Characterization of genes involved in ceramide metabolism in the Pacific oyster ( Crassostrea gigas )
Abstract Background The lipid signaling molecule, ceramide, is a key component of the vertebrate stress response, however, there is limited information concerning its role in invertebrate species. In order to identify genes involved in ceramide metabolism in bivalve molluscs, Pacific oyster genomic...
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ftdoajarticles:oai:doaj.org/article:8d13fc94917b4339b199e014f0ed0b4e 2023-05-15T15:58:14+02:00 Characterization of genes involved in ceramide metabolism in the Pacific oyster ( Crassostrea gigas ) Timmins-Schiffman Emma Roberts Steven 2012-09-01T00:00:00Z https://doi.org/10.1186/1756-0500-5-502 https://doaj.org/article/8d13fc94917b4339b199e014f0ed0b4e EN eng BMC http://www.biomedcentral.com/1756-0500/5/502 https://doaj.org/toc/1756-0500 doi:10.1186/1756-0500-5-502 1756-0500 https://doaj.org/article/8d13fc94917b4339b199e014f0ed0b4e BMC Research Notes, Vol 5, Iss 1, p 502 (2012) Oyster Crassostrea gigas Stress Ceramide Bacteria Medicine R Biology (General) QH301-705.5 Science (General) Q1-390 article 2012 ftdoajarticles https://doi.org/10.1186/1756-0500-5-502 2022-12-31T00:09:57Z Abstract Background The lipid signaling molecule, ceramide, is a key component of the vertebrate stress response, however, there is limited information concerning its role in invertebrate species. In order to identify genes involved in ceramide metabolism in bivalve molluscs, Pacific oyster genomic resources were examined for genes associated with ceramide metabolism and signaling. Results Several genes were identified including full-length sequences characterized for serine palmitoyltransferase-1, 3-ketodihydrosphingosine reductase, acid ceramidase, and ceramide glucosyltransferase . Genes involved in ceramide synthesis and metabolism are conserved across taxa in both form and function. Expression analysis as assessed by quantitative PCR indicated all genes were expressed at high levels in gill tissue. The role of the ceramide pathway genes in the invertebrate stress response was also explored by measuring expression levels in adult oysters exposed to Vibrio vulnificus . Two genes demonstrated increased expression during the bacterial challenge: a gene involved in hydrolytic breakdown of ceramide ( acid ceramidase ) and a gene involved in de novo generation of ceramide ( 3-ketodihydrosphingosine reductase ), suggesting a possible role of ceramide in the invertebrate stress and immune responses. Conclusions In silico and laboratory results support that Pacific oysters have the basic components of the ceramide metabolism pathway. These results also indicate that ceramide may have analogous functions in vertebrates and invertebrates. The gene expression pattern of acid ceramidase and 3-kethodihydrosphingosine reductase in response to bacterial exposure especially supports that ceramide and sphingolipid metabolism may be involved in the oyster’s stress and/or immune responses. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific BMC Research Notes 5 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Oyster Crassostrea gigas Stress Ceramide Bacteria Medicine R Biology (General) QH301-705.5 Science (General) Q1-390 |
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Oyster Crassostrea gigas Stress Ceramide Bacteria Medicine R Biology (General) QH301-705.5 Science (General) Q1-390 Timmins-Schiffman Emma Roberts Steven Characterization of genes involved in ceramide metabolism in the Pacific oyster ( Crassostrea gigas ) |
topic_facet |
Oyster Crassostrea gigas Stress Ceramide Bacteria Medicine R Biology (General) QH301-705.5 Science (General) Q1-390 |
description |
Abstract Background The lipid signaling molecule, ceramide, is a key component of the vertebrate stress response, however, there is limited information concerning its role in invertebrate species. In order to identify genes involved in ceramide metabolism in bivalve molluscs, Pacific oyster genomic resources were examined for genes associated with ceramide metabolism and signaling. Results Several genes were identified including full-length sequences characterized for serine palmitoyltransferase-1, 3-ketodihydrosphingosine reductase, acid ceramidase, and ceramide glucosyltransferase . Genes involved in ceramide synthesis and metabolism are conserved across taxa in both form and function. Expression analysis as assessed by quantitative PCR indicated all genes were expressed at high levels in gill tissue. The role of the ceramide pathway genes in the invertebrate stress response was also explored by measuring expression levels in adult oysters exposed to Vibrio vulnificus . Two genes demonstrated increased expression during the bacterial challenge: a gene involved in hydrolytic breakdown of ceramide ( acid ceramidase ) and a gene involved in de novo generation of ceramide ( 3-ketodihydrosphingosine reductase ), suggesting a possible role of ceramide in the invertebrate stress and immune responses. Conclusions In silico and laboratory results support that Pacific oysters have the basic components of the ceramide metabolism pathway. These results also indicate that ceramide may have analogous functions in vertebrates and invertebrates. The gene expression pattern of acid ceramidase and 3-kethodihydrosphingosine reductase in response to bacterial exposure especially supports that ceramide and sphingolipid metabolism may be involved in the oyster’s stress and/or immune responses. |
format |
Article in Journal/Newspaper |
author |
Timmins-Schiffman Emma Roberts Steven |
author_facet |
Timmins-Schiffman Emma Roberts Steven |
author_sort |
Timmins-Schiffman Emma |
title |
Characterization of genes involved in ceramide metabolism in the Pacific oyster ( Crassostrea gigas ) |
title_short |
Characterization of genes involved in ceramide metabolism in the Pacific oyster ( Crassostrea gigas ) |
title_full |
Characterization of genes involved in ceramide metabolism in the Pacific oyster ( Crassostrea gigas ) |
title_fullStr |
Characterization of genes involved in ceramide metabolism in the Pacific oyster ( Crassostrea gigas ) |
title_full_unstemmed |
Characterization of genes involved in ceramide metabolism in the Pacific oyster ( Crassostrea gigas ) |
title_sort |
characterization of genes involved in ceramide metabolism in the pacific oyster ( crassostrea gigas ) |
publisher |
BMC |
publishDate |
2012 |
url |
https://doi.org/10.1186/1756-0500-5-502 https://doaj.org/article/8d13fc94917b4339b199e014f0ed0b4e |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
BMC Research Notes, Vol 5, Iss 1, p 502 (2012) |
op_relation |
http://www.biomedcentral.com/1756-0500/5/502 https://doaj.org/toc/1756-0500 doi:10.1186/1756-0500-5-502 1756-0500 https://doaj.org/article/8d13fc94917b4339b199e014f0ed0b4e |
op_doi |
https://doi.org/10.1186/1756-0500-5-502 |
container_title |
BMC Research Notes |
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5 |
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1 |
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1766393961827532800 |