Molecular identification of Sarcocystis halieti n. sp., Sarcocystis lari and Sarcocystis truncata in the intestine of a white-tailed sea eagle (Haliaeetus albicilla) in Norway
An emaciated white-tailed sea eagle (Haliaeetus albicilla) from Western Norway was found and nursed briefly before it died. The necropsy revealed that the principal cause of death was an inflammation and occlusion of the bile ducts. A secondary finding was the presence in the intestinal mucosa of nu...
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ftdoajarticles:oai:doaj.org/article:8c9e1ae06eb74cbc9606f34a19ef538f 2023-05-15T16:32:40+02:00 Molecular identification of Sarcocystis halieti n. sp., Sarcocystis lari and Sarcocystis truncata in the intestine of a white-tailed sea eagle (Haliaeetus albicilla) in Norway Bjørn Gjerde Turid Vikøren Inger Sofie Hamnes 2018-04-01T00:00:00Z https://doi.org/10.1016/j.ijppaw.2017.12.001 https://doaj.org/article/8c9e1ae06eb74cbc9606f34a19ef538f EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2213224417301335 https://doaj.org/toc/2213-2244 2213-2244 doi:10.1016/j.ijppaw.2017.12.001 https://doaj.org/article/8c9e1ae06eb74cbc9606f34a19ef538f International Journal for Parasitology: Parasites and Wildlife, Vol 7, Iss 1, Pp 1-11 (2018) Zoology QL1-991 article 2018 ftdoajarticles https://doi.org/10.1016/j.ijppaw.2017.12.001 2022-12-31T14:25:19Z An emaciated white-tailed sea eagle (Haliaeetus albicilla) from Western Norway was found and nursed briefly before it died. The necropsy revealed that the principal cause of death was an inflammation and occlusion of the bile ducts. A secondary finding was the presence in the intestinal mucosa of numerous sporulated Sarcocystis oocysts measuring 21.8–22.8 × 16.0–17.0 μm. The aim of this study was to identify these oocysts to species level using molecular methods. Genomic DNA was extracted from 10 mucosal scrapings containing oocysts and subjected to PCR amplification and sequencing of four DNA regions: the 18S and 28S rRNA genes, the ITS1 region and the cox1 gene. DNA of three previously known Sarcocystis spp. was identified, but only two of these, Sarcocystis halieti n. sp. and Sarcocystis lari, both employing sea birds as intermediate hosts, were considered to have used the sea eagle as a definitive host and to have formed oocysts in its intestine. The third species found, Sarcocystis truncata, employs red deer as intermediate hosts and seems to use felids as definitive hosts based on its phylogenetic position and prevalence. The sea eagle had probably recently ingested portions of one of the latter hosts (red deer or cat/lynx) containing stages (sarcocysts/oocysts) and thus DNA of S. truncata. The species S. halieti and S. lari could only be unambiguously separated from their most closely related congeners on the basis of their ITS1 sequences. This is the first report of Sarcocystis oocysts in sea eagles and the first identification to species level of Sarcocystis oocysts in any type of eagle. The sea eagle also acted as intermediate host of an unidentified Sarcocystis spp. as evidenced by the finding of six thin-walled sarcocysts in a histological section of cardiac muscle. Keywords: Sarcocystis, Haliaeetus albicilla, Oocysts, ITS1, Cox1, Phylogeny Article in Journal/Newspaper Haliaeetus albicilla Lynx Directory of Open Access Journals: DOAJ Articles Norway International Journal for Parasitology: Parasites and Wildlife 7 1 1 11 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Zoology QL1-991 |
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Zoology QL1-991 Bjørn Gjerde Turid Vikøren Inger Sofie Hamnes Molecular identification of Sarcocystis halieti n. sp., Sarcocystis lari and Sarcocystis truncata in the intestine of a white-tailed sea eagle (Haliaeetus albicilla) in Norway |
topic_facet |
Zoology QL1-991 |
description |
An emaciated white-tailed sea eagle (Haliaeetus albicilla) from Western Norway was found and nursed briefly before it died. The necropsy revealed that the principal cause of death was an inflammation and occlusion of the bile ducts. A secondary finding was the presence in the intestinal mucosa of numerous sporulated Sarcocystis oocysts measuring 21.8–22.8 × 16.0–17.0 μm. The aim of this study was to identify these oocysts to species level using molecular methods. Genomic DNA was extracted from 10 mucosal scrapings containing oocysts and subjected to PCR amplification and sequencing of four DNA regions: the 18S and 28S rRNA genes, the ITS1 region and the cox1 gene. DNA of three previously known Sarcocystis spp. was identified, but only two of these, Sarcocystis halieti n. sp. and Sarcocystis lari, both employing sea birds as intermediate hosts, were considered to have used the sea eagle as a definitive host and to have formed oocysts in its intestine. The third species found, Sarcocystis truncata, employs red deer as intermediate hosts and seems to use felids as definitive hosts based on its phylogenetic position and prevalence. The sea eagle had probably recently ingested portions of one of the latter hosts (red deer or cat/lynx) containing stages (sarcocysts/oocysts) and thus DNA of S. truncata. The species S. halieti and S. lari could only be unambiguously separated from their most closely related congeners on the basis of their ITS1 sequences. This is the first report of Sarcocystis oocysts in sea eagles and the first identification to species level of Sarcocystis oocysts in any type of eagle. The sea eagle also acted as intermediate host of an unidentified Sarcocystis spp. as evidenced by the finding of six thin-walled sarcocysts in a histological section of cardiac muscle. Keywords: Sarcocystis, Haliaeetus albicilla, Oocysts, ITS1, Cox1, Phylogeny |
format |
Article in Journal/Newspaper |
author |
Bjørn Gjerde Turid Vikøren Inger Sofie Hamnes |
author_facet |
Bjørn Gjerde Turid Vikøren Inger Sofie Hamnes |
author_sort |
Bjørn Gjerde |
title |
Molecular identification of Sarcocystis halieti n. sp., Sarcocystis lari and Sarcocystis truncata in the intestine of a white-tailed sea eagle (Haliaeetus albicilla) in Norway |
title_short |
Molecular identification of Sarcocystis halieti n. sp., Sarcocystis lari and Sarcocystis truncata in the intestine of a white-tailed sea eagle (Haliaeetus albicilla) in Norway |
title_full |
Molecular identification of Sarcocystis halieti n. sp., Sarcocystis lari and Sarcocystis truncata in the intestine of a white-tailed sea eagle (Haliaeetus albicilla) in Norway |
title_fullStr |
Molecular identification of Sarcocystis halieti n. sp., Sarcocystis lari and Sarcocystis truncata in the intestine of a white-tailed sea eagle (Haliaeetus albicilla) in Norway |
title_full_unstemmed |
Molecular identification of Sarcocystis halieti n. sp., Sarcocystis lari and Sarcocystis truncata in the intestine of a white-tailed sea eagle (Haliaeetus albicilla) in Norway |
title_sort |
molecular identification of sarcocystis halieti n. sp., sarcocystis lari and sarcocystis truncata in the intestine of a white-tailed sea eagle (haliaeetus albicilla) in norway |
publisher |
Elsevier |
publishDate |
2018 |
url |
https://doi.org/10.1016/j.ijppaw.2017.12.001 https://doaj.org/article/8c9e1ae06eb74cbc9606f34a19ef538f |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Haliaeetus albicilla Lynx |
genre_facet |
Haliaeetus albicilla Lynx |
op_source |
International Journal for Parasitology: Parasites and Wildlife, Vol 7, Iss 1, Pp 1-11 (2018) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2213224417301335 https://doaj.org/toc/2213-2244 2213-2244 doi:10.1016/j.ijppaw.2017.12.001 https://doaj.org/article/8c9e1ae06eb74cbc9606f34a19ef538f |
op_doi |
https://doi.org/10.1016/j.ijppaw.2017.12.001 |
container_title |
International Journal for Parasitology: Parasites and Wildlife |
container_volume |
7 |
container_issue |
1 |
container_start_page |
1 |
op_container_end_page |
11 |
_version_ |
1766022425887113216 |