Comparison of proteins, lethality and immunogenic compounds of Androctonus crassicauda (Olivier, 1807) (Scorpiones: Buthidae) venom obtained by different methods
Scorpions are venomous arthropods of the class Arachnida and are considered relatives of spiders, ticks and mites. There are approximately 1,500 species of scorpions worldwide, which are characterized by an elongated body and a segmented tail that ends in a venomous stinger. No specific treatment is...
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ftdoajarticles:oai:doaj.org/article:8ce3f48381b646e1ad7082dd063728aa 2023-05-15T15:07:47+02:00 Comparison of proteins, lethality and immunogenic compounds of Androctonus crassicauda (Olivier, 1807) (Scorpiones: Buthidae) venom obtained by different methods O. Ozkan S. Kar E. Güven G. Ergun 2007-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992007000400013 https://doaj.org/article/8ce3f48381b646e1ad7082dd063728aa EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400013 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992007000400013 1678-9199 https://doaj.org/article/8ce3f48381b646e1ad7082dd063728aa Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 13, Iss 4, Pp 844-856 (2007) scorpion venoms Androctonus crassicauda gland maceration electric stimulation immunogenicity lethality Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2007 ftdoajarticles https://doi.org/10.1590/S1678-91992007000400013 2022-12-31T00:20:15Z Scorpions are venomous arthropods of the class Arachnida and are considered relatives of spiders, ticks and mites. There are approximately 1,500 species of scorpions worldwide, which are characterized by an elongated body and a segmented tail that ends in a venomous stinger. No specific treatment is available for scorpion envenomation, except for the use of antivenom. The current study aimed at comparing protein content and lethality of Androctonus crassicauda venom extracted by two different methods (electric stimulation and maceration of telsons). The LD50 calculated by probit analysis was 1.1mg/kg for venom obtained by electric stimulation and 39.19mg/kg for venom obtained by maceration of telsons. In the electrophoretic analysis, protein bands of the venom sample obtained by electric stimulation were between 12 and 53kDa (total: five bands), and those of venom extracted by maceration appeared as multiple protein bands, relative to the other venom sample. Low-molecular-weight proteins, revealed by western blotting, played an important immunogenic role in the production of antivenom. Lethality and protein levels varied according to the extraction method; venom obtained by the maceration technique showed lower toxicity than that obtained by electric stimulation. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 13 4 844 856 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
scorpion venoms Androctonus crassicauda gland maceration electric stimulation immunogenicity lethality Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
scorpion venoms Androctonus crassicauda gland maceration electric stimulation immunogenicity lethality Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 O. Ozkan S. Kar E. Güven G. Ergun Comparison of proteins, lethality and immunogenic compounds of Androctonus crassicauda (Olivier, 1807) (Scorpiones: Buthidae) venom obtained by different methods |
topic_facet |
scorpion venoms Androctonus crassicauda gland maceration electric stimulation immunogenicity lethality Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Scorpions are venomous arthropods of the class Arachnida and are considered relatives of spiders, ticks and mites. There are approximately 1,500 species of scorpions worldwide, which are characterized by an elongated body and a segmented tail that ends in a venomous stinger. No specific treatment is available for scorpion envenomation, except for the use of antivenom. The current study aimed at comparing protein content and lethality of Androctonus crassicauda venom extracted by two different methods (electric stimulation and maceration of telsons). The LD50 calculated by probit analysis was 1.1mg/kg for venom obtained by electric stimulation and 39.19mg/kg for venom obtained by maceration of telsons. In the electrophoretic analysis, protein bands of the venom sample obtained by electric stimulation were between 12 and 53kDa (total: five bands), and those of venom extracted by maceration appeared as multiple protein bands, relative to the other venom sample. Low-molecular-weight proteins, revealed by western blotting, played an important immunogenic role in the production of antivenom. Lethality and protein levels varied according to the extraction method; venom obtained by the maceration technique showed lower toxicity than that obtained by electric stimulation. |
format |
Article in Journal/Newspaper |
author |
O. Ozkan S. Kar E. Güven G. Ergun |
author_facet |
O. Ozkan S. Kar E. Güven G. Ergun |
author_sort |
O. Ozkan |
title |
Comparison of proteins, lethality and immunogenic compounds of Androctonus crassicauda (Olivier, 1807) (Scorpiones: Buthidae) venom obtained by different methods |
title_short |
Comparison of proteins, lethality and immunogenic compounds of Androctonus crassicauda (Olivier, 1807) (Scorpiones: Buthidae) venom obtained by different methods |
title_full |
Comparison of proteins, lethality and immunogenic compounds of Androctonus crassicauda (Olivier, 1807) (Scorpiones: Buthidae) venom obtained by different methods |
title_fullStr |
Comparison of proteins, lethality and immunogenic compounds of Androctonus crassicauda (Olivier, 1807) (Scorpiones: Buthidae) venom obtained by different methods |
title_full_unstemmed |
Comparison of proteins, lethality and immunogenic compounds of Androctonus crassicauda (Olivier, 1807) (Scorpiones: Buthidae) venom obtained by different methods |
title_sort |
comparison of proteins, lethality and immunogenic compounds of androctonus crassicauda (olivier, 1807) (scorpiones: buthidae) venom obtained by different methods |
publisher |
SciELO |
publishDate |
2007 |
url |
https://doi.org/10.1590/S1678-91992007000400013 https://doaj.org/article/8ce3f48381b646e1ad7082dd063728aa |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 13, Iss 4, Pp 844-856 (2007) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400013 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992007000400013 1678-9199 https://doaj.org/article/8ce3f48381b646e1ad7082dd063728aa |
op_doi |
https://doi.org/10.1590/S1678-91992007000400013 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
13 |
container_issue |
4 |
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844 |
op_container_end_page |
856 |
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