Combining Wolbachia-induced sterility and virus protection to fight Aedes albopictus-borne viruses.
Among the strategies targeting vector control, the exploitation of the endosymbiont Wolbachia to produce sterile males and/or invasive females with reduced vector competence seems to be promising. A new Aedes albopictus transinfection (ARwP-M) was generated by introducing wMel Wolbachia in the ARwP...
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ftdoajarticles:oai:doaj.org/article:8cfd191ad6a94128a56e1a2b491f5f57 2023-05-15T15:16:19+02:00 Combining Wolbachia-induced sterility and virus protection to fight Aedes albopictus-borne viruses. Riccardo Moretti Pei-Shi Yen Vincent Houé Elena Lampazzi Angiola Desiderio Anna-Bella Failloux Maurizio Calvitti 2018-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006626 https://doaj.org/article/8cfd191ad6a94128a56e1a2b491f5f57 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC6066253?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006626 https://doaj.org/article/8cfd191ad6a94128a56e1a2b491f5f57 PLoS Neglected Tropical Diseases, Vol 12, Iss 7, p e0006626 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006626 2022-12-31T11:11:42Z Among the strategies targeting vector control, the exploitation of the endosymbiont Wolbachia to produce sterile males and/or invasive females with reduced vector competence seems to be promising. A new Aedes albopictus transinfection (ARwP-M) was generated by introducing wMel Wolbachia in the ARwP line which had been established previously by replacing wAlbA and wAlbB Wolbachia with the wPip strain. Various infection and fitness parameters were studied by comparing ARwP-M, ARwP and wild-type (SANG population) Ae. albopictus sharing the same genetic background. Moreover, the vector competence of ARwP-M related to chikungunya, dengue and zika viruses was evaluated in comparison with ARwP. ARwP-M showed a 100% rate of maternal inheritance of wMel and wPip Wolbachia. Survival, female fecundity and egg fertility did not show to differ between the three Ae. albopictus lines. Crosses between ARwP-M males and SANG females were fully unfertile regardless of male age while egg hatch in reverse crosses increased from 0 to about 17% with SANG males aging from 3 to 17 days. When competing with SANG males for SANG females, ARwP-M males induced a level of sterility significantly higher than that expected for an equal mating competitiveness (mean Fried index of 1.71 instead of 1). The overall Wolbachia density in ARwP-M females was about 15 fold higher than in ARwP, mostly due to the wMel infection. This feature corresponded to a strongly reduced vector competence for chikungunya and dengue viruses (in both cases, 5 and 0% rates of transmission at 14 and 21 days post infection) with respect to ARwP females. Results regarding Zika virus did not highlight significant differences between ARwP-M and ARwP. However, none of the tested ARwP-M females was capable at transmitting ZIKV. These findings are expected to promote the exploitation of Wolbachia to suppress the wild-type Ae. albopictus populations. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 12 7 e0006626 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Riccardo Moretti Pei-Shi Yen Vincent Houé Elena Lampazzi Angiola Desiderio Anna-Bella Failloux Maurizio Calvitti Combining Wolbachia-induced sterility and virus protection to fight Aedes albopictus-borne viruses. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Among the strategies targeting vector control, the exploitation of the endosymbiont Wolbachia to produce sterile males and/or invasive females with reduced vector competence seems to be promising. A new Aedes albopictus transinfection (ARwP-M) was generated by introducing wMel Wolbachia in the ARwP line which had been established previously by replacing wAlbA and wAlbB Wolbachia with the wPip strain. Various infection and fitness parameters were studied by comparing ARwP-M, ARwP and wild-type (SANG population) Ae. albopictus sharing the same genetic background. Moreover, the vector competence of ARwP-M related to chikungunya, dengue and zika viruses was evaluated in comparison with ARwP. ARwP-M showed a 100% rate of maternal inheritance of wMel and wPip Wolbachia. Survival, female fecundity and egg fertility did not show to differ between the three Ae. albopictus lines. Crosses between ARwP-M males and SANG females were fully unfertile regardless of male age while egg hatch in reverse crosses increased from 0 to about 17% with SANG males aging from 3 to 17 days. When competing with SANG males for SANG females, ARwP-M males induced a level of sterility significantly higher than that expected for an equal mating competitiveness (mean Fried index of 1.71 instead of 1). The overall Wolbachia density in ARwP-M females was about 15 fold higher than in ARwP, mostly due to the wMel infection. This feature corresponded to a strongly reduced vector competence for chikungunya and dengue viruses (in both cases, 5 and 0% rates of transmission at 14 and 21 days post infection) with respect to ARwP females. Results regarding Zika virus did not highlight significant differences between ARwP-M and ARwP. However, none of the tested ARwP-M females was capable at transmitting ZIKV. These findings are expected to promote the exploitation of Wolbachia to suppress the wild-type Ae. albopictus populations. |
format |
Article in Journal/Newspaper |
author |
Riccardo Moretti Pei-Shi Yen Vincent Houé Elena Lampazzi Angiola Desiderio Anna-Bella Failloux Maurizio Calvitti |
author_facet |
Riccardo Moretti Pei-Shi Yen Vincent Houé Elena Lampazzi Angiola Desiderio Anna-Bella Failloux Maurizio Calvitti |
author_sort |
Riccardo Moretti |
title |
Combining Wolbachia-induced sterility and virus protection to fight Aedes albopictus-borne viruses. |
title_short |
Combining Wolbachia-induced sterility and virus protection to fight Aedes albopictus-borne viruses. |
title_full |
Combining Wolbachia-induced sterility and virus protection to fight Aedes albopictus-borne viruses. |
title_fullStr |
Combining Wolbachia-induced sterility and virus protection to fight Aedes albopictus-borne viruses. |
title_full_unstemmed |
Combining Wolbachia-induced sterility and virus protection to fight Aedes albopictus-borne viruses. |
title_sort |
combining wolbachia-induced sterility and virus protection to fight aedes albopictus-borne viruses. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006626 https://doaj.org/article/8cfd191ad6a94128a56e1a2b491f5f57 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 7, p e0006626 (2018) |
op_relation |
http://europepmc.org/articles/PMC6066253?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006626 https://doaj.org/article/8cfd191ad6a94128a56e1a2b491f5f57 |
op_doi |
https://doi.org/10.1371/journal.pntd.0006626 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
12 |
container_issue |
7 |
container_start_page |
e0006626 |
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1766346612656832512 |