke Craig J Graff Jiabin eHan William T Stringfellow Jeremy S Hanlon Ping eHu Terry C Hazen Gary L Andersen Temperature and injection water source influence microbial community structure in four Alaskan North Slope hydrocarbon reservoirs. |
topic_facet |
DNA microarray microbial communities Stable isotopes Souring Petroleum reservoirs Microbiology QR1-502 |
description |
A fundamental knowledge of microbial community structure in petroleum reservoirs can improve predictive modeling of these environments. We used hydrocarbon profiles, stable isotopes, and high-density DNA microarray analysis to characterize microbial communities in produced water from four Alaska North Slope hydrocarbon reservoirs. Produced fluids from Schrader Bluff (24-27°C), Kuparuk (47-70°C), Sag River (80°C), and Ivishak (80-83°C) reservoirs were collected, with paired soured/non-soured wells sampled from Kuparuk and Ivishak. Chemical and stable isotope data suggested Schrader Bluff had substantial biogenic methane, whereas methane was mostly thermogenic in deeper reservoirs. Acetoclastic methanogens (Methanosaeta) were most prominent in Schrader Bluff samples, and the combined δD and δ13C values of methane also indicated acetoclastic methanogenesis could be a primary route for biogenic methane. Conversely, hydrogenotrophic methanogens (e.g., Methanobacteriaceae) and sulfide-producing Archaeoglobus and Thermococcus were more prominent in Kuparuk samples. Sulfide-producing microbes were detected in all reservoirs, uncoupled from souring status (e.g., the non-soured Kuparuk samples had higher relative abundances of many sulfate-reducers compared to the soured sample, suggesting sulfate-reducers may be living fermentatively/syntrophically when sulfate is limited). Sulfate abundance via long-term seawater injection resulted in greater relative abundances of Desulfonauticus, Desulfomicrobium, and Desulfuromonas in the soured Ivishak well compared to the non-soured well. In the non-soured Ivishak sample, several taxa affiliated with Thermoanaerobacter and Halomonas predominated. Archaea were not detected in the deepest reservoirs. Functional group taxa differed in relative abundance among reservoirs, likely reflecting differing thermal and/or geochemical influences. |
format |
Article in Journal/Newspaper |
author |
Yvette Marisa Piceno Francine C Reid Lauren M Tom Mark E Conrad Markus eBill Christopher George Hubbard Bruce William Fouke Craig J Graff Jiabin eHan William T Stringfellow Jeremy S Hanlon Ping eHu Terry C Hazen Gary L Andersen |
author_facet |
Yvette Marisa Piceno Francine C Reid Lauren M Tom Mark E Conrad Markus eBill Christopher George Hubbard Bruce William Fouke Craig J Graff Jiabin eHan William T Stringfellow Jeremy S Hanlon Ping eHu Terry C Hazen Gary L Andersen |
author_sort |
Yvette Marisa Piceno |
title |
Temperature and injection water source influence microbial community structure in four Alaskan North Slope hydrocarbon reservoirs. |
title_short |
Temperature and injection water source influence microbial community structure in four Alaskan North Slope hydrocarbon reservoirs. |
title_full |
Temperature and injection water source influence microbial community structure in four Alaskan North Slope hydrocarbon reservoirs. |
title_fullStr |
Temperature and injection water source influence microbial community structure in four Alaskan North Slope hydrocarbon reservoirs. |
title_full_unstemmed |
Temperature and injection water source influence microbial community structure in four Alaskan North Slope hydrocarbon reservoirs. |
title_sort |
temperature and injection water source influence microbial community structure in four alaskan north slope hydrocarbon reservoirs. |
publisher |
Frontiers Media S.A. |
publishDate |
2014 |
url |
https://doi.org/10.3389/fmicb.2014.00409 https://doaj.org/article/8d5acb19ce6d425ab32d277b7c024c68 |
genre |
Alaska North Slope north slope Alaska |
genre_facet |
Alaska North Slope north slope Alaska |
op_source |
Frontiers in Microbiology, Vol 5 (2014) |
op_relation |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00409/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2014.00409 https://doaj.org/article/8d5acb19ce6d425ab32d277b7c024c68 |
op_doi |
https://doi.org/10.3389/fmicb.2014.00409 |
container_title |
Frontiers in Microbiology |
container_volume |
5 |
_version_ |
1766165247851233280 |