Neonatal immune response of Brazilian beef cattle to vaccination with Clostridium botulinum toxoids types C and D by indirect ELISA
Types C and D strains of Clostridium botulinum are commonly related to avian and mammalian botulism. Although there are numerous vaccine recommendations, little research has been conducted to indicate the real effectiveness of vaccine timing or the ideal immunization protocol for young beef calves....
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:8cfce7e249274f5c9c325c180157f634 2023-05-15T15:15:24+02:00 Neonatal immune response of Brazilian beef cattle to vaccination with Clostridium botulinum toxoids types C and D by indirect ELISA VCM Curci AHC Nogueira FLC Nobrega RF Araujo SHV Perri TC Cardoso IS Dutra 2010-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992010000300018 https://doaj.org/article/8cfce7e249274f5c9c325c180157f634 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300018 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992010000300018 1678-9199 https://doaj.org/article/8cfce7e249274f5c9c325c180157f634 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 16, Iss 3, Pp 509-513 (2010) Clostridium botulinum neonatal immunity cattle botulism Brazil Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2010 ftdoajarticles https://doi.org/10.1590/S1678-91992010000300018 2022-12-31T03:30:21Z Types C and D strains of Clostridium botulinum are commonly related to avian and mammalian botulism. Although there are numerous vaccine recommendations, little research has been conducted to indicate the real effectiveness of vaccine timing or the ideal immunization protocol for young beef calves. Four commercially available vaccines, two bivalent (Clostridium botulinum types C and D; vaccines 1 and 2) and two polyvalent (all Clostridium spp. including Clostridium botulinum types C and D; vaccines 3 and 4), that are currently used in Brazilian herds, were tested in order to verify the maternal immune response. One hundred cows, divided into four vaccinated groups and one unvaccinated group, were given a two-dose subcutaneous immunization, at day zero, followed by a second dose given at 42 days post-vaccination, which corresponded to 40 days before birth. Serum samples (n = 75) were collected only from healthy neonatal calves at 0, 7, 45 and 90 days post-calving (DPC) and subjected to indirect ELISA using the purified C and D holotoxins as capture antigens. The serological profile showed that all vaccines were able to induce a satisfactory neonatal immune response to both holotoxins at 7 DPC. However, at 45 and 90 DPC, a significant reduction (p < 0.05) was observed in the antibody level against C and D holotoxins in all tested vaccines. Neonatal immunization in calves is compromised by significant levels of maternal antibodies so that the necessity of planning a calf vaccination program involves assessment of disease risks at the production site. Finally, our findings represent the first demonstration of maternal immunity transferred to neonatal beef calves, including immunity levels after vaccination against Clostridium botulinum toxoids C and D. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 16 3 509 513 |
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Clostridium botulinum neonatal immunity cattle botulism Brazil Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
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Clostridium botulinum neonatal immunity cattle botulism Brazil Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 VCM Curci AHC Nogueira FLC Nobrega RF Araujo SHV Perri TC Cardoso IS Dutra Neonatal immune response of Brazilian beef cattle to vaccination with Clostridium botulinum toxoids types C and D by indirect ELISA |
topic_facet |
Clostridium botulinum neonatal immunity cattle botulism Brazil Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Types C and D strains of Clostridium botulinum are commonly related to avian and mammalian botulism. Although there are numerous vaccine recommendations, little research has been conducted to indicate the real effectiveness of vaccine timing or the ideal immunization protocol for young beef calves. Four commercially available vaccines, two bivalent (Clostridium botulinum types C and D; vaccines 1 and 2) and two polyvalent (all Clostridium spp. including Clostridium botulinum types C and D; vaccines 3 and 4), that are currently used in Brazilian herds, were tested in order to verify the maternal immune response. One hundred cows, divided into four vaccinated groups and one unvaccinated group, were given a two-dose subcutaneous immunization, at day zero, followed by a second dose given at 42 days post-vaccination, which corresponded to 40 days before birth. Serum samples (n = 75) were collected only from healthy neonatal calves at 0, 7, 45 and 90 days post-calving (DPC) and subjected to indirect ELISA using the purified C and D holotoxins as capture antigens. The serological profile showed that all vaccines were able to induce a satisfactory neonatal immune response to both holotoxins at 7 DPC. However, at 45 and 90 DPC, a significant reduction (p < 0.05) was observed in the antibody level against C and D holotoxins in all tested vaccines. Neonatal immunization in calves is compromised by significant levels of maternal antibodies so that the necessity of planning a calf vaccination program involves assessment of disease risks at the production site. Finally, our findings represent the first demonstration of maternal immunity transferred to neonatal beef calves, including immunity levels after vaccination against Clostridium botulinum toxoids C and D. |
format |
Article in Journal/Newspaper |
author |
VCM Curci AHC Nogueira FLC Nobrega RF Araujo SHV Perri TC Cardoso IS Dutra |
author_facet |
VCM Curci AHC Nogueira FLC Nobrega RF Araujo SHV Perri TC Cardoso IS Dutra |
author_sort |
VCM Curci |
title |
Neonatal immune response of Brazilian beef cattle to vaccination with Clostridium botulinum toxoids types C and D by indirect ELISA |
title_short |
Neonatal immune response of Brazilian beef cattle to vaccination with Clostridium botulinum toxoids types C and D by indirect ELISA |
title_full |
Neonatal immune response of Brazilian beef cattle to vaccination with Clostridium botulinum toxoids types C and D by indirect ELISA |
title_fullStr |
Neonatal immune response of Brazilian beef cattle to vaccination with Clostridium botulinum toxoids types C and D by indirect ELISA |
title_full_unstemmed |
Neonatal immune response of Brazilian beef cattle to vaccination with Clostridium botulinum toxoids types C and D by indirect ELISA |
title_sort |
neonatal immune response of brazilian beef cattle to vaccination with clostridium botulinum toxoids types c and d by indirect elisa |
publisher |
SciELO |
publishDate |
2010 |
url |
https://doi.org/10.1590/S1678-91992010000300018 https://doaj.org/article/8cfce7e249274f5c9c325c180157f634 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 16, Iss 3, Pp 509-513 (2010) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300018 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992010000300018 1678-9199 https://doaj.org/article/8cfce7e249274f5c9c325c180157f634 |
op_doi |
https://doi.org/10.1590/S1678-91992010000300018 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
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16 |
container_issue |
3 |
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509 |
op_container_end_page |
513 |
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1766345764303273984 |