Nuclear and kinetoplast DNA analyses reveal genetically complex Leishmania strains with hybrid and mito-nuclear discordance in Peru.
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the mannose phosphate isomerase (mpi) gene was applied to 134 skin samples collected from patients with cutaneous leishmaniasis (CL) in Peru for identification of the infecting parasite at the species level, an...
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ftdoajarticles:oai:doaj.org/article:8d1c5ae4dd664fa5adcb7275524c20f2 2023-05-15T15:09:27+02:00 Nuclear and kinetoplast DNA analyses reveal genetically complex Leishmania strains with hybrid and mito-nuclear discordance in Peru. Ahmed Tabbabi Abraham G Cáceres T Pershing Bustamante Chauca Chisato Seki Yanisa Choochartpong Daiki Mizushima Daisuke S Yamamoto Yoshihisa Hashiguchi Hirotomo Kato 2020-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008797 https://doaj.org/article/8d1c5ae4dd664fa5adcb7275524c20f2 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008797 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008797 https://doaj.org/article/8d1c5ae4dd664fa5adcb7275524c20f2 PLoS Neglected Tropical Diseases, Vol 14, Iss 10, p e0008797 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008797 2022-12-31T11:44:55Z Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the mannose phosphate isomerase (mpi) gene was applied to 134 skin samples collected from patients with cutaneous leishmaniasis (CL) in Peru for identification of the infecting parasite at the species level, and the results were compared with those of cytochrome b (cyt b) gene sequencing obtained in previous studies. Although most results (121/134) including 4 hybrids of Leishmania (Viannia) braziliensis and L. (V.) peruviana corresponded to those obtained in the previous study, PCR-RFLP analyses revealed the distribution of putative hybrid strains between L. (V.) peruviana and L. (V.) lainsoni in two samples, which has never been reported. Moreover, parasite strains showing discordance between kinetoplast and nuclear genes (kDNA and nDNA), so-called mito-nuclear discordance, were identified in 11 samples. Of these, six strains had the kDNAs of L. (V.) braziliensis or L. (V.) peruviana and nDNAs of L. (V.) guyanensis, and three strains had the kDNAs of L. (V.) shawi and nDNAs of L. (V.) braziliensis. The rest were identified as mito-nuclear discordance strains having kDNAs of L. (V.) braziliensis or L. (V.) peruviana and nDNAs of L. (V.) lainsoni, and kDNAs of L. (V.) lainsoni and nDNAs of L. (V.) braziliensis. The results demonstrate that Leishmania strains in Peru are genetically more complex than previously considered. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 10 e0008797 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ahmed Tabbabi Abraham G Cáceres T Pershing Bustamante Chauca Chisato Seki Yanisa Choochartpong Daiki Mizushima Daisuke S Yamamoto Yoshihisa Hashiguchi Hirotomo Kato Nuclear and kinetoplast DNA analyses reveal genetically complex Leishmania strains with hybrid and mito-nuclear discordance in Peru. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the mannose phosphate isomerase (mpi) gene was applied to 134 skin samples collected from patients with cutaneous leishmaniasis (CL) in Peru for identification of the infecting parasite at the species level, and the results were compared with those of cytochrome b (cyt b) gene sequencing obtained in previous studies. Although most results (121/134) including 4 hybrids of Leishmania (Viannia) braziliensis and L. (V.) peruviana corresponded to those obtained in the previous study, PCR-RFLP analyses revealed the distribution of putative hybrid strains between L. (V.) peruviana and L. (V.) lainsoni in two samples, which has never been reported. Moreover, parasite strains showing discordance between kinetoplast and nuclear genes (kDNA and nDNA), so-called mito-nuclear discordance, were identified in 11 samples. Of these, six strains had the kDNAs of L. (V.) braziliensis or L. (V.) peruviana and nDNAs of L. (V.) guyanensis, and three strains had the kDNAs of L. (V.) shawi and nDNAs of L. (V.) braziliensis. The rest were identified as mito-nuclear discordance strains having kDNAs of L. (V.) braziliensis or L. (V.) peruviana and nDNAs of L. (V.) lainsoni, and kDNAs of L. (V.) lainsoni and nDNAs of L. (V.) braziliensis. The results demonstrate that Leishmania strains in Peru are genetically more complex than previously considered. |
format |
Article in Journal/Newspaper |
author |
Ahmed Tabbabi Abraham G Cáceres T Pershing Bustamante Chauca Chisato Seki Yanisa Choochartpong Daiki Mizushima Daisuke S Yamamoto Yoshihisa Hashiguchi Hirotomo Kato |
author_facet |
Ahmed Tabbabi Abraham G Cáceres T Pershing Bustamante Chauca Chisato Seki Yanisa Choochartpong Daiki Mizushima Daisuke S Yamamoto Yoshihisa Hashiguchi Hirotomo Kato |
author_sort |
Ahmed Tabbabi |
title |
Nuclear and kinetoplast DNA analyses reveal genetically complex Leishmania strains with hybrid and mito-nuclear discordance in Peru. |
title_short |
Nuclear and kinetoplast DNA analyses reveal genetically complex Leishmania strains with hybrid and mito-nuclear discordance in Peru. |
title_full |
Nuclear and kinetoplast DNA analyses reveal genetically complex Leishmania strains with hybrid and mito-nuclear discordance in Peru. |
title_fullStr |
Nuclear and kinetoplast DNA analyses reveal genetically complex Leishmania strains with hybrid and mito-nuclear discordance in Peru. |
title_full_unstemmed |
Nuclear and kinetoplast DNA analyses reveal genetically complex Leishmania strains with hybrid and mito-nuclear discordance in Peru. |
title_sort |
nuclear and kinetoplast dna analyses reveal genetically complex leishmania strains with hybrid and mito-nuclear discordance in peru. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008797 https://doaj.org/article/8d1c5ae4dd664fa5adcb7275524c20f2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 10, p e0008797 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008797 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008797 https://doaj.org/article/8d1c5ae4dd664fa5adcb7275524c20f2 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008797 |
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PLOS Neglected Tropical Diseases |
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14 |
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10 |
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e0008797 |
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1766340650027974656 |