Rice Husk as an Inexpensive Renewable Immobilization Carrier for Biocatalysts Employed in the Food, Cosmetic and Polymer Sectors
The high cost and environmental impact of fossil-based organic carriers represent a critical bottleneck to their use in large-scale industrial processes. The present study demonstrates the applicability of rice husk as inexpensive renewable carrier for the immobilization of enzymes applicable sector...
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ftdoajarticles:oai:doaj.org/article:8c9a8a3faa6f49529405482ed43e3810 2023-05-15T13:59:45+02:00 Rice Husk as an Inexpensive Renewable Immobilization Carrier for Biocatalysts Employed in the Food, Cosmetic and Polymer Sectors Marco Cespugli Simone Lotteria Luciano Navarini Valentina Lonzarich Lorenzo Del Terra Francesca Vita Marina Zweyer Giovanna Baldini Valerio Ferrario Cynthia Ebert Lucia Gardossi 2018-10-01T00:00:00Z https://doi.org/10.3390/catal8100471 https://doaj.org/article/8c9a8a3faa6f49529405482ed43e3810 EN eng MDPI AG http://www.mdpi.com/2073-4344/8/10/471 https://doaj.org/toc/2073-4344 2073-4344 doi:10.3390/catal8100471 https://doaj.org/article/8c9a8a3faa6f49529405482ed43e3810 Catalysts, Vol 8, Iss 10, p 471 (2018) covalent immobilization of enzymes rice husk polycondensation CaLB itaconic acid aspartase acrylamide renewable carriers biomass Chemical technology TP1-1185 Chemistry QD1-999 article 2018 ftdoajarticles https://doi.org/10.3390/catal8100471 2022-12-31T14:04:35Z The high cost and environmental impact of fossil-based organic carriers represent a critical bottleneck to their use in large-scale industrial processes. The present study demonstrates the applicability of rice husk as inexpensive renewable carrier for the immobilization of enzymes applicable sectors where the covalent anchorage of the protein is a pre-requisite for preventing protein contamination while assuring the recyclability. Rice husk was oxidized and then functionalized with a di-amino spacer. The morphological characterization shed light on the properties that affect the functionalization processes. Lipase B from Candida antarctica (CaLB) and two commercial asparaginases were immobilized covalently achieving higher immobilization yield than previously reported. All enzymes were immobilized also on commercial epoxy methacrylic resins and the CaLB immobilized on rice husk demonstrated a higher efficiency in the solvent-free polycondensation of dimethylitaconate. CaLB on rice husk appears particularly suitable for applications in highly viscous processes because of the unusual combination of its low density and remarkable mechanical robustness. In the case of the two asparaginases, the biocatalyst immobilized on rice husk performed in aqueous solution at least as efficiently as the enzyme immobilized on methacrylic resins, although the rice husk loaded a lower amount of protein. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Anchorage Catalysts 8 10 471 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
covalent immobilization of enzymes rice husk polycondensation CaLB itaconic acid aspartase acrylamide renewable carriers biomass Chemical technology TP1-1185 Chemistry QD1-999 |
spellingShingle |
covalent immobilization of enzymes rice husk polycondensation CaLB itaconic acid aspartase acrylamide renewable carriers biomass Chemical technology TP1-1185 Chemistry QD1-999 Marco Cespugli Simone Lotteria Luciano Navarini Valentina Lonzarich Lorenzo Del Terra Francesca Vita Marina Zweyer Giovanna Baldini Valerio Ferrario Cynthia Ebert Lucia Gardossi Rice Husk as an Inexpensive Renewable Immobilization Carrier for Biocatalysts Employed in the Food, Cosmetic and Polymer Sectors |
topic_facet |
covalent immobilization of enzymes rice husk polycondensation CaLB itaconic acid aspartase acrylamide renewable carriers biomass Chemical technology TP1-1185 Chemistry QD1-999 |
description |
The high cost and environmental impact of fossil-based organic carriers represent a critical bottleneck to their use in large-scale industrial processes. The present study demonstrates the applicability of rice husk as inexpensive renewable carrier for the immobilization of enzymes applicable sectors where the covalent anchorage of the protein is a pre-requisite for preventing protein contamination while assuring the recyclability. Rice husk was oxidized and then functionalized with a di-amino spacer. The morphological characterization shed light on the properties that affect the functionalization processes. Lipase B from Candida antarctica (CaLB) and two commercial asparaginases were immobilized covalently achieving higher immobilization yield than previously reported. All enzymes were immobilized also on commercial epoxy methacrylic resins and the CaLB immobilized on rice husk demonstrated a higher efficiency in the solvent-free polycondensation of dimethylitaconate. CaLB on rice husk appears particularly suitable for applications in highly viscous processes because of the unusual combination of its low density and remarkable mechanical robustness. In the case of the two asparaginases, the biocatalyst immobilized on rice husk performed in aqueous solution at least as efficiently as the enzyme immobilized on methacrylic resins, although the rice husk loaded a lower amount of protein. |
format |
Article in Journal/Newspaper |
author |
Marco Cespugli Simone Lotteria Luciano Navarini Valentina Lonzarich Lorenzo Del Terra Francesca Vita Marina Zweyer Giovanna Baldini Valerio Ferrario Cynthia Ebert Lucia Gardossi |
author_facet |
Marco Cespugli Simone Lotteria Luciano Navarini Valentina Lonzarich Lorenzo Del Terra Francesca Vita Marina Zweyer Giovanna Baldini Valerio Ferrario Cynthia Ebert Lucia Gardossi |
author_sort |
Marco Cespugli |
title |
Rice Husk as an Inexpensive Renewable Immobilization Carrier for Biocatalysts Employed in the Food, Cosmetic and Polymer Sectors |
title_short |
Rice Husk as an Inexpensive Renewable Immobilization Carrier for Biocatalysts Employed in the Food, Cosmetic and Polymer Sectors |
title_full |
Rice Husk as an Inexpensive Renewable Immobilization Carrier for Biocatalysts Employed in the Food, Cosmetic and Polymer Sectors |
title_fullStr |
Rice Husk as an Inexpensive Renewable Immobilization Carrier for Biocatalysts Employed in the Food, Cosmetic and Polymer Sectors |
title_full_unstemmed |
Rice Husk as an Inexpensive Renewable Immobilization Carrier for Biocatalysts Employed in the Food, Cosmetic and Polymer Sectors |
title_sort |
rice husk as an inexpensive renewable immobilization carrier for biocatalysts employed in the food, cosmetic and polymer sectors |
publisher |
MDPI AG |
publishDate |
2018 |
url |
https://doi.org/10.3390/catal8100471 https://doaj.org/article/8c9a8a3faa6f49529405482ed43e3810 |
geographic |
Anchorage |
geographic_facet |
Anchorage |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Catalysts, Vol 8, Iss 10, p 471 (2018) |
op_relation |
http://www.mdpi.com/2073-4344/8/10/471 https://doaj.org/toc/2073-4344 2073-4344 doi:10.3390/catal8100471 https://doaj.org/article/8c9a8a3faa6f49529405482ed43e3810 |
op_doi |
https://doi.org/10.3390/catal8100471 |
container_title |
Catalysts |
container_volume |
8 |
container_issue |
10 |
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471 |
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1766268509530095616 |