Prevalence and Genotyping of Anaplasma phagocytophilum Strains from Wild Animals, European Bison ( Bison bonasus ) and Eurasian Moose ( Alces alces ) in Poland
Wild large ungulates, like European bison ( Bison bonasus ) and Eurasian moose ( Alces alces ), form an important part of the circulation of Anaplasma phagocytophilum , a Gram-negative, intracellular, tick-transmitted bacterium, in the natural environment. Bison and moose tissue samples were subject...
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2022
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ftdoajarticles:oai:doaj.org/article:8cac32c2a1ac4ee5b6ea37b540274176 2023-05-15T13:12:41+02:00 Prevalence and Genotyping of Anaplasma phagocytophilum Strains from Wild Animals, European Bison ( Bison bonasus ) and Eurasian Moose ( Alces alces ) in Poland Anna W. Myczka Stanisław Kaczor Katarzyna Filip-Hutsch Michał Czopowicz Elwira Plis-Kuprianowicz Zdzisław Laskowski 2022-05-01T00:00:00Z https://doi.org/10.3390/ani12091222 https://doaj.org/article/8cac32c2a1ac4ee5b6ea37b540274176 EN eng MDPI AG https://www.mdpi.com/2076-2615/12/9/1222 https://doaj.org/toc/2076-2615 doi:10.3390/ani12091222 2076-2615 https://doaj.org/article/8cac32c2a1ac4ee5b6ea37b540274176 Animals, Vol 12, Iss 1222, p 1222 (2022) Anaplasma phagocytophilum 16S rDNA groEL ankA Bison bonasus Alces alces Veterinary medicine SF600-1100 Zoology QL1-991 article 2022 ftdoajarticles https://doi.org/10.3390/ani12091222 2022-12-30T22:37:57Z Wild large ungulates, like European bison ( Bison bonasus ) and Eurasian moose ( Alces alces ), form an important part of the circulation of Anaplasma phagocytophilum , a Gram-negative, intracellular, tick-transmitted bacterium, in the natural environment. Bison and moose tissue samples were subjected to 16S rDNA, groEL and ankA partial gene marker amplification with specific primers using various variants of PCR. Out of 42 examined individuals, Anaplasma sp. were detected in 4/13 Eurasian moose (31%) and 7/29 European bison (24%). In addition, 12 groEL and 5 ankA partial gene positive samples were obtained from the examined animals. The phylogenetic analysis of the groEL partial gene classified samples from European bison to ecotype I, and samples from Eurasian moose to ecotype I and II; the analysis of the ankA partial gene assigned the samples to clusters I and IV. This study extends knowledge about A. phagocytophilum in wild large ungulates in Poland. This is the first report about the occurrence of Anaplasma sp. in one of the largest populations of free living European bison in the world. Our findings confirm that strains of A. phagocytophilum from Bison bonasus and Alces alces may constitute a natural reservoir of pathogenic HGA Anaplasma strains. Article in Journal/Newspaper Alces alces Directory of Open Access Journals: DOAJ Articles Animals 12 9 1222 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Anaplasma phagocytophilum 16S rDNA groEL ankA Bison bonasus Alces alces Veterinary medicine SF600-1100 Zoology QL1-991 |
spellingShingle |
Anaplasma phagocytophilum 16S rDNA groEL ankA Bison bonasus Alces alces Veterinary medicine SF600-1100 Zoology QL1-991 Anna W. Myczka Stanisław Kaczor Katarzyna Filip-Hutsch Michał Czopowicz Elwira Plis-Kuprianowicz Zdzisław Laskowski Prevalence and Genotyping of Anaplasma phagocytophilum Strains from Wild Animals, European Bison ( Bison bonasus ) and Eurasian Moose ( Alces alces ) in Poland |
topic_facet |
Anaplasma phagocytophilum 16S rDNA groEL ankA Bison bonasus Alces alces Veterinary medicine SF600-1100 Zoology QL1-991 |
description |
Wild large ungulates, like European bison ( Bison bonasus ) and Eurasian moose ( Alces alces ), form an important part of the circulation of Anaplasma phagocytophilum , a Gram-negative, intracellular, tick-transmitted bacterium, in the natural environment. Bison and moose tissue samples were subjected to 16S rDNA, groEL and ankA partial gene marker amplification with specific primers using various variants of PCR. Out of 42 examined individuals, Anaplasma sp. were detected in 4/13 Eurasian moose (31%) and 7/29 European bison (24%). In addition, 12 groEL and 5 ankA partial gene positive samples were obtained from the examined animals. The phylogenetic analysis of the groEL partial gene classified samples from European bison to ecotype I, and samples from Eurasian moose to ecotype I and II; the analysis of the ankA partial gene assigned the samples to clusters I and IV. This study extends knowledge about A. phagocytophilum in wild large ungulates in Poland. This is the first report about the occurrence of Anaplasma sp. in one of the largest populations of free living European bison in the world. Our findings confirm that strains of A. phagocytophilum from Bison bonasus and Alces alces may constitute a natural reservoir of pathogenic HGA Anaplasma strains. |
format |
Article in Journal/Newspaper |
author |
Anna W. Myczka Stanisław Kaczor Katarzyna Filip-Hutsch Michał Czopowicz Elwira Plis-Kuprianowicz Zdzisław Laskowski |
author_facet |
Anna W. Myczka Stanisław Kaczor Katarzyna Filip-Hutsch Michał Czopowicz Elwira Plis-Kuprianowicz Zdzisław Laskowski |
author_sort |
Anna W. Myczka |
title |
Prevalence and Genotyping of Anaplasma phagocytophilum Strains from Wild Animals, European Bison ( Bison bonasus ) and Eurasian Moose ( Alces alces ) in Poland |
title_short |
Prevalence and Genotyping of Anaplasma phagocytophilum Strains from Wild Animals, European Bison ( Bison bonasus ) and Eurasian Moose ( Alces alces ) in Poland |
title_full |
Prevalence and Genotyping of Anaplasma phagocytophilum Strains from Wild Animals, European Bison ( Bison bonasus ) and Eurasian Moose ( Alces alces ) in Poland |
title_fullStr |
Prevalence and Genotyping of Anaplasma phagocytophilum Strains from Wild Animals, European Bison ( Bison bonasus ) and Eurasian Moose ( Alces alces ) in Poland |
title_full_unstemmed |
Prevalence and Genotyping of Anaplasma phagocytophilum Strains from Wild Animals, European Bison ( Bison bonasus ) and Eurasian Moose ( Alces alces ) in Poland |
title_sort |
prevalence and genotyping of anaplasma phagocytophilum strains from wild animals, european bison ( bison bonasus ) and eurasian moose ( alces alces ) in poland |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/ani12091222 https://doaj.org/article/8cac32c2a1ac4ee5b6ea37b540274176 |
genre |
Alces alces |
genre_facet |
Alces alces |
op_source |
Animals, Vol 12, Iss 1222, p 1222 (2022) |
op_relation |
https://www.mdpi.com/2076-2615/12/9/1222 https://doaj.org/toc/2076-2615 doi:10.3390/ani12091222 2076-2615 https://doaj.org/article/8cac32c2a1ac4ee5b6ea37b540274176 |
op_doi |
https://doi.org/10.3390/ani12091222 |
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Animals |
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12 |
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9 |
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1222 |
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1766253446658260992 |