Presence of genes encoding enterotoxins in Staphylococcus aureus isolates recovered from food, food establishment surfaces and cases of foodborne diseases
ABSTRACT The aim of this study was to describe the microbiological characteristics and profile of genes encoding enterotoxins in 95 Staphylococcus aureus isolates obtained between April 2011 and December 2014 from foodstuffs, persons and surfaces of retail food stores. After microbiological identifi...
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ftdoajarticles:oai:doaj.org/article:8d1916cfbba9497fa3c62a2b2f7f1cdb 2024-09-09T19:27:16+00:00 Presence of genes encoding enterotoxins in Staphylococcus aureus isolates recovered from food, food establishment surfaces and cases of foodborne diseases Virginia Machado Lorena Pardo Dianna Cuello Guillermina Giudice Patricia Correa Luna Gustavo Varela Teresa Camou Felipe Schelotto 2020-02-01T00:00:00Z https://doi.org/10.1590/s1678-9946202062005 https://doaj.org/article/8d1916cfbba9497fa3c62a2b2f7f1cdb EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652020000100201&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946202062005 https://doaj.org/article/8d1916cfbba9497fa3c62a2b2f7f1cdb Revista do Instituto de Medicina Tropical de São Paulo, Vol 62 (2020) Staphylococcus aureus Enterotoxin genes Foods Antimicrobial resistance Multi Locus Sequence Typing Pulsed Field Gel Electrophoresis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1590/s1678-9946202062005 2024-08-05T17:49:30Z ABSTRACT The aim of this study was to describe the microbiological characteristics and profile of genes encoding enterotoxins in 95 Staphylococcus aureus isolates obtained between April 2011 and December 2014 from foodstuffs, persons and surfaces of retail food stores. After microbiological identification and antimicrobial susceptibility testing, polymerase chain reactions (PCR) were performed, targeting sea, seb, sec, sed and see genes that code for classical enterotoxins (ET) A-E, and three additional genes: seg , seh and sei , coding for so-called “new enterotoxins” G, H and I. The isolates were characterized by Pulsed Field Gel Electrophoresis (PFGE), and five selected isolates were further analyzed through Multi Locus Sequence Typing (MLST). It is noteworthy that 54.7% of the examined isolates harbored one or more of the investigated ET gene types. Most positive isolates carried more than one ET gene up to five types; seg was the most frequent ET gene, followed by sei. Five enterotoxin-coding isolates also coded for some antimicrobial resistance genes. Two of them, and four additional non-enterotoxic isolates carried erm genes expressing inducible clindamycin resistance. PFGE-types were numerous and diverse, even among enterotoxin-coding strains, because most isolates did not belong to known foodborne outbreaks and the sampling period was long. MLST profiles were also varied, and a new ST 3840 was described within this species. ST 88 and ST 72 enterotoxin-coding isolates have been identified in other regions in association with foodborne outbreaks. This manuscript reports the first systematic investigation of enterotoxin genes in S. aureus isolates obtained from foodstuffs and infected people in Uruguay. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Uruguay Revista do Instituto de Medicina Tropical de São Paulo 62 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Staphylococcus aureus Enterotoxin genes Foods Antimicrobial resistance Multi Locus Sequence Typing Pulsed Field Gel Electrophoresis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Staphylococcus aureus Enterotoxin genes Foods Antimicrobial resistance Multi Locus Sequence Typing Pulsed Field Gel Electrophoresis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Virginia Machado Lorena Pardo Dianna Cuello Guillermina Giudice Patricia Correa Luna Gustavo Varela Teresa Camou Felipe Schelotto Presence of genes encoding enterotoxins in Staphylococcus aureus isolates recovered from food, food establishment surfaces and cases of foodborne diseases |
topic_facet |
Staphylococcus aureus Enterotoxin genes Foods Antimicrobial resistance Multi Locus Sequence Typing Pulsed Field Gel Electrophoresis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
ABSTRACT The aim of this study was to describe the microbiological characteristics and profile of genes encoding enterotoxins in 95 Staphylococcus aureus isolates obtained between April 2011 and December 2014 from foodstuffs, persons and surfaces of retail food stores. After microbiological identification and antimicrobial susceptibility testing, polymerase chain reactions (PCR) were performed, targeting sea, seb, sec, sed and see genes that code for classical enterotoxins (ET) A-E, and three additional genes: seg , seh and sei , coding for so-called “new enterotoxins” G, H and I. The isolates were characterized by Pulsed Field Gel Electrophoresis (PFGE), and five selected isolates were further analyzed through Multi Locus Sequence Typing (MLST). It is noteworthy that 54.7% of the examined isolates harbored one or more of the investigated ET gene types. Most positive isolates carried more than one ET gene up to five types; seg was the most frequent ET gene, followed by sei. Five enterotoxin-coding isolates also coded for some antimicrobial resistance genes. Two of them, and four additional non-enterotoxic isolates carried erm genes expressing inducible clindamycin resistance. PFGE-types were numerous and diverse, even among enterotoxin-coding strains, because most isolates did not belong to known foodborne outbreaks and the sampling period was long. MLST profiles were also varied, and a new ST 3840 was described within this species. ST 88 and ST 72 enterotoxin-coding isolates have been identified in other regions in association with foodborne outbreaks. This manuscript reports the first systematic investigation of enterotoxin genes in S. aureus isolates obtained from foodstuffs and infected people in Uruguay. |
format |
Article in Journal/Newspaper |
author |
Virginia Machado Lorena Pardo Dianna Cuello Guillermina Giudice Patricia Correa Luna Gustavo Varela Teresa Camou Felipe Schelotto |
author_facet |
Virginia Machado Lorena Pardo Dianna Cuello Guillermina Giudice Patricia Correa Luna Gustavo Varela Teresa Camou Felipe Schelotto |
author_sort |
Virginia Machado |
title |
Presence of genes encoding enterotoxins in Staphylococcus aureus isolates recovered from food, food establishment surfaces and cases of foodborne diseases |
title_short |
Presence of genes encoding enterotoxins in Staphylococcus aureus isolates recovered from food, food establishment surfaces and cases of foodborne diseases |
title_full |
Presence of genes encoding enterotoxins in Staphylococcus aureus isolates recovered from food, food establishment surfaces and cases of foodborne diseases |
title_fullStr |
Presence of genes encoding enterotoxins in Staphylococcus aureus isolates recovered from food, food establishment surfaces and cases of foodborne diseases |
title_full_unstemmed |
Presence of genes encoding enterotoxins in Staphylococcus aureus isolates recovered from food, food establishment surfaces and cases of foodborne diseases |
title_sort |
presence of genes encoding enterotoxins in staphylococcus aureus isolates recovered from food, food establishment surfaces and cases of foodborne diseases |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2020 |
url |
https://doi.org/10.1590/s1678-9946202062005 https://doaj.org/article/8d1916cfbba9497fa3c62a2b2f7f1cdb |
geographic |
Arctic Uruguay |
geographic_facet |
Arctic Uruguay |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 62 (2020) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652020000100201&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946202062005 https://doaj.org/article/8d1916cfbba9497fa3c62a2b2f7f1cdb |
op_doi |
https://doi.org/10.1590/s1678-9946202062005 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
62 |
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1809896727659937792 |